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Citations for Electron Microscopy Sciences :
301 - 350 of 377 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... We then bound the chips to a 24×50×1.5mm glass slide (Fisherbrand 12-544-EP) after activating both surfaces for 10s at 100W in a plasma oven (EMS Quorum 1050X). We found that carefully optimizing plasma parameters and controlling humidity were critical for good binding ...
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bioRxiv - Neuroscience 2023Quote: ... the preparations were optically cleared in 25% glycerol for 1 hr at 25 °C and then mounted on Superfrost slides (Cat# 12-550-143, Fisherbrand) with bridged Glass Cover Slips #0 (Cat# 72198-10, Electron Microscopy Sciences) in Fluoromount-G mounting media (Cat# 0100-01 ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Neuroscience 2023Quote: ... the monkeys were deeply anesthetized (ketamine, 10 mg/kg and sodium pentobarbital, 100 mg/kg) and transcardially perfused with 4% formaldehyde (EMS, Hatfield, PA) in Phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... from native aggregation assays sonicated in a water bath for 10-15 s were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 s using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... sections were dehydrated in a series of graded (25, 50, 75, 100, 100, 100 v/v%, 10 min each) acetonitrile (Electron Microscopy Sciences) aqueous solutions ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Cancer Biology 2022Quote: ... and embedded in resin mixture of EMbed 812 Kit (Electron Microscopy Sciences cat#: 14120), cured in a 60°C oven ...
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bioRxiv - Zoology 2022Quote: ... Samples were embedded with Spurr's Low Viscosity Embedding Media Kit (Electron Microscopy Sciences #14300), following the “Firm” formulation provided by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Microbiology 2019Quote: ... Negative staining of the fractions from OptiPrep density gradients was performed by adsorbing 10 μL of each fraction to glow-discharged 400 mesh ultra-thin carbon coated grids (EMS CF400-CU-UL) for two minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 μm Pore Polyester Membrane Insert fixed to a custom 3D printed insert (Supplemental Material) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10).
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Each explant was mounted onto an O-ring (McMaster Carr 1283N264) fixed into a 60mm dish (Cellstar 628160) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10). Each region was imaged with a 25X objective and cells were counted on a maximal intensity projection ...
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bioRxiv - Microbiology 2024Quote: ... the wells were fixed by adding on top of the supernatant 10% formaldehyde (Formaldehyde Aqueous Solution EM grade, 32%, Liquid Electron Microscopy Sciences, # 15700) at a volume-to-volume ratio for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
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bioRxiv - Neuroscience 2019Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences, cat.no. 14340) by sequential immersions in 50 ...
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bioRxiv - Neuroscience 2020Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences Cat#14340) by sequential immersions in 50 ...
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bioRxiv - Neuroscience 2020Quote: ... the whole brain was impregnated with Lowicryl HM20 Resin Kits (Electron Microscopy Sciences, cat.no. 14340) by sequential immersions in 50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-embedding enhancement was realized with silver enhancement kit (AURION R-Gent SE-EM, EMS) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Pre-embedding enhancement was realized with silver enhancement kit (AURION R-Gent SE-EM, EMS) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Samples (10 µl) were adsorbed to glow-discharged 400 mesh carbon coated ultra-thin grids (Electron Microscopy Sciences 215-412-8400 CF400-CU µL) for 5 min ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Genomics 2022Quote: ... OCT tissue blocks were sectioned into 10 µm thickness sections and placed in the center of poly-L-lysine slides (Electron Microscopy Sciences, 63478-AS), and shipped with dry ice ...
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bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Physiology 2019Quote: ... WT mice were deeply anesthetized with isoflurane and transcardially perfused with 10 ml of PBS followed by 100 ml of PB containing 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA), 2.5% glutaraldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
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bioRxiv - Microbiology 2022Quote: ... Five µL of OMVs that had been diluted 1:10 in saline were added to a glow discharged 200 mesh formvar/carbon grid (Electron Microscopy Sciences, FCF200-Cu) and incubated for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... After rinsing in 0.1 M PBS at room temperature (10 min x 3) and post-fixed in a sequence of aqueous 1% OSO4 (#19150, Electron Microscopy Science, Hatfield, PA) in 0.1 M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Muscles were further incubated in Spurr:acetone prepared using the Spurr Resin kit (Electron Microscopy Science, 14300), at ratios of 1:2 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were silver-enhanced with AURION R-GENT SE-EM kit (Electron microscopy sciences, Cat # 500.033) for 25 mins ...
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bioRxiv - Cell Biology 2022Quote: ... cells were embedded with Spurr’s Low Viscosity Embedding Kit (cat. no. EM0300-1KT, Electron Microscopy Sciences), sectioned (70-90 nm thick) ...