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Citations for Electron Microscopy Sciences :
2551 - 2600 of 2708 citations for 6 Fluoro 4 hydroxy 1 7 naphthyridine 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Cell Biology 2022Quote: ... four to five serial sections were picked up per 2 × 1 mm formvar and carbon coated slot grid (EMS). Because the Aclar coverslip ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then rinsed with 0.1 M cacodylate buffer and fixed with 1 % osmium tetroxide (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... MMA/graphene was applied onto 300 mesh gold-coated Quantifoil R 0.6/1 copper grids (Electron Microscopy Sciences, USA) by using the grids to scoop out the bilayer ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for SEM imaging by OTOT method where two consecutive baths of buffered 1% osmium tetroxide (EMS) and 1% tannic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and allowed to completely dry before staining with either a Sato’s Lead solution (1% lead acetate (EMS, Cat# 17600), 1% lead citrate (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... following by a 1-hour incubation in an Aurion Goat Blocking Solution (Aurion, Electron Microscopy Sciences, Fort Washington, PA). The cells were incubated in anti-APOE antibody overnight at 4°C (Rabbit anti-APOE ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Specimen holders were opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed in distilled water by centrifugation and resuspension and then post-fixed in 1% aqueous osmium tetroxide (Electron Microscopy Sciences) for 30 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Neuroscience 2020Quote: ... The EV sample (5μl) was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-s exposure to a glow discharge (25 mA) ...
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bioRxiv - Neuroscience 2021Quote: After 8 washes with 0.1 M cacodylate buffer at pH 7.4 (SigmaAldrich) the tissue was post fixed by 1% osmium tetroxide (Electron Microscopy Sciences) and 0.6% K3Fe(CN)6 in a 0.1 M cacodylate buffer for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... ∼5 × 109 infected RBCs (iRBCs)] and schizont stage (40–46 hpi, ∼1.5 × 109 iRBCs) were harvested and crosslinked with paraformaldehyde (1% final concentration; EMS, USA) at 37°C for 15 min with agitation and then immediately neutralized by adding glycine (0.125 M final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in water and then maleate buffer (MilliporeSigma) (pH=6.0) and stained in 0.05 M Maleate Buffer containing 1% uranyl acetate (Electron Microscopy Sciences) at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2019Quote: ... with a layer of ultra-thin carbon (Agar Scientific) were glow-discharged at 45 mA for 1 min using a K100X Glow Discharge Unit (EMS). A 4 µl-droplet was then applied directly onto the carbon-side of the grid loaded into the humidity chamber of a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... Samples were treated for 1 h with 1% aqueous uranyl acetate and were then dehydrated in a graded ethanol series and embedded in EMBed-812 resin (EMS). After 48 h of polymerization at 60 °C ...
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bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were allowed to dry in open air for 1 hour prior to coverslipping with Cytoseal (Electron Microscopy Sciences #18007).
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bioRxiv - Biochemistry 2019Quote: ... 5µl of the EV sample was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU EMS www.emsdiasum.com) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour and then washed three times for 15 min each with the same buffer before secondary fixation in 1% osmium tetroxide (EMS) prepared in Sorensen’s Phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... 2.5 µl of sample was applied to 300 mesh Quantifoil R 2/1 copper grids (Electron Microscopy Sciences, Hatfield PA) that had been previously glow discharged (15 mA ...
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bioRxiv - Molecular Biology 2024Quote: ... and cover-slipped with ThermoFisher Gold Seal Cover Glass 24 x 60mm No.1 thickness (Electron Microscopy Sciences, 63770-01). Sections were imaged using a Leica CTR6000/B microscope to quantify sulfatide accumulation in different brain areas.
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bioRxiv - Neuroscience 2024Quote: ... Subsequently, sections were incubated in DAPI (1:1000, 10 mins) and mounted with Fluoromount G (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Genetics 2023Quote: AG437 pezo-1(R2405P) early L4 hermaphrodites were washed three times in M9 and soaked in 48 mM Ethyl methane sulfonate (EMS) solution for four hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated in 0.1 M sodium cacodylate buffer supplied with 1% OsO4 and 0.8% potassium ferricyanide (Electron Microscopy Sciences), for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed in water and stained for 1 h at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), and then washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... pieces of tissue were rinsed with 0.1M sodium cacodylate buffer (cacodylate buffer) three times and then postfixed on ice with reduced osmium containing 1% osmium tetroxide (EMS) and 1% ferrocyanide (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Formvar carbon film on 200-mesh copper grids (FCF200-Cu) and uranyl formate (16984-59-1) from Electron Microscopy Sciences were used for sample preparation for negative-stain transmission electron microscopy (TEM).
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed in water and stained for 1 hour at room temperature in 2% uranyl acetate (Electron Microscopy Sciences), washed again in water and dehydrated in a graded series of ethanol ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of culture was sampled (Fig. S1) and chemically fixed by adding paraformaldehyde (PFA; Electron Microscopy Science; EM grade) to a final concentration of 2% ...
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bioRxiv - Bioengineering 2024Quote: ... an overlay of rat tail collagen type 1 solution (2.5 mg/mL) in NF medium (or EMS for disease conditions) was deposited on top of the hepatocytes inside the borders of the oval-well ...
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bioRxiv - Pathology 2024Quote: ... the sections were heated to 650C for 1-2 hours to deparaffinize and treated with Histoclear II (Electron Microscopy Sciences) twice for 15 minutes each ...
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bioRxiv - Neuroscience 2024Quote: ... 500ul 1% neutral red dye in PBS was injected intraperitoneally 2 hours prior to cardiac perfusion with ice cold fixation buffer (2.5% glutaraldehyde, 1% paraformaldehyde, in 0.2 M sodium cacodylate buffer (Electron Microscopy Sciences). Lesion tissues were then dissected out and cut into 2mmx2mm sections ...