Labshake search
Citations for Electron Microscopy Sciences :
251 - 300 of 1041 citations for 8 DECYLOXYPYRENE 1 3 6 TRISULFONIC ACID TRISODIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... Both channels (n=3 per donor) were fixed using 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences). The PFA solution was pipetted into both channels followed by a 15-minute incubation at room temperature (RT) ...
-
bioRxiv - Neuroscience 2019Quote: bEnd.3 cell monolayers were grown on collagen-coated glass bottom culture dish (Electron Microscopy Sciences). On the day of experiment ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were dried on carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, USA) overnight at room temperature in a desiccator ...
-
bioRxiv - Microbiology 2020Quote: Samples (3 μL) were onto the carbon side of carbon-coated copper grids (Electron Microscopy Sciences) for 90 s ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cells were then harvested and 3×106 cells were fixed in 4% paraformaldehyde (EMS, 15710) at RT for 15 min ...
-
bioRxiv - Biochemistry 2022Quote: ... 3 μl were applied on carbon-coated 200-mesh Cu grids (Electron Microscopy Sciences, Hatfield, PA). After 60 s of incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were passed through 3 separate blocks of optimal cutting temperature (OCT) compound (Electron Microscopy Sciences) to wash off the sucrose solution ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed 3 times with CyTOF buffer and fixed in 1.6% PFA (Electron Microscopy Sciences) at 1×106 cells/ml for 15 minutes at RT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and a 3 µl mixture was spotted on glow-discharged grids (Electron Microscopy Sciences, CF400-Cu), then stained with 2% uranyl acetate ...
-
bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
-
bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
-
bioRxiv - Immunology 2023Quote: ... sections prepared from mouse lungs frozen in OCT were stained with Sirius Red (0.1% Sirius Red in saturated picric acid, Electron Microscopy Sciences, 26357-02) for 60 min followed by a 2-min 0.01 N HCl wash ...
-
bioRxiv - Microbiology 2021Quote: ... GUVs were placed on a 6 mm diameter chamber made from a silicon sheet using a core sampling tool (EMS #69039-60) and imaged prior to protein addition to ensure proper GUV formation and suspension ...
-
bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...
-
bioRxiv - Microbiology 2020Quote: ... and 0.5 M glucose) and placed on 6 mm diameter chambers made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5 mm clean coverglass (EMS # 72200-31 ...
-
bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS, 69039-60). The silicon chamber was mounted on a 1.5-mm clean cover glass (EMS ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
-
bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
-
bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
-
bioRxiv - Cell Biology 2019Quote: ... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...
-
bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
-
bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
-
bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
-
bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
-
bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
-
bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
-
bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
-
bioRxiv - Cell Biology 2023Quote: Wandering third-instar larvae were dissected in ice cold Ca2+-free saline and fixed for 6 minutes in Bouin’s Fixative (Electron Microscopy Sciences, Cat# 15990). Dissections were washed several times with 1X PBS and then permeabilized with 1X PBS containing 0.1% Triton-X (Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times with PBS and then incubated with a 4% PFA solution (Electron microscopy sciences) for 30 min at room temperature (RT) ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...
-
bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
-
bioRxiv - Biophysics 2022Quote: 3 µL of sample was pipetted onto Quantifoil R1.2/R1.3 holey carbon grids (Cu 400, Electron Microscopy Sciences) which had been glow discharged for 1 min using a PELCO easiGlow glow discharge cleaning system (Ted Pella) ...
-
bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
-
bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
-
bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times with 1X PBS and mounted on glass slides using Hydromount (Electron Microscopy Sciences).
-
bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
-
bioRxiv - Cell Biology 2019Quote: ... Cells were allowed to grow for 6 h and then compressed for 18 h before being fixed with 4% paraformaldehyde (# 30525-89-4, Electron Microscopy Sciences, Hatfield, PA). The non-invasive cells on the upper chamber were removed with cotton swabs ...
-
bioRxiv - Neuroscience 2022Quote: ... The mammary gland was excised and fixed overnight from either lactating wild-type (C57BL/6) or oxytocin knockout mice in 4% PFA (Electron Microscopy Sciences, Hatfield, PA). Tissue was incubated at 37 °C in CUBIC Reagent 1 A (10 wt% Triton ...
-
bioRxiv - Genomics 2023Quote: ... cells in the 6 well glass bottom plates were fixed by aspirating the medium and replacing it with 4% paraformaldehyde (Electron Microscopy Sciences, 15710-S) in a 1x RNase free PBS solution made from 10x RNase Free PBS (ThermoFisher ...
-
bioRxiv - Physiology 2024Quote: ... The brain was removed from the skull and a ∼6 mm thick section collected from the optic chiasm to the mamillary bodies using a rat coronal brain matrix (Electron Microscopy Sciences, 69083-C). The hypothalamus was isolated by removing brain matter above the top of the third ventricle and lateral to the optic tract ...