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Citations for Electron Microscopy Sciences :
201 - 250 of 3154 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... the flame sealed end of the tubing was immediately dipped into a parlodion solution (∼3 cm2 parlodion, Electron Microscopy Sciences [Hatfield ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 4% PFA (1:4 diluted with 1× PBS from Paraformaldehyde 16% Aqueous Solution, Electron Microscopy Sciences) to pre-fix the brain ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2024Quote: Clinical samples and biofilm samples fixed using the SEM fixation buffer were subsequently subjected to a series of washing steps (3 repetitions for 5 mins each) with 0.1M Cacodylate buffer pH 7.4(Electron Microscopy Sciences, USA) and distilled water (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL sample was applied to glow discharged Quantifoil® R1.2/1.3 Cu 300 mesh grid (Electron Microscopy Sciences, PA, USA). The samples were then plunged into liquid ethane using a Vitrobot™ Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Copper grids were post stained with a 1.2% UA solution and a 3% lead citrate solution (Electron Microscopy Sciences Cat#22410) for 4 min each ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... zmcd1-1 and zmcd1-2 (EMS mutants), as well as the F1 hybrids (obtained by crossing the EMS mutants with test inbred lines ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were fixed in 4% paraformaldehyde (a 1:1 mixture of 8% paraformaldehyde, Electron Microscopy Sciences (EMS) 157-8 ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were fixed in 4% paraformaldehyde (a 1:1 mixture of 8% paraformaldehyde, Electron Microscopy Sciences (EMS) 157-8 ...