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Citations for Electron Microscopy Sciences :
301 - 350 of 420 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and lysed by vortexing for 10 minutes with cell disruptor beads (0.1-mm diameter; Electron Microscopy Sciences, Hatfield, PA). Lysate was transferred to an 8ml glass vial with an additional 1ml cold methanol rinse of the lysis tube ...
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bioRxiv - Microbiology 2022Quote: ... The TLPs were adsorbed for 10 min on carbon-coated Parlodion films mounted on 300-mesh copper grids (EMS). Samples were washed once with water ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were excised and fixed for 10 min in 4% (w/v) paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed in D-PBS and treated with Sodium Citrate based-R-buffer A (EMS, #62706-10) in 2100-Retriever (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... Sympathetic ganglia were whole-mounted to a slide with Fluoro-Gel with Tris Buffer (Electron Microscopy Sciences, 17985-10).
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bioRxiv - Genetics 2023Quote: ... coverslips were washed with PBS and fixed at RT for 10 minutes with 4% PFA (Electron Microscopy Sciences, 15713S). After fixation ...
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bioRxiv - Neuroscience 2023Quote: bdEV preparations (10 μL) were adsorbed to glow-discharged 400 mesh ultrathin carbon-coated grids (EMS CF400-CU-UL) for 2 minutes following our published protocol (Huang et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue was then rinsed in acetate buffer and dehydrated through a graded series of 10-minute ethanol rinses prior to infiltration in EmBED812 epoxy resin using the manufacturer’s hard resin formulation (EMS). Finally ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed and coverslipped with Fluoro-Gel Mounting Medium with Tris Buffer (catalog #17985-10, Electron Microscopy Sciences). Primary antibodies included in this study were ...
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bioRxiv - Immunology 2021Quote: ... and mounted on the slide using 10 μl of FLUORO-GEL II mounting medium with DAPI (EMS; Cat.# 17985-51) and visualized using an Axio Observer Z1 fluorescence microscope (Zeiss) ...
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bioRxiv - Physiology 2020Quote: ... a few 10 µm thick sagittal cryosections were collected on adhesive slides (Plus gold slide, Electron Microscopy Sciences, Hatfield, PA) and stored for up to 30 min in ice-cold PBS prior to AFM analysis.
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bioRxiv - Neuroscience 2020Quote: ... 10 μl of fibril sample (50 μM) was spotted on carbon-coated Formvar EM grids (Electron Microscopy Sciences, PA, USA), incubated for 5 mins and stained with uranyl formate (1% v/v) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell were then fixed for 10 minutes at room temperature in PBSM + 4% paraformaldehyde (Electron Microscopy Sciences 50-980-492). Following fixation ...
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bioRxiv - Biochemistry 2020Quote: 5 μL of purified sample was applied to glow-discharged (10s) Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge-frozen in liquid nitrogen-cooled liquid ethane ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of the sample was applied to glow-discharged (10 s) Quantfoil R1.2/1.3 holey carbon grids (Au 400, Electron Microscopy Sciences) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μL of samples were mounted on Formvar/carbon film-coated mesh nickel grids (Electron Microscopy Sciences, Hatfield, PA, USA) and left standing for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... Post infection coverslips were rinsed in PBS then fixed for 10 minutes in 4% Paraformaldehyde in PBS (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: EV preparations (10 µL) were adsorbed to glow-discharged 400 mesh ultra-thin carbon coated grids (EMS CF400-CU-UL) for two minutes ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Molecular Biology 2019Quote: ... in PBS (+Ca2+/Mg2+) for 10 min and directly fixed afterwards with 2,4% paraformaldehyde (PFA; Electron Microscopy Sciences; cat.# 15710) in PBS (+Ca2+/Mg2+ ...
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bioRxiv - Microbiology 2022Quote: EV preparations (10 μL) were adsorbed to glow-discharged 400 mesh ultra-thin carbon-coated grids (EMS CF400-CU-UL) for 2 min followed by 3 rinses in TBS and staining in 1% uranyl acetate with 0.05 Tylose ...
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bioRxiv - Cell Biology 2022Quote: ... After washing with 1XPBS for 10 min the stained guts were mounted in Fluoromount-G Mounting Medium (Electron Microscopy Sciences). Stained posterior midguts were imaged in the R5 region using LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of the GII.4 RdRp (diluted 10 times) was adsorbed onto a glow discharged grid carbon-coated grid (CF200-CU; EMS) for 60 s ...
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bioRxiv - Neuroscience 2023Quote: ... Antigens were retrieved through immersion in 10 mM sodium citrate buffer (0.05% Tween 20, pH 6.0) in a pressure cooker (Retriever 2100, Electron Microscopy Sciences) at 120 °C for 20 min and cooled to room temperature for 100 min ...
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bioRxiv - Microbiology 2023Quote: 4 tubes of 10 mL each were inoculated overnight for approximately 18 hours (or the 40 mL of culture from EMS (-) strains were used) ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Biophysics 2023Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed and coverslipped with Fluoro-Gel Mounting Medium with Tris Buffer (catalog #17985-10, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Cell Biology 2019Quote: The following reagents/primary antibodies were used for immunofluorescence analyses: paraformaldehyde solution (10%) was purchased from Electron Microscopy Sciences (Hatfield, PA); mouse anti-TfR2 ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
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bioRxiv - Biophysics 2020Quote: For TEM imaging 10 μL of recombinant tau fibril samples were applied to copper grid (Electron Microscopy Science, FCF-300-Cu) cleaned with plasma for 20 s ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
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bioRxiv - Cell Biology 2019Quote: ... sulfated proteoglycans were fixed in 10% formalin and stained for 30 minutes with 1% Alcian Blue 8GS solution (Electron Microscopy Sciences) on day 12 after plating ...
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bioRxiv - Neuroscience 2021Quote: ... to be non-responsive to toe pinch and transcardially perfused with 10 ml of 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences (EMS)) to flush the vasculature followed by buffered fixative made of 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was washed with MQ water and stained for 20 min with 10 μl 0.1% uranyl formate solution (Electron Microscopy Sciences). The uranyl formate solution was prepared freshly and filtered with a 0.22 μm sterile syringe filter (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (10 µL) of fixed cells were then loaded onto PTFE printed glass slides (cat#63429-04 from Electron Microscopy Sciences) with 4-mm diameter wells and allowed to air-dry ...
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bioRxiv - Biophysics 2023Quote: ... one quarter of the bell from each medusae was chemically fixed in 10 mL of 2.5% [v/v] glutaraldehyde (Electron Microscopy Sciences), 4% [v/v] paraformaldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Cell Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.