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Citations for Electron Microscopy Sciences :
3401 - 3450 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... A 10 μl droplet of the EV-PFA mixture was then allowed to adsorb to a carbon-coated copper grid (Electron Microscopy Sciences, CF200-Cu-25) for 20 min ...
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bioRxiv - Bioengineering 2022Quote: ... 350 µl of 300 mM sodium cacodylate pH 7.2 (Electron Microscopy Sciences) was added ...
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bioRxiv - Bioengineering 2022Quote: ... The denatured RRE RNA was mixed with 612 µl of 300 mM sodium cacodylate pH 7.2 (Electron Microscopy Sciences) and incubated at 37°C for 20 min to refold its structure ...
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bioRxiv - Bioengineering 2022Quote: ... Paraformaldehyde Aqueous Solution was purchased from Electron Microscopy Sciences (Hatfield, PA, USA). Odyssey® PBS Blocking Buffer was purchased from LI-COR Biosciences (Linkoln ...
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bioRxiv - Biophysics 2022Quote: ... C-flat holey carbon 1.2/1.3 grids (Electron Microscopy Sciences) or UltrAuFoil holey gold film grids (Ted Pella ...
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bioRxiv - Cell Biology 2022Quote: ... a ∼1.0 mm3 piece was trimmed of any excess resin and mounted to an 8 mm aluminum stub using silver epoxy Epo-Tek (EMS, Hatfield, PA). The mounted sample was then carefully trimmed into a smaller ∼0.5 mm3 tower using a Diatome diamond trimming tool (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... The mounted sample was then carefully trimmed into a smaller ∼0.5 mm3 tower using a Diatome diamond trimming tool (EMS, Hatfield, PA) and vacuum sputter-coated with gold palladium to help dissipate charge.
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bioRxiv - Cell Biology 2022Quote: Four μm kidney cortices optimal cutting temperature (OCT) compound embedded sections were incubated with 100 μl freshly prepared Oil Red O solution (Electron Microscopy Science, Hatfield, PA) for 15 minutes and counterstained with Hematoxylin Harris solution (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... 200 nm thickness sections were collected on slotted copper grids (Electron Microscopy Sciences, Hatfield, PA) coated with formvar membrane ...
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bioRxiv - Cell Biology 2022Quote: ... processed in a standard manner and embedded in EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). 70 nm ultrathin sections were cut (UC6 microtome ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Cell Biology 2022Quote: ... Ovarioles were separated from the muscle sheath using Dumont style 5 stainless steel forceps (Electron Microscopy Sciences) before fixation ...
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bioRxiv - Cell Biology 2022Quote: ... and mounted with Fluoromount-G™ (Electron Microscopy Sciences #17984-25). The immunostaining of 3D-endothelial differentiated cells was performed following the protocol of (Fernandez-Alonso et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Sample (3 μl) was loaded onto a glow-discharged QUANTIFOIL grids (Q250-CR1.3, EMS) and flash-frozen using Vitrobot (Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05 mg/mL) were applied on the carbon side of glow-discharged carbon-coated holey copper grids (EMS CF300-CU). After 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... The pixel sizes at nominal magnifications from 14,000× to 120,000× were calibrated using images acquired of a crossed line grating replica (Electron Microscopy Sciences, Hatfield, PA USA), processed with DE_MagCal.ijm macro (available at https://github.com/directelectron/imagej-macros ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... the coverslips with cells were incubated in 2% osmium tetroxide (Electron Microscopy Sciences, Hatfield PA, US) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... a carbon-coated electron microscopy grid (CF300-Cu, Electron Microscopy Sciences), carbon side down ...
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bioRxiv - Bioengineering 2022Quote: ... These samples were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA) in sterile PBS for 30 minutes on an orbital rocker at 37 ºC ...
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bioRxiv - Bioengineering 2022Quote: ... Formvar carbon-coated copper grid (FCF400Cu, Electron Microscopy Sciences) which was subsequently blotted against filter paper and negative stained ...
