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Citations for Electron Microscopy Sciences :
201 - 250 of 260 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the preparations were optically cleared in 25% glycerol for 1 hr at 25 °C and then mounted on Superfrost slides (Cat# 12-550-143, Fisherbrand) with bridged Glass Cover Slips #0 (Cat# 72198-10, Electron Microscopy Sciences) in Fluoromount-G mounting media (Cat# 0100-01 ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on 35-mm Glass Based Dishes which have a cover glass with a grid pattern (IWAKI) and fixed after laser-microirradiation with 4% PFA (Electron Microscopy Sciences) containing 0.1% Triton X-100 (Nacalai Tesque ...
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bioRxiv - Cell Biology 2023Quote: ... The sapphire disks with affixed cells were flat-embedded onto a Teflon-coated glass microscope slide and covered with a Thermanox coverslip (Electron Microscopy Sciences). Resin was polymerized at 60°C for 48 h and the sapphire disks were excised ...
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bioRxiv - Cell Biology 2023Quote: Cells seeded in 24-well plates on poly-D-Lysine-coated glass coverslips were washed once in PBS and fixed with 4 % methanol-free PFA (Electron Microscopy Science) for 15 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The hindlimbs were then submerged in a 100 mL glass beaker filled with 20 mL of Karnovsky fixative [71] ((2.5% EM-grade glutaraldehyde (Electron Microscopy Sciences #16019) plus 2.0% EM-grade paraformaldehyde (Electron Microscopy Sciences #15714-S ...
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bioRxiv - Plant Biology 2023Quote: ... and sections were heat-mounted to glass slides and stained with a diluted toluidine blue solution (catalog no. 14950, Electron Microscopy Sciences).
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bioRxiv - Systems Biology 2022Quote: ... We then bound the chips to a 24×50×1.5mm glass slide (Fisherbrand 12-544-EP) after activating both surfaces for 10s at 100W in a plasma oven (EMS Quorum 1050X). We found that carefully optimizing plasma parameters and controlling humidity were critical for good binding ...
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bioRxiv - Neuroscience 2022Quote: ... while wholemount tissues were mounted under dissecting microscope between slide and glass coverslip using a mounting gel with DAPI (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature (25°C) with 4% PFA (Electron Microscopy Sciences) diluted in PBS and stored at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... snap frozen in OCT blocks and sectioned at a thickness of 7-10 μm and mounted on the center of poly-l-lysine-covered glass slides (63478-AS, Electron Microscopy Sciences). Mouse Whole Embryo Paraffin Sagittal Sections ...
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bioRxiv - Cell Biology 2024Quote: ... The males were starved for 8 hours in empty plastic vials and fed with a 25 mM EMS solution overnight for 16-18 hours (paper tissue soaked with EMS solution on the bottom of the vials). After EMS treatment ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Neuroscience 2022Quote: ... The 40 μm cryosections were cut at −18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences 71314) and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: The cells from the one-step growth experiment were immobilized by spotting 20 μl of fixed cell suspension on SuperFrost Plus glass slides (Electron Microscopy Sciences, USA), on which silicone isolators (Grace Bio-Lab ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal cell number was determined and 1.0-1.25×105 cells were plated on 1 mm glass coverslips (Electron Microscopy Sciences; Cat. #: 72290-03) in a 24-well plate (Falcon ...
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bioRxiv - Cancer Biology 2024Quote: NCI-H295R cells were seeded at 1.5 x 105 cells per cm2 on top of 12 mm diameter poly-L-lysine coated glass coverslips (Electron Microscopy Sciences, Hatfield, PA) placed in wells of a 24-well cell culture plate ...
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bioRxiv - Pathology 2021Quote: ... A replicate plate was fixed for immunofluorescence analysis by adding 32% Paraformaldehyde (Electron Microscopy Sciences) directly to medium to a final concentration of 4% and incubated at room temp for 15 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plates were covered with a Breathe-Easy sealing membrane (Electron Microscopy Sciences, PA, United States) and incubated shaking at 37 °C for 72 h ...
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bioRxiv - Genetics 2023Quote: ... A replicate plate was fixed for immunofluorescence analysis by adding 32% paraformaldehyde (Electron Microscopy Sciences) directly to medium to a final concentration of 4% and incubated at room temp for 15 min ...
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bioRxiv - Neuroscience 2023Quote: NICOs were harvested from culture plates and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) for 4 hours at 4 °C with gentle rocking ...
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bioRxiv - Genomics 2023Quote: ... the cells on plates were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA).
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... a film of germanium (Structure Probe, Inc., West Chester, PA) was applied to glass coverslips of 25 mm diameter and thickness #1 (Electron Microscopy Sciences, Hatfield, PA) as previously described (Heckman et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Whole neural retinas with the photoreceptor layer upside were flattened by performing four symmetrical cuts on Superfrost Plus glass slides (Electron Microscopy Sciences, Hatfield, PA), fixed with 4% PFA for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... the reaction chamber consists of an 18 mm and a 12 mm circular microscope glass slide (Electron Microscopy Sciences, catalogue # 72230-01 & 72222-01) sandwiching two parallel strips of Kapton tape (www.kaptontape.com ...
