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Citations for Electron Microscopy Sciences :
2351 - 2400 of 2473 citations for 7 Hydroxy 4 methoxy 1 indanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... sections were cut with an ultra 45° Diatome™ diamond knife on a Leica Ultracut UCT microtome and collected on formvar support film slots 2×1 mm cu grids (EMS). Sections were counterstained with 2% ethanolic uranyl acetate ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of each sample (∼1 mg/mL) was applied to a freshly glow discharged (15mA, 1 min) holey carbon copper grid (apo-37C: Cflat (EMS) R1.2/1.3 ...
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bioRxiv - Biophysics 2024Quote: ... 2.5% glycerol) was applied to glow discharged gold grids (Au-flat, 0.6 µm/1 µm, 300 mesh, Electron Microscopy Sciences). The sample was blotted with the minimum blot time that can be set (0 s) ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2024Quote: ... Sapphire discs with attached frozen hTERT-RPE-1 cells and type-A planchettes containing worms were transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resin infiltrated in ethyl alcohol and Spurr’s resin epoxy mixtures using an automated EMS Lynx 1 EM tissue processor (Electron Microscopy Sciences). Processed samples were infiltrated with two changes of fresh Spurr’s resin for 24 hours and the samples were then oriented in the resin ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Systems Biology 2020Quote: ... rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Systems Biology 2020Quote: ... the La2-DAB solution was removed and cells were rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen samples were then opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS, USA) and 0.1% (w/v ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Hippocampi from 6-month-old PS19 mouse brains with and without 3-month glutamine administration were cut into small specimens (one dimension < 1 mm) and fixed in Trump’s fixative (Electron Microscopy Sciences) for 2 hr at RT ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Developmental Biology 2023Quote: ... infiltrated with Embed-812 resin series by 20% increments in acetone (each step was 1 h) and embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60°C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Bioengineering 2023Quote: ... A 3-μL droplet of the L/D-GQD solution (0.1 mg·mL-1) was placed on the carbon-coated copper TEM grid (CF200-Cu-25; Purchased from Electron Microscopy Sciences, PA) and allowed to dry in the air ...
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
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bioRxiv - Biophysics 2024Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Bioengineering 2024Quote: A 20 µL droplet of 10 nM origami solution diluted in 1×TAE buffer sup- plemented with 12.5 mM MgCl2 was deposited onto a freshly cleaved mica substrate (Electron Microscopy Sciences). The sample was incubated for 1 minute ...
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bioRxiv - Microbiology 2024Quote: 3 µl of sheath preparation sample was incubated for 1 min on glow-discharged carbon-coated formvar grids (Electron Microscopy Sciences). To stain the sample ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was added to immobilize rosettes (300 µL of a 1:2,000 dilution of a 16% PFA stock solution; Electron Microscopy Sciences 15714 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS twice followed by fixation and permeablization in 1 ml PBS containing 2% PFA (Electron Microscopy Sciences) and 0.1% saponin (Sigma–Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were mixed with 0.5 µl of 0.04 mM Lauryl maltose neopentyl glycol (LMNG) solution immediately before sample deposition onto QUANTIFOIL® 2/1 400 mesh grids (EMS), which were plasma cleaned for 10 s using Gatan Solarus 950 Plasma system (Ar/O2 gas mixture) ...
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bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Biophysics 2021Quote: ... 2uL of dialyzed sample was then applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 200- or 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were subsequently blotted for 2 or 3 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...