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Citations for Electron Microscopy Sciences :
2901 - 2950 of 2963 citations for 6 Bromo 2 4 iodo phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: For negative staining 3 µl of purified 40S+60S ribosome fractions or alternatively 3 µl of 40S+60S ribosome fractions in the presence of (PR)20 (1:10 molar ratio) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and retained on the grid for 1 min ...
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bioRxiv - Neuroscience 2019Quote: ... and then immersed in 50% ethanol before being dehydrated in ascending concentrations of ethanol (50%, 70%, 90%, 100%) containing 1% uranyl acetate (UA; Electron Microscopy Sciences) with application of microwave irradiation (250 W ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2021Quote: 8 μl of 1 x 1010 pfu/mL of phages was applied to a copper mesh grid coated with formvar and carbon (Electron Microscopy Sciences) for 2 minutes and then gently blotted off with a piece of Whatman filter paper ...
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bioRxiv - Biochemistry 2022Quote: Washed and deglycosylated RBCs were diluted 1:100 and added to carbon coated 400 mesh copper grids (CF400-CU, Electron Microscopy Sciences) that had been glow discharged and pre- coated in poly-d-lysine solution ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were then counterstained with Hoechst 33342 (1:10,000) and mounted using Fluoro-gel in TES Buffer (Electron Microscopy Sciences, 17985-30). The slides were imaged using a Zeiss Observer D1 microscope with a 40x objective.
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bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
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bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
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bioRxiv - Biochemistry 2023Quote: ... In-house graphene grids were prepared from Trivial Transfer Graphene sheets (ACS Material) and QUANTIFOIL R2/1 mesh 300 gold (Electron Microscopy Sciences) by following a protocol introduced by Ahn et. ...
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bioRxiv - Neuroscience 2024Quote: ... made in double distilled H2O (ddH2O) and crosslinked by 1% glutaraldehyde (diluted from 50% glutaraldehyde, Electron Microscopy Sciences, catalog #16320, Hatfield, PA). 40 µm sections were cut on a vibratome (Leica VT1000S ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were washed five times for 10 min each with sterile deionized water and then transferred to fresh solution containing 1% (w/v) thiocarbohydrazide (EMS, Cat# 21900) in deionized water and incubated for 1 hour under rotation at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... were collected on nickel grids using a Leica EM UC7 ultra microtome and stained with uranyl acetate (1%, w/v) (uranyl acetate dihydrate, Electron Microscopy Sciences) and Reynolds lead citrate71 (Lead nitrate (Thermofisher ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
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bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
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bioRxiv - Biophysics 2023Quote: ... samples were taken directly from the microplates (section 2.3) and deposited for 1 min on a previously negative glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, Pennsylvania). The grids were then washed with water and stained with phosphotungstic acid ...
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bioRxiv - Biochemistry 2023Quote: 5 μl of PFF solution (∼1 mg/mL) was spotted on a freshly glow-discharged 300-mesh carbon coated formvar grid (Electron Microscopy Sciences, USA) and incubated for 3 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were suspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% monomeric formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anesthetized larvae were moved to a rectangular plate (4.5 cm x 3 cm x 1 cm) coated with Sylgard (Electron Microscopy Sciences, Hatfield, PA). After removing excess water ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Overnight cultures were diluted 1:10 in fresh LB medium and were added to 48-well microtiter plates containing autoclaved Aclar fluoropolymer film (Electron Microscopy Sciences, Hatfield, PA). Biofilms were grown for 48 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were negatively stained with 1% uranyl acetate in Tris NaCl refolding buffer and fixed onto 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). TEM imaging was done at 100x magnification with a JEOL 1400 electron microscope ...
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bioRxiv - Cell Biology 2021Quote: ... a film of germanium (Structure Probe, Inc., West Chester, PA) was applied to glass coverslips of 25 mm diameter and thickness #1 (Electron Microscopy Sciences, Hatfield, PA) as previously described (Heckman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were adsorbed for 30 s (for high salt conditions) to 1 h in a humid chamber (for low salt conditions) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low salt conditions ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Microbiology 2021Quote: ... Surface colonization was performed by washing and diluting an LB overnight culture 1:100 into fresh SCFM and dispensing 100 µl into coverslip attached open incubation chambers (Electron Microscopy Sciences, 70333-42). The coverslips were incubated in parafilm sealed sterile petri dishes at 37°C and the media was gently exchanged every 4 hours ...