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bioRxiv - Microbiology 2021Quote: ... in toluene was prepared and spin coated (WS-400B-6NNP/LITE, Laurell Technologies Corporation, North Wales, PA) on to a glass coverslip (25 × 75 mm, Electron Microscopy Sciences, Hatfield, PA) at 50 rpm for 20 sec ...
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bioRxiv - Neuroscience 2021Quote: ... sapphire disks were placed into 12-well plate containing 1 mL of 2% glutaraldehyde (EMS #16530) and 1 mM CaCl2 (Sigma-Aldrich # 63535 ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixated in a 96U plate in 250 µl of 2% paraformaldehyde (PFA, EMS #15710) in PBS at room temperature for 20 min ...
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bioRxiv - Cell Biology 2020Quote: Cells were grown in 96-well plates and fixed by adding 4% PFA (Electron Microscopy Sciences) for 20 mins and then permeabilized with 0.25% Titon-X-100 for 10 mins ...
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bioRxiv - Physiology 2022Quote: ... and then pinned out on a 10cm plate filled with ∼0.5cm of Sylgard (Electron Microscopy Sciences, 24236-10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plates were covered with a Breathe-Easy sealing membrane (Electron Microscopy Sciences, PA, United States) and incubated in a Synergy H1 microplate spectrophotometer (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... plates were aspirated down to 30μL and 30μL of 8% paraformaldehyde (Electron Microscopy Sciences 15714-S) in PBS was added per well ...
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bioRxiv - Cell Biology 2024Quote: ... For TEM cells were fixed (in plates) in 2% paraformaldehyde and 1% glutaraldehyde (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... cleared spinal cords and whole-mount skin samples were mounted on glass slides in 97% TDE using press-to-seal silicone isolators (Electron Microscopy Sciences, Hatfield, PA, USA) with the dorsal surface facing up.
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bioRxiv - Biophysics 2022Quote: Individual worms are picked into a grid of 3μL M9 buffer droplets on glass-printed 4mm hydrophilic spots (Electron Microscopy Sciences Inc. Item #63430-04) surrounded by a hydrophobic PTFE (Teflon ...
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bioRxiv - Neuroscience 2020Quote: ... swirled in 2.5% potassium dichromate and then gently pushed with a wooden toothpick into a glass container containing fresh 0.75% silver nitrate (Electron Microscopy Sciences; Cat# 21050; Hatfield, PA) in HPLC water ...
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bioRxiv - Physiology 2020Quote: ... at −20°C with a thickness of 10um through the mid-belly and mounted on SuperFrost glass slides (Electron Microscopy Sciences, catalog no. 71882-01). For analysis of fiber cross-sectional area (CSA) ...
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bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
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bioRxiv - Neuroscience 2024Quote: ... Poly-l-lysine coated chambers and coverslips were prepared by washing glass microscope slides and glass coverslips with acid alcohol (1% HCl in 70% ethanol) and placing 1:10 0.1% poly-l-lysine (Electron Microscopy Sciences, catalog #19320, Hatfield, PA) in ddH20 on slide for 1 hour before aspirating off and leaving covered in fume hood overnight (Suvarna SK 2013 ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Neuroscience 2021Quote: ... DIV 13 neurons in 24 well plates were fixed with warm 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS for 7 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were mounted using the Fluoromount G mounting medium (ref 17984-25, EMS Diasum, Hatfield, Pennsylvania, USA). For Ki-67 staining ...
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bioRxiv - Microbiology 2021Quote: ... a thin section of the top layer of agar was sliced and placed on a silane-coated glass slide (Electron Microscopy Sciences, Hartford, PA, catalog # 63411-01) with three GeneFrame chambers (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cover slips were mounted on a glass slide with 20 μL of Fluoromount-G containing DAPI (Electron Microscopy Sciences, Hatfield, PA, USA; Cat:17984-24). Confocal microscopy images were obtained using an LSM 700 Zeiss confocal microscope (Carl Zeiss ...
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bioRxiv - Biophysics 2022Quote: ... Samples were removed from 384-well plates and applied to formvar/carbon coated grids (Electron Microscopy Sciences, PA). Samples were stained with 2% uranyl acetate and imaged using a JEOL 1400 Transmission electron microscope with a XR80 camera (AMT imaging ...
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bioRxiv - Microbiology 2022Quote: Cells grown on a coverslip in a 12 well plate were fixed with 4% formaldehyde (Electron Microscopy Sciences) in PBS for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: Embryos were collected on apple juice agar plates and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat. 15710) and permeabilized in 1X PBT ...