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Citations for Electron Microscopy Sciences :
2251 - 2300 of 2438 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Samples were then post-fixed with 1% glutaraldehyde in 0.15 M Sorensen’s phosphate buffer (SPB; Electron Microscopy Sciences) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in ddH2O and incubated on ice for 1 hour in a solution composed of 1.5% potassium ferrocyanide and 1.5% osmium tetroxide (EMS) diluted in ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged and the pellet was resuspended with PBS containing 1% formaldehyde (Electron Microscopy Sciences, cat# 15714) for further crosslinking ...
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bioRxiv - Neuroscience 2021Quote: ... The antigen retrieval was performed in 1 x Citrate buffer pH 6.0 in ddH2O (Electron Microscopy Sciences, 64142-08). 1 x Citrate buffer pH 6.0 in ddH2O was warmed up to 95°C and applied over samples for 20 min and let it cool at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... This was polymerised for 24 h at 60°C inside a closed gelatin capsule (size 1; Electron Microscopy Sciences) half-filled with previously hardened resin to ensure the insect was situated in the center of the final resin block ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) for 1h at room temperature (RT) and post-fixed in a fresh mixture of 1% osmium tetroxide (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 2.5% in phosphate buffer (PB 0.1 M [pH 7.4]) for 1h at RT and post fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were washed with ddH2O at room temperature and then placed in the 0.22 µm-Millipore-filtered 1% thiocarbohydrazide (TCH) (#21900 EMS) in ddH2O solution for 20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: Fresh pancreas samples were cut into 1 mm fragments and immersion fixed in a modified Karnovsky’s fixative (51) (2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Cell Biology 2019Quote: ... samples of 1-2 mm thickness were fixed through immersion in 5% (w/v) glutaraldehyde (Biological Grade, EMS, #16500) in 0.05M phosphate buffer pH 7.3 for 24 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Tendons of interest were identified by collecting 1 μm sections stained with an epoxy tissue stain (Electron Microscopy Sciences). Ultrathin sections containing tendons of interest were cut at 80 nm ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was immersed for 5 min in reduced osmium solution containing 1% osmium tetroxide (OsO4; Electron Microscopy Sciences) and 1.5% potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with 10 nm gold-conjugated goat anti-guinea pig IgG (1:200, Electron Microscopy Sciences, #25329) in blocking buffer for 3 hr ...
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bioRxiv - Cell Biology 2022Quote: ... four to five serial sections were picked up per 2 × 1 mm formvar and carbon coated slot grid (EMS). Because the Aclar coverslip ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then rinsed with 0.1 M cacodylate buffer and fixed with 1 % osmium tetroxide (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at room temperature ...
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bioRxiv - Physiology 2023Quote: ... One microliter of this resuspension is added to 1 mL of Mammalian Ringer’s Solution (Electron Microscopy Sciences #11763-10) containing isolated myofibers from the flexor digitorum brevis muscle (FDB ...
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bioRxiv - Biophysics 2023Quote: ... MMA/graphene was applied onto 300 mesh gold-coated Quantifoil R 0.6/1 copper grids (Electron Microscopy Sciences, USA) by using the grids to scoop out the bilayer ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for SEM imaging by OTOT method where two consecutive baths of buffered 1% osmium tetroxide (EMS) and 1% tannic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and allowed to completely dry before staining with either a Sato’s Lead solution (1% lead acetate (EMS, Cat# 17600), 1% lead citrate (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... following by a 1-hour incubation in an Aurion Goat Blocking Solution (Aurion, Electron Microscopy Sciences, Fort Washington, PA). The cells were incubated in anti-APOE antibody overnight at 4°C (Rabbit anti-APOE ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Specimen holders were opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed in distilled water by centrifugation and resuspension and then post-fixed in 1% aqueous osmium tetroxide (Electron Microscopy Sciences) for 30 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Neuroscience 2020Quote: ... The EV sample (5μl) was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-s exposure to a glow discharge (25 mA) ...
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bioRxiv - Biochemistry 2021Quote: ... and 3 µL of sample was applied onto glow-discharged C-flat grids (EMS CF-1.2/1.3-4Au-1. 50) for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: After 8 washes with 0.1 M cacodylate buffer at pH 7.4 (SigmaAldrich) the tissue was post fixed by 1% osmium tetroxide (Electron Microscopy Sciences) and 0.6% K3Fe(CN)6 in a 0.1 M cacodylate buffer for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... ∼5 × 109 infected RBCs (iRBCs)] and schizont stage (40–46 hpi, ∼1.5 × 109 iRBCs) were harvested and crosslinked with paraformaldehyde (1% final concentration; EMS, USA) at 37°C for 15 min with agitation and then immediately neutralized by adding glycine (0.125 M final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in water and then maleate buffer (MilliporeSigma) (pH=6.0) and stained in 0.05 M Maleate Buffer containing 1% uranyl acetate (Electron Microscopy Sciences) at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... A 3.5 μL sample containing the complex (1 mg/ml) was applied to a glow-discharged UltraAuFoil grid (R1.2/1.3, mesh 300) (Electron Microscopy Sciences), and then blotted and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2019Quote: ... with a layer of ultra-thin carbon (Agar Scientific) were glow-discharged at 45 mA for 1 min using a K100X Glow Discharge Unit (EMS). A 4 µl-droplet was then applied directly onto the carbon-side of the grid loaded into the humidity chamber of a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: Three microliters of the Ric-8A:ΔN31Gαi1:4Nb complex at 0.4 mg/ml with 0.01% NP40 were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (Electron Microscopy Sciences). Grids were blotted for 4 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... Samples were treated for 1 h with 1% aqueous uranyl acetate and were then dehydrated in a graded ethanol series and embedded in EMBed-812 resin (EMS). After 48 h of polymerization at 60 °C ...
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bioRxiv - Microbiology 2019Quote: Negative stain specimens were prepared by applying 4 µL of protein solution (0.1 mg/mL + 1 mM AMP-PnP) onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS). After 30 s the protein solution was blotted with a piece of filter paper ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were allowed to dry in open air for 1 hour prior to coverslipping with Cytoseal (Electron Microscopy Sciences #18007).
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bioRxiv - Biochemistry 2019Quote: ... 5µl of the EV sample was adsorbed for 1 min to a carbon-coated mesh grid (# CF400-CU EMS www.emsdiasum.com) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... for one hour and then washed three times for 15 min each with the same buffer before secondary fixation in 1% osmium tetroxide (EMS) prepared in Sorensen’s Phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Physiology 2022Quote: ... they were rinsed 3 times 5 minutes in PB buffer and then postfixed by a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... We then washed samples (2 × 30 minutes in H2O) and transferred samples into 1% TCH (#21900; EMS, Hatfield, PA, USA) in H2O for 45 minutes at 40°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed 3 × 5 min in PB and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% potassium ferrocyanide in PB buffer for 2 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... pieces of tissue were rinsed with 0.1M sodium cacodylate buffer (cacodylate buffer) three times and then postfixed on ice with reduced osmium containing 1% osmium tetroxide (EMS) and 1% ferrocyanide (Fisher Scientific ...