Labshake search
Citations for Electron Microscopy Sciences :
2401 - 2450 of 3233 citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The sample was double-stained with 6 µl of 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) for 5 seconds (first stain ...
-
bioRxiv - Immunology 2022Quote: ... a 2 µl aliquot of EV preparation was placed onto a carbon film grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 1 minute ...
-
bioRxiv - Synthetic Biology 2020Quote: ... was adsorbed onto formvar-carbon coated copper grids for 2 min and negatively stained with uranyl acetate replacement stain (UAR-EMS) for 1 h ...
-
bioRxiv - Bioengineering 2019Quote: ... agarose RoCs with ROs and RPE were fixed in the chambers with Karnovsky buffer (2.5% glutaraldehyde, 2% paraformaldehyde, 0.1 M sodium cacodylate buffer, pH 7.4) (Electron Microscopy Sciences, Germany) for 12 hours at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... The medial shell of the NAc and a piece of tissue spanning the entire VTA were excised and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was quickly extracted and cut into 2 mm coronal slices using a rodent brain matrix (Electron Microscopy Sciences). Slices were placed on ice cold PBS and PFC and VCX were further dissected and collected for RNA extraction ...
-
bioRxiv - Biophysics 2020Quote: ... 270 µL of GPMV solution was pipetted from the bottom of the Eppendorf tube and sandwiched between 2 coverslips coated with 0.1% BSA and separated by a 0.5 mm thick silicone isolator (Electron Microscopy Sciences).
-
bioRxiv - Cell Biology 2022Quote: Isolated mouse islets and MIN6 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer (cat. no. 15960-01 Electron Microscopy Sciences) for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat ...
-
bioRxiv - Cell Biology 2024Quote: ... and fixed by immersion in Modified Karnovsky’s Fixative (2% Paraformaldehyde and 2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4 (Electron Microscopy Sciences (Hatfield, PA), #15960-01) ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were fixed in 0.1 M cacodylate buffer (pH 7.4) with 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and 2% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Neuroscience 2023Quote: ... The coverslips were then mounted with DAPI (diamidino-2-phenylindole)-free Fluoro-Gel on glass slides (Electron Microscopy Sciences, USA). Cells were scanned using a 63x oil immersion objective mounted on a confocal microscope (LSM 710 Meta ...
-
bioRxiv - Genetics 2023Quote: ... the supernatant was discarded and the pellet resuspended in primary fixating buffer (2% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4, Electron Microscopy Sciences) for 30 min at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: Immunofluorescence assay was performed on PHA-E-immobilised parasites by fixation with 2% paraformaldehyde and 0.008% glutaraldehyde (Electron Microscopy Sciences) and permeabilisation with 0.1% Triton X-100 in PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed with glutaraldehyde 2.5% in 0.1 M sodium cacodylate buffer supplemented with 2 mM of calcium chloride (Electron Microscopy Sciences), post-fixed with 1% OsO4 and 1.5% potassium ferrocyanide in sodium cacodylate buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells on nanostructures were washed with PBS and fixed in 0.1 M sodium cacodylate buffer (pH 7.2) supplemented with 2% glutaraldehyde (Electron Microscopy Sciences) and 4% paraformaldehyde (PFA ...
-
bioRxiv - Plant Biology 2023Quote: ... and the resulting cell pellets were fixed using a mixture of 2% [v/v] paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and 2% [v/v] glutaraldehyde (Agar Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... They were subsequently transcardially perfused with 15 ml of 0.1M sodium phosphate buffer pH 7.4 and then 60 ml of 2% paraformaldehyde (15710-S, Electron Microscopy Sciences) 1% glutaraldehyde (G7526 ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
-
bioRxiv - Microbiology 2023Quote: Genomic libraries were constructed and sequenced by MiSeq using paired-end sequencing (2 × 300 bp) for 17 strains generated by EMS mutagenesis and one strain ...
-
bioRxiv - Biochemistry 2024Quote: Prom1 and Ttyh1 EVs were vitrified on 300-mesh gold Quantifoil R 1.2/1.3 + 2 nm Carbon grids (Electron Microscopy Sciences). Briefly ...
-
bioRxiv - Neuroscience 2024Quote: ... en bloc staining protocol (Kislinger et al., 2020a) (Kislinger et al., 2023) including post-fixation in 2% osmium tetroxide (EMS), 1.5% potassium ferrocyanide (Sigma ...
