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Citations for Electron Microscopy Sciences :
351 - 400 of 498 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of γ-tubulin mixture was pipetted onto EM grids (Electron Microscopy Sciences, Catalog number: CF400-Cu), which were glow discharged for 25 seconds ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were washed twice in MOPS buffer for 5 min and fixed in glutaraldehyde solution (EMS, Hatfield, PA) 2.5% in 100 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) for 5 min and fixed with PFA (1.6%, Paraformaldehyde 16% solution EM grade, Electron Microscopy Sciences, 15710) before freezing ...
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bioRxiv - Neuroscience 2020Quote: ... Ribbons of ~5 sections were oriented and collected on formvar/carbon coated nickel slot grids (Electron Microscopy Sciences) using a perfect loop (Electron Microscopy Sciences).
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bioRxiv - Plant Biology 2022Quote: ... seedlings were washed twice in MOPS buffer for 5 min and fixed in glutaraldehyde solution (EMS, Hatfield, PA) 2.5% in 100 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed by adding a warm double strength fixative solution (5% glutaraldehyde (GA, Electron Microscopy Sciences, #16310) and 4% paraformaldehyde (PFA ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of each fixed sample was deposited onto carbon-coated copper grids (Electron Microscopy Sciences, CF400-Cu). Grids were UV-glow discharged immediately prior to sample addition ...
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bioRxiv - Developmental Biology 2024Quote: Ablation was done using a pair of sharpened dissecting forceps (Electron Microscopy Sciences, style 5, no. 72701-D) on stage 2 floral meristems on dissected inflorescences ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µm FFPE pancreas sections were mounted onto 22 mm x 22 mm glass coverslips (Electron Microscopy Sciences) coated in 0.1% poly-L-lysine (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... they were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, cat. no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anesthetized larvae were moved to a rectangular plate (4.5 cm x 3 cm x 1 cm) coated with Sylgard (Electron Microscopy Sciences, Hatfield, PA). After removing excess water ...
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bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... and washed again twice for 5 minutes each in PBS before coverslipping with Fluoro-Gel (#17985, Electron Microscopy Sciences). Primary antibodies used include ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples (5 μL) were loaded onto a glow discharged Formvar/carbon-coated copper grid (400 mesh, Electron Microscopy Sciences) and stained with 2% uranyl formate ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently mounted on glass slides in Citifluor Mountant Solution (Electron Microscopy Sciences, cat no. AF100-5 & 17977-150). Slides were imaged on a W1-Tokogawa spinning disk confocal microscope using a Nikon 100x ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Bioengineering 2024Quote: ... before being fixed in pre-warmed (5 min in 37° C water bath) 4% formaldehyde (Electron Microscopy Sciences, 15710) in PBS solution for 5 min and permeabilized in pre-warmed (to 37° C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with ddH2O three times 5 min and incubated in 1% thiocarbohydrazide (EMS, cat# 2231-57-4) diluted in ddH2O for 20 min ...
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bioRxiv - Biophysics 2023Quote: ... Grids were picked up from the glass bottom MatTek dish using 5/15 Style Dumont Tweezers (Electron Microscopy Sciences), transferred to their respective tweezers and placed into the EM GP chamber set at 25°C and 99% humidity or the CP3 chamber ...
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bioRxiv - Bioengineering 2024Quote: ... Micelle solution (5 µM) was added to a carbon support TEM grid (200 mesh, Electron Microscopy Sciences, Hatfield, PA). After 5 minutes of incubation ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Brain sections (5 μm thick) were prepared and stained with Luxol Fast Blue (Electron Microscopy Sciences, Hatfield, PA, USA). Slides were kept in LFB solution and incubated at 58°C for 16 hours.
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... After rinsing in 0.1 M PBS at room temperature (10 min x 3) and post-fixed in a sequence of aqueous 1% OSO4 (#19150, Electron Microscopy Science, Hatfield, PA) in 0.1 M PBS ...
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bioRxiv - Cell Biology 2024Quote: ... coli was washed 3 times in PBS and then immediately used live in phagocytic assays or fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA) in PBS and stored for a few weeks.
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bioRxiv - Genomics 2020Quote: Ten million cells from two MSC preparations at early (P2 and 3) and late (P7 and 9) passages were cross-linked with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min. ...
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bioRxiv - Cell Biology 2021Quote: Immediately after cold exposure mice were anaesthetized with isoflurane (3%) and perfused with heparinized saline followed by 4% paraformaldefyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA). Fat pads were carefully dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...