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Citations for Electron Microscopy Sciences :
2651 - 2700 of 3107 citations for 6 Quinoxalinecarboxaldehyde 1 2 3 4 tetrahydro 1 methyl 3 oxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were fixed in a final concentration of 2% paraformaldehyde (Cat# 15714S; Electron Microscopy Sciences) and % GFP positive cells were enumerated by flow cytometry (Intellicyt iQue Screener PLUS ...
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bioRxiv - Cell Biology 2023Quote: ... Post-fixation was performed using 2% (w/v) osmium tetroxide (Electron Microscopy Sciences, Hatfield, UAS) for 30 min in chilled cacodylate buffer ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mg/mL sample was applied to the glow discharged gird (Electron Microscopy Sciences, 25486), followed by removing the extra sample amount using filter paper and quickly plunging into liquid ethane ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Overnight cultures were diluted 1:10 in fresh LB medium and were added to 48-well microtiter plates containing autoclaved Aclar fluoropolymer film (Electron Microscopy Sciences, Hatfield, PA). Biofilms were grown for 48 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were negatively stained with 1% uranyl acetate in Tris NaCl refolding buffer and fixed onto 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). TEM imaging was done at 100x magnification with a JEOL 1400 electron microscope ...
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bioRxiv - Cell Biology 2021Quote: ... a film of germanium (Structure Probe, Inc., West Chester, PA) was applied to glass coverslips of 25 mm diameter and thickness #1 (Electron Microscopy Sciences, Hatfield, PA) as previously described (Heckman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were adsorbed for 30 s (for high salt conditions) to 1 h in a humid chamber (for low salt conditions) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low salt conditions ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Microbiology 2021Quote: ... Surface colonization was performed by washing and diluting an LB overnight culture 1:100 into fresh SCFM and dispensing 100 µl into coverslip attached open incubation chambers (Electron Microscopy Sciences, 70333-42). The coverslips were incubated in parafilm sealed sterile petri dishes at 37°C and the media was gently exchanged every 4 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Five µL of OMVs that had been diluted 1:10 in saline were added to a glow discharged 200 mesh formvar/carbon grid (Electron Microscopy Sciences, FCF200-Cu) and incubated for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were dissected in IMADS (Singleton and Woodruff 1994) or 1X PBS and fixed with 4% PFA (Electron Microscopy Sciences) in 1X PBS plus 0.3% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were first washed with PBS for 5 minutes and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, PA, USA) in PBS containing 0.1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... samples were washed once in wash buffer and fixed with a 4% Paraformaldehyde + 0.1% Glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) solution made in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected larvae were fixed for 20 (VNC) or 45 minutes (NMJs) in 4% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... When animals reached the indicated age they were anaesthetized with isoflurane and intracardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences) in PBS was performed ...
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bioRxiv - Immunology 2019Quote: ... The right-side half brains were harvested and fixed in 4% paraformaldehyde (made in PBS; #15710, Electron Microscopy Sciences, USA) at 4◻ for 6 days ...
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bioRxiv - Biochemistry 2020Quote: ... Cell were then fixed for 10 minutes at room temperature in PBSM + 4% paraformaldehyde (Electron Microscopy Sciences 50-980-492). Following fixation ...
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bioRxiv - Neuroscience 2020Quote: ... perfused intracardially with ice-cold phosphate-buffered saline (PBS) and fixed with 4 % paraformaldehyde (PFA, 19210, Electron Microscopy Sciences, USA) in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: Adult mice were anesthetized with isoflurane and transcardial perfusion was performed with 7.5 ml of 1x PBS following by 7.5 ml of ice cold 4% PFA in 1x PBS (EMS – 15710-S). Brains were then dissected out and post-fixed in 4% PFA in 1x PBS for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Post infection coverslips were rinsed in PBS then fixed for 10 minutes in 4% Paraformaldehyde in PBS (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of parasite culture at 5% parasitemia was washed with PBS and re-suspended in a fresh fixative solution (4% Paraformaldehyde (EMS) and 0.0075% glutaraldehyde (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... and lens were gently removed and the resulting posterior eyecup was fixed in freshly prepared ice-cold 4% (w/v) PFA (171010, EMS) in PBS for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... followed by application of 4 μl of the mix onto glow discharged continuous carbon electron microscopy grid (400 mesh, EMS) and was incubated on grid for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... and then 4 μl of the mixture was added to a Quantifoil R1.2/1.3 Au 300 mesh grid (Electron Microscopy Sciences) that had been glow discharged for 60 seconds at 20 mA using a Pelco easiGlow ...
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bioRxiv - Bioengineering 2022Quote: ... the media is removed from the wells and the cells are fixated via a 4% paraformaldehyde (PFA, Electron Microscopy Sciences) solution incubation for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Fixation buffer consisting of 4% paraformaldehyde (Alfa Aeser, USA, CAT#:43368) and 0.1% glutaraldehyde (Electron Microscopy Sciences, USA, CAT#: 16100) was used to fix cells on the coated surfaces.
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bioRxiv - Neuroscience 2022Quote: ... the recipient mice eyes and cultured retinal organoids were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) in PBS and dehydrated in a sucrose gradient (10% ...
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bioRxiv - Neuroscience 2022Quote: ... animals were transcardially perfused with ice cold 1x PBS followed by ice cold 4% PFA (Electron Microscopy Sciences, 100504-858). Spinal cords were dissected and post-fixed in 4% PFA for 2 hours at 4°C ...
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bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with 1X PBS and fixed in 4% paraformaldehyde (PFA) solution (diluted from 16% PFA, Electron Microscopy Sciences) in PBS for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with ketamine/xylazine before transventricular cardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA; Cat #19210) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2019Quote: ... 10cm long) were gently swiss rolled from the distal end and fixed in 4% paraformaldehyde (Electron Microscopy Science, Hatfield USA). Formalin-fixed tissues were then processed for paraffin embedding ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μL solution in each reaction condition was immediately transferred to a glow-discharged continuous carbon grid (Electron Microscopy Sciences) for 15 seconds before blotting and staining ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with 0.9% NaCl followed by a mixture of 4% PFA and 0.1% glutaraldehyde (Electron Microscopy Sciences) in 0.1M phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Neuroscience 2023Quote: E16.5 and E18.5 embryos were sacrificed by head sectioning and brains were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in Phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Several FA/GA-fix tissue sections were post-fixed with 0.01% osmium tetroxide (4% osmium solution; EMS, catalog no. 19150) in 1× PBS for 1h at RT (FA/GA/OsO4-fix) ...
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bioRxiv - Neuroscience 2022Quote: Anesthetized mice were transcardially perfused with ice cold phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were post-fixed in 4% PFA overnight and cryoprotected in 30% Sucrose (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... temporal bones were immediately dissected to expose the sensory epithelium and fixed in paraformaldehyde (PFA 4%; Electron Microscopy Sciences; 15710) for 1h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: 48 hpf Tg(kdrl:eGFP) and Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-Runx1-eGFP) embryos were fixed in 4% formaldehyde (Electron Microscopy Sciences, Cat#15712) diluted in PBS/0.1% tween20 (PBST ...