-
bioRxiv - Genetics 2024Quote: ... The coverslip was mounted onto a concave microscope slide to reduce media evaporation (2-Well Concavity Slide, Electron Microscopy Sciences). Images were acquired using an AX70 Olympus epifluorescence microscope across 11 z-planes with 0.5 µm of interplanar distance ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains from adult females were dissected in Phosphate-buffered saline (PBS, Eurobio, ref CS0PBS0108) and fixed for 15 min in 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15714-S) at room temperature (RT) ...
-
bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
-
bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
-
bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
-
bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
-
bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... were incubated with 8 ml of 0.74 % ethyl methanesulfonate (EMS, Sigma) in 0.1 M NaH2PO4 ...
-
bioRxiv - Molecular Biology 2021Quote: BF PolIE RNAi cells were grown with or without doxycycline for 24 hours then incubated with 2 mM of ethylmethanesulfonate (EMS; Sigma) or DMSO (vehicle ...
-
bioRxiv - Zoology 2021Quote: Sets of oocytes including some number of visibly infected individuals were fixed in 2% glutaraldehyde in 0.2 M sodium cacodylate buffer (SCB, Electron Microscopy Sciences) for one hour and post-fixed in 2% osmium tetroxide in SCB for one hour (Bozzola and Russell ...
-
bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
-
bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on bone slices for 3 days were unroofed as described above and fixed for 10 min in a 0.2M sodium cacodylate buffer (pH 7.4) containing 2% paraformaldehyde (Electron Microscopy Sciences 15710) and 2.5% glutaraldehyde (Electron Microscopy Sciences 16220) ...
-
bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μl of diluted sample was deposited onto a glow-discharged (Edwards S150B, 30sec, 30-50 mA, 1,2kV, 10-2 mbar) continuous carbon grid (CF400-CU-UL, Electron Microscopy Sciences). After one minute at RT ...
-
bioRxiv - Biophysics 2022Quote: ... then cells were allowed to adhere for 15 min in media prior to chemical fixation in 2% PFA and 0.2% glutaraldehyde (Electron Microscopy Sciences). FActin was stained by permeabilizing cells with 0.1% Triton-X-100 prior to incubation with 3.3 μM phalloidin-AlexaFluor647 (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: Glass vials were prepared prior to the experiments with a 2-mm layer of Sylgard 184 (Electron Microscopy Sciences, Hatfield, PA) at the bottom ...
-
bioRxiv - Neuroscience 2019Quote: 63 day old hMO specimens were immersion-fixed in a solution of 2 % PFA and 2.5 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences, Hatfield, PA) for 3 h ...
-
bioRxiv - Bioengineering 2020Quote: ... bone pieces were washed with double distilled water for 2 minutes and embedded in Epon epoxy resin dissolved in acetone (Embed 812, EMS, USA). The Epon embedding was carried out in 5 steps ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... atala eye was immersed for prefixation in 2.5% Glutaraldehyde/2% paraformaldehyde in 0.1M Sodium Cacodylate buffer (pH 7.4) (Electron Microscopy Sciences, PA, USA) for 2h at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Copper grids containing thin sections were post-stained for 20 min in 2% aqueous uranyl acetate (Electron Microscopy Sciences; Cat #22400) and for 5 min in Reynolds lead citrate (Reynolds ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were transfer into slicksealTM microcentrifuge tube (National Scientific, CA170S-BP) and fixed with 2% paraformaldehyde (Electron Microscopy Science, Nm15710) in phosphate-buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 ul chromatin samples with a concentration of about 0.2 mg/ml DNA were applied to Quantifoil R2/2 200 mesh copper grids (EMS Q250-CR2). Vitrification was conducted by plunging into liquid ethane using our FEI Vitrobot Mk IV Grid Plunging System at 100% humidity ...
-
bioRxiv - Cancer Biology 2022Quote: ... sample coverslips were washed 2 times in staining buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... P21 and P28) were deeply anesthetized and perfused with 0.9% saline followed by 2% paraformaldehyde (PFA) and 2.5% glutaraldehyde (EMS, Hatfield, PA, USA) in 0.1 M phosphate buffer (PB) ...
-
bioRxiv - Cell Biology 2024Quote: ... sample slides were washed 2 times in Staining Buffer and fixed for 10 minutes in 1.6% paraformaldehyde (Electron Microscopy Sciences, 15710) in storage buffer ...