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Citations for Electron Microscopy Sciences :
251 - 300 of 409 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... For immunofluorescence analysis cells were seeded (1.5 x 105 HeLa cells) directly on poly-L-lysine coated coverslips (Electron Microscopy Sciences), which were placed in a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: A drop (1-2 μl) of undiluted EVs (PEG/SEC-enriched EVs from 100 uninjured egfp(RNAi) animals) was placed on a formvar (EMS 15810)-coated hexagonal grid (EMS G300-Cu ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
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bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
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bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were rinsed 3 times with PBS++ and fixed with 2% paraformaldehyde (PFA; 15710, Electron Microscopy Sciences) in PBS++ for 15 min at R.T ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 2 % PFA and allowed to attach on poly-L-lysine-coated 12 mm glass coverslips (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 30 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... sectioned (thickness of 7-10 µm) and put at the center of poly-L-lysine covered glass slides (63478-AS, Electron Microscopy Sciences).
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bioRxiv - Biochemistry 2020Quote: ... An aliquot (approximately 4 μL) of diluted sample was placed on an ultra-thin carbon-coated 200-mesh copper grid (CF200-Cu-UL, Electron Microscopy Sciences, Hatfield ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide fibrils were obtained as described a ove and 5 μl of the sample was spotted on non-glow discharged carbon film 300 mesh copper grids (CF300-CU from Electron Microscopy Sciences) and incubated for 1 min ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by applying 3 – 5 μL of in vitro assembled PLP (with or without PC portal rings) onto 300-mesh carbon coated copper grids (Electron Microscopy Sciences). Samples were absorbed for 1 minute and washed with 2-3 drops of water ...
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bioRxiv - Biophysics 2020Quote: 4 μl of 22 μM tau aggregation samples were adsorbed onto glow-discharged 200 mesh formvar carbon coated copper grids (Electron Microscopy Sciences) and stained with aqueous uranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: A drop of nanoparticle sample obtained from the self-assembly of PEG-block-poly (acetylated L-lysine) co-polypeptide was placed on a 300-mesh Formvar carbon-coated copper TEM grid (Electron Microscopy Sciences) for 1 min and wicked off ...
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bioRxiv - Biophysics 2024Quote: 3 µL diluted MgtA (0.01 mg/mL) was deposited on a carbon-coated 400 mesh copper grid (CF400-CU, EMS, Hatfield, PA, USA) following glow discharging (PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Neuroscience 2024Quote: Gels were then moved to a poly-l-lysine coated cover glass-bottom petri dish (Electron Microscopy Sciences, catalog #70673, Hatfield, PA) with a paintbrush and incubated for expansion in ddH2O for 3∼4 rounds of 20-minute expansion session until expansion plateaued (around 60-100 minutes) ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times with PBS and then incubated with a 4% PFA solution (Electron microscopy sciences) for 30 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Biophysics 2022Quote: 3 µL of sample was pipetted onto Quantifoil R1.2/R1.3 holey carbon grids (Cu 400, Electron Microscopy Sciences) which had been glow discharged for 1 min using a PELCO easiGlow glow discharge cleaning system (Ted Pella) ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were then transferred to a 3:1 mixture of propylene oxide: Araldite resin (EMS, Cat#13940) for overnight incubation under gentle rolling at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times with 1X PBS and mounted on glass slides using Hydromount (Electron Microscopy Sciences).
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bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
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bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Genetics 2024Quote: Testes were dissected and placed into 3 ml of 4% PFA (16% stock, Electron Microscopy Sciences, REF-15710) in a 15 ml falcon tube ...
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bioRxiv - Microbiology 2024Quote: EM grids coated with a 3–4 nm thick carbon film (CF300-CU-UL, Electron Microscopy Sciences, USA) were treated in an UV/ozone chamber (ProCleaner ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of sample solution was applied onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS, Hatfield, PA, USA). The sample solution was left for 30 s before blotted with a piece of filter paper ...
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bioRxiv - Genetics 2020Quote: ... Tissue was transferred to a drop of Schneider’s on a silanized coverslip and gently squashed onto a Poly-L-Lysine coated slide (Electron Microscopy Sciences, Hatfield, PA). Coverslips were immediately removed after freezing in liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... a 3 μL drop of the mixture was placed on a glow discharge continuous carbon grid (Electron Microscopy Science) and stained with 2% uranyl acetate ...
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bioRxiv - Developmental Biology 2020Quote: Organoid cultures were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...