Labshake search
Citations for Electron Microscopy Sciences :
201 - 250 of 1240 citations for Mono 2 Ethyl 5 Hydroxyhexyl Phthalate 13C4 99% Dehp Metabolite Ix 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
-
bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
-
bioRxiv - Genomics 2021Quote: ... and the cells were fixed with 2 ml of 4% PFA in PBS (Electron Microscopy Sciences, 15710) for 20 min at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Biophysics 2019Quote: 5 uL of pCV2-2 VLP samples was applied to CF200-CU carbon film 200 mesh copper grids (Electron Microscopy Science) for 1 min and the grids were then washed with 200 µl of 50 mM of Na Cacodylate buffer and then strained immediately with 50 µl of 0.5% uranyl acetate for 1 min ...
-
bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low- barium PBS for 5 min ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were washed with acetone and infiltrated with increasing concentrations (25, 50, 75 and 100%; 2 hours each) of EPON resin (Electron Microscopy Sciences). 100% EPON solution was exchanged two times in 12-hour steps ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
-
Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
-
bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
-
bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
-
bioRxiv - Biochemistry 2019Quote: ... centrifuged at 3,000 x g for 5 min and clarified samples incubated for 2 h on carbon-coated Formvar nickel grids (300 mesh, Electron Microscopy Science, Pennsylvania) coated with anti-GFLV polyclonal antibodies (Bioreba AG ...
-
bioRxiv - Neuroscience 2020Quote: ... We next dehydrated the samples using chilled ethanol-water mixtures (70 %, 100 %, 100 %, 100 %, ethanol (Electron Microscopy Sciences), 10 ...
-
bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
-
bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
-
bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
-
bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
-
bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL of the infected and control cultures were fixed in paraformaldehyde (Electron Microscopy Sciences, ref. 15714, 1% final conc.) and glutaraldehyde (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
-
bioRxiv - Immunology 2019Quote: ... prior to deposition onto 2/2 Quantifoil grids (EMS) that were glow discharged for 10 s directly preceding the deposition in a Vitrobot (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... tissue was dehydrated through an ethanol series (70% ethanol, 90% ethanol, 2 x 100% ethanol; 8 minutes each) and mounted in Entellan (Electron Microscopy Science, Hatfield, PA, USA).
-
bioRxiv - Genomics 2019Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
-
bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
-
bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Neuroscience 2021Quote: ... infiltrated in 1:1 volumes of 100% ethanol and 100% LR White (EMS) during 4 h ...
-
bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
-
bioRxiv - Microbiology 2021Quote: ... samples were dehydrated through a graded ethanol series (20%, 40%, 60%, 80%, 90%, 100%, 100%, 100%) followed by infiltration with an Embed-812 epoxy resin (Electron Microscopy Sciences):acetone series of 1:3 for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were dehydrated in a series of graded (25, 50, 75, 100, 100, 100 v/v%, 10 min each) acetonitrile (Electron Microscopy Sciences) aqueous solutions ...
-
bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer (EMS) ...
-
bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS), 2.2mM CaCl2 diluted in 50 mM MOPS buffer (pH 7.4 ...
-
bioRxiv - Pathology 2021Quote: ... slides were washed twice with 1 mL of PBS wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were first fixed with 1 mL of the fixative (2% paraformaldehyde [Electron Microscopy Sciences] dissolved in 250 mM HEPES [pH 7.4]) for 10 min at room temperature (approximately 25°C) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 100% and 100% Hexamethyldisilazane (v/v in ethanol) (HMDS, Electron Microscopy Sciences, Cat# 16700) for 30 min per step at RT ...
-
bioRxiv - Biochemistry 2024Quote: Quanifoil R 2/2 200 mesh copper grids (EMS #Q2100-CR2) were glow discharged at 25mA for 25s using a Pelco easiGlow system (Ted Pella) ...
-
bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Neuroscience 2022Quote: ... Fibril assemblies were next analyzed via transmission electron microscopy at an applied concentration of 0.4 mg per mL, with fibrils absorbed to glow-discharged 300 mesh (Ladd Research Industries, Inc.) and stained with 2% uranyl acetate (Electron Microscopy Sciences, Inc.) in water ...
-
bioRxiv - Neuroscience 2021Quote: ... A small piece (∼2 × 2 mm) containing V1 was cut out and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in Sodium Cacodylate buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...