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bioRxiv - Cancer Biology 2022Quote: ... and then slides were mounted with Fluoro-Gel (EMS, 17985-50).
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bioRxiv - Bioengineering 2022Quote: 4% paraformaldehyde (Electron Microscopy Sciences) was used for cell fixation before permeabilization with 0.1% Triton X-100 (Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 6-8 μl of sample were applied on a freshly glow-discharged grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11, Electron Microscopy Science). The carbon layer was floated on the water surface and then transferred to the cryo-EM grids by raising them to the water surface under the carbon layer ...
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bioRxiv - Biophysics 2022Quote: ... they were rinsed in PBS and fixed with 4% PFA (Electron Microscopy Sciences) for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... media was removed from the plates and immediately fixed using 2.5% Glutaraldehyde 2.5% Paraformaldehyde in 0.1 M sodium cacodylate buffer at pH 7.4 (Electron Microscopy Sciences, 15949) for at least 1 hour at room temperature before embedding.
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bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
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bioRxiv - Biophysics 2022Quote: ... the PDMS was detached from the master mold and access holes for the tubing connections were perforated using a surgical puncher with a diameter of 1.5 mm (Rapid-Core sampling tool, Electron Microscopy Sciences). In addition ...
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bioRxiv - Biophysics 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in 1x PBS for 10 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of sample was blotted on 400 mesh carbon support film (cf400-cu, EMS) for 30 seconds ...
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bioRxiv - Bioengineering 2022Quote: Cells were fixed in 4% paraformaldehyde (PFA, 16% stock, 15710, Electron Microscopy Sciences) diluted in 1x DPBS for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... for 2 days at room temperature (peritoneal cavity) or in 4% paraformaldehyde (Electron Microscopy Sciences) overnight at 4 °C (muscle tissue).
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bioRxiv - Bioengineering 2022Quote: ... rehydrated samples were stained with safranin-O (Electron Microscopy Sciences) and counterstained with fast green (VWE ...
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bioRxiv - Biochemistry 2022Quote: ... paraformaldehyde (Electron Microscopy Sciences, 15710); XF Plasma Membrane Permeabilizer (XF-PMP ...
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bioRxiv - Bioengineering 2022Quote: ... After 24 hours, fibroblasts were fixed with 4% (w/v, in PBS) paraformaldehyde (Electron Microscopy Sciences 15710), permeabilized with 0.1% (w/v ...
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bioRxiv - Bioengineering 2022Quote: 1 mm silicone spacers with 9 mm diameter circular openings (Electron Microscopy Sciences) were adhered to amine coated glass slides (Nanocs) ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were fixed using paraformaldehyde (Electron Microscopy Sciences, USA) after 24 hours ...
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bioRxiv - Bioengineering 2022Quote: PANC-1 cells were cultured on 22×22 mm coverslips (Electron Microscopy Sciences, no. 1.5) for 48 h and fixed with ice cold 4% PFA for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Bioengineering 2022Quote: Cells were fixed with 1% (for Piezo1) or 4 % (for phalloidin) paraformaldehyde (PFA, Electron Microscopy Sciences 15713-S) for 10 minutes and washed two times with PBS ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Bioengineering 2022Quote: ... Liposomes were diluted in ultrapure water and applied to charge coated formvar/carbon coated copper grids (400 mesh, Electron Microscopy Sciences) for 15 minutes followed by washing for 2 seconds with ultrapure water ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... The grid was then positioned on a droplet of uranyl-acetate replacement stain (Electron Microscopy Sciences, 22405) and allowed to dry completely for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Three washing steps with double distilled H2O were followed by three staining steps with 0.75% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were imaged using a FEI Tecnai 10 operated at 100 keV ...
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bioRxiv - Biochemistry 2022Quote: ... 3 uL were pipetted on Quantifoil grids Cu R2/2 (EMS # LFH2100CR2) that were then manually backblotted for 2 seconds and plunge frozen in liquid nitrogen ...