Labshake search
Citations for Electron Microscopy Sciences :
201 - 249 of 249 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... a 3-μl aliquot of the protein sample was applied to a glow-discharged Quantifoil holey carbon grid (Au 300 2/1 mesh) (Electron Microscopy Sciences), blotted for 2 s ...
-
bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... protein was diluted to a concentration of 25 nM and applied to CF400-copper ultra-thin support carbon grids (Electron Microscopy Sciences) that were glow discharged with a PELCO easiGlow (Ted Pella ...
-
bioRxiv - Biochemistry 2019Quote: ... centrifuged at 3,000 x g for 5 min and clarified samples incubated for 2 h on carbon-coated Formvar nickel grids (300 mesh, Electron Microscopy Science, Pennsylvania) coated with anti-GFLV polyclonal antibodies (Bioreba AG ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
-
bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
-
bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
-
bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein samples were diluted to 0.05 mg/mL in their respective dialysis buffers and 3.5 μL of the protein samples were applied to freshly glow-discharged formvar-reinforced carbon grids (EMS: FCF-200-AU-EC), washed with water ...
-
bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
-
bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
-
bioRxiv - Immunology 2021Quote: 5 µl of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater, Electron Microscopy Science, Hatfield, PA). Using the Zeiss SmartSEM software ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The vaccine powder was reconstituted and diluted 5 times with water and then transferred to a glow discharge-treated carbon coated copper grid from Electron Microscopy Sciences (Hatfield, PA). The sample was stained with a 2% uranyl acetate solution for 2 min and then air dried ...
-
bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
-
bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
-
bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
-
bioRxiv - Biophysics 2020Quote: ... 5 uL of DNA origami solution (5 nM) was deposited onto fresh glow discharged carbon film with copper grids (CF200H-CU; Electron Microscopy Sciences Inc., Hatfeld, PA), and the sample was then allowed to absorb onto the surface for 30s ...
-
bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
-
bioRxiv - Cell Biology 2022Quote: ... stained en bloc with aqueous 1.0% uranyl acetate for 1 h on ice, (5) progressively dehydrated through an ethanol series (50%, 70%, 90%, and 100%) and 100% propylene oxide (Electron Microscopy Sciences, Hatfield, PA, USA) each for 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used were goat-anti-Rabbit IgG conjugated to 10nm gold particles (Electron Microscopy Sciences, Cat. # 25109). We also labeled the samples with secondary gold-conjugated antibody alone to test for the non-specific labeling and found virtually no labeling in each case (data not shown) ...
-
bioRxiv - Cell Biology 2019Quote: ... All reagents for electron microscopy and secondary antibodies for immuno-gold labelling were purchased from Electron Microscopy Sciences.
-
bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
-
bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
-
bioRxiv - Cancer Biology 2019Quote: ... and surface antibodies were fixed in place by a 15-minute incubation with 1.5% paraformaldehyde (PFA; Electron Microscopy Sciences). Subsequently ...
-
bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Cell Biology 2019Quote: The following reagents/primary antibodies were used for immunofluorescence analyses: paraformaldehyde solution (10%) was purchased from Electron Microscopy Sciences (Hatfield, PA); mouse anti-TfR2 ...
-
bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
-
bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
-
bioRxiv - Developmental Biology 2024Quote: Larvae were dissected to optimally visualize the body wall muscles in ice-cold HL3.1 buffer and fixed with 4% paraformaldehyde in HL3.1 for 20 minutes for all antibodies except GluRIIA for which samples were fixed in Bouin’s fixative (Electron Microscopy Sciences, 15990) for 5 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
-
bioRxiv - Pathology 2022Quote: ... Slides were incubated at RT for an hour in secondary antibody and mounted with Fluoro-Gel II containing DAPI (Electron Microscopy Sciences, 17985). Slides were viewed using a Zeiss Axio Imager.A2 Microscope and images were taken using Axiocam 105 color (chromogenic detection ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...