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Citations for Electron Microscopy Sciences :
301 - 350 of 653 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The as2-7D mutant in the Ler-0 background was identified from ethyl methanesulfonate (EMS) based forward genetic screen ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5% (w/v) benzoin ethyl ether (Electron Microscopy Sciences, Cat. No. 11280, Hatfield, PA, USA) was added to the methacrylate monomer solution and mixed for at least 10 min ...
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bioRxiv - Genetics 2023Quote: ... and leucine (20 mg/L) were performed to examine the effect of ethyl methanesulfonate (EMS), sodium dodecyl sulfate (SDS) ...
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bioRxiv - Microbiology 2024Quote: ... thaliana NG2 seeds were incubated with 0.3% ethyl methane sulphonate (EMS, Sigma-Aldrich; M0880-1G) overnight as previously described (33) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
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bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
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bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were rinsed 3 times with PBS++ and fixed with 2% paraformaldehyde (PFA; 15710, Electron Microscopy Sciences) in PBS++ for 15 min at R.T ...
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bioRxiv - Immunology 2024Quote: ... the 3 μl mixture was applied to a UltrAuFoil R1.2/1.3 300 mesh grid (Electron Microscopy Sciences) that had been glow-discharged with a PELCO easiGlow glow discharge cleaning system for 1 min ...
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bioRxiv - Cell Biology 2020Quote: We treated L4 and young adult xdIs109 (Punc-119::PLIN1::GFP) worms with ethyl methanesulfonate (EMS). About 2,500 F1 were screened ...
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bioRxiv - Microbiology 2021Quote: The ΔprkAcond strain was mutagenized by a 10-minute exposure to 17.8 mM ethyl methanesulfonate (EMS) following a protocol previously described [59 ...
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bioRxiv - Developmental Biology 2021Quote: ... Marenda (Drexel University, Philadelphia, PA)—has a null allele of kis created by ethyl methanesulfonate (EMS) mutagenesis (38) ...
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bioRxiv - Systems Biology 2020Quote: Pacdh-1::GFP;nhr-10(tm4695) animals were treated with 50 mM ethyl methanesulfonate (EMS, Sigma) for four hours and then washed five times with M9 buffer ...
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bioRxiv - Genetics 2023Quote: ... hsp-6p::GFP animals at the L4 stage were incubated with Ethyl-methyl sulfonate (EMS, Sigma). Five plates of 250 F1 progeny each were selected to screen 2500 genomes ...
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bioRxiv - Immunology 2023Quote: ... The worm pellet was resuspended in 4 ml M9 and 25 µl of ethyl methanesulfonate (EMS) was added to the tube (45) ...
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bioRxiv - Microbiology 2024Quote: ... Late-stage parental parasites were incubated with 0-1.5 mM ethyl methanesulfonate (EMS, 8 concentrations total) for 2 hrs and then washed 3x with growth media ...
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bioRxiv - Cancer Biology 2022Quote: ... washed and fixed O/N in 4% paraformaldehyde/phosphate buffered saline (PBS) solution (EMS). Left lung lobes were then treated for paraffin inclusion ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Biophysics 2022Quote: 3 µL of sample was pipetted onto Quantifoil R1.2/R1.3 holey carbon grids (Cu 400, Electron Microscopy Sciences) which had been glow discharged for 1 min using a PELCO easiGlow glow discharge cleaning system (Ted Pella) ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times with 1X PBS and mounted on glass slides using Hydromount (Electron Microscopy Sciences).
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bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
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bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Microbiology 2024Quote: EM grids coated with a 3–4 nm thick carbon film (CF300-CU-UL, Electron Microscopy Sciences, USA) were treated in an UV/ozone chamber (ProCleaner ...
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bioRxiv - Genetics 2024Quote: Testes were dissected and placed into 3 ml of 4% PFA (16% stock, Electron Microscopy Sciences, REF-15710) in a 15 ml falcon tube ...
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bioRxiv - Biophysics 2024Quote: ... The grids were negatively stained for one minute using 3.5 µL of 3% uranyl acetate (Electron Microscopy Sciences). Excess liquid was blotted off using filter paper and the grids were dried overnight before imaging ...
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bioRxiv - Bioengineering 2021Quote: Random mutagenesis was performed on a population of wild-type worms by exposure to ethyl methanesulfonate (EMS) using standard protocols(36) ...
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bioRxiv - Neuroscience 2022Quote: ... flp-13pr::ACR-12::GFP strain IZ1905 animals were treated with 25 µM ethyl methanesulfonate (EMS, Sigma). After washing ...
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bioRxiv - Genetics 2023Quote: ... For the genetic suppressor screens approximately 32,000 Bur-0 seeds were mutagenized with ethyl methane sulfonate (EMS) as described previously 5 ...
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bioRxiv - Neuroscience 2024Quote: ... Animals carrying a PVD::GFP transgene and rab-10 mutation were mutagenized with 50mM ethyl methanesulfonate (EMS). F2 animals were visually screened using a fluorescent compound microscope to identify mutants with restored dendritic branching ...
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bioRxiv - Developmental Biology 2020Quote: Organoid cultures were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: Washed platelets in a concentration of 3 x 105 platelets/μl were fixed with 2.5% glutaraldehyde (Electron Microscopy Science) in cacodylate buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of LcTwinkle-DNA sample was deposited onto a freshly glow-discharged CF400-CU carbon grid (EMS Inc.). After absorbing the extra protein solution with filter paper ...
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bioRxiv - Cell Biology 2022Quote: ... as model cargos and placed in the incubator for 15 min or 3 h incubation followed by washing with PBS and fixation with 2.5% glutaraldehyde (EMS) in 0.1 M phosphate or cacodylate buffer ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... 3 uL of sample was blotted on glow-discharged UltraAuFoil EM-grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences), with 3 s of wait time and 7 sec of blot time ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of this suspension was deposited on glow-discharged 300 mesh formvar/carbon copper grids (Electron Microscopy Sciences), washed once with water and stained with 0.5% w/v phosphotungstic acid ...
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bioRxiv - Developmental Biology 2024Quote: Tissues were fixed between 15 minutes to 3 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Post-fixation samples were dehydrated with ethanol ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), allowed to incubate for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... a 3 μL sample was placed on a glow-discharged carbon film 300-mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were grown for 3 hours with vigorous shaking every 30 minutes (to keep the EMS in solution). The cultures were passed out of the chamber and centrifuged at 3,000 x g for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... culture aliquots were taken and mixed 1:2 with Paraformaldehyde 3% Glutaraldehyde 0.35% In 0.1M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Cells were fixed for 30-60 min at RT and diluted in low fluorescence media ...