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Citations for Electron Microscopy Sciences :
2201 - 2250 of 2575 citations for Methyl 3 2 morpholin 4 ylethoxy benzoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown on 35 mm Glass Based Dishes or 24-well EZVIEW Glass Bottom Culture Plates LB (IWAKI) and fixed with 4% PFA (Electron Microscopy Sciences) for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen (invadopodia formation assay) or gelatin-coated coverslips (degradation assay) and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue-HDMI-array sandwich was moved to room temperature, and the tissues were fixed in 4% formaldehyde (100 μl, diluted from the EM-grade 16% paraformaldehyde (#15170, Electron Microscopy Sciences)) for 10 min.
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Physiology 2022Quote: ... Adult fish were cardiacally perfused with a solution of 4% paraformaldehyde (PFA) diluted in phosphate buffered saline (PBS) solution (Electron Microscopy Sciences) to remove autofluorescence from blood cells40 ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2021Quote: At the end of experiments mice were perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science, USA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mm diameter PDMS wells were passively adhered to #1 9 mm x 9 mm glass coverslips (Electron Microscopy Sciences #72190-09). Wells were passivated overnight using vapor deposition of Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931 ...
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: Neurons were rinsed 1X with PBS at room temperature and then fixed for 15 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Sciences) made up in PBS and supplemented with 4% sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... plated in glass bottom dishes or hESC-derived striatal neurons on coverslips were washed in D-PBS and fixed for 10 min in 4% PFA (Electron Microscopy Sciences). The cells were washed 3 times with D-PBS and incubated with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Physiology 2021Quote: ... whole heads or enucleated eyes were fixed for 20 minutes on ice in 4% PFA prepared in PBS from 20% stock solution (15713, Electron Microscopy Sciences). The samples were rinsed with PBS and stored in 100% methanol at −20°C ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).
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bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS, 69039-60). The silicon chamber was mounted on a 1.5-mm clean cover glass (EMS ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The animals were then transcardially perfused with PBS and then with 4% paraformaldehyde (PFA) (diluted into 1xPBS from 16% PFA, Electron Microscopy Sciences), followed by post-fixation with 4% PFA for immunohistochemistry or Karlsson and Schultz (KS ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Neuroscience 2024Quote: Mice were placed under isoflurane anesthetics until loss of pain reflex and transcardially perfused with filtered ice-cold PBS then 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were cryoprotected in 30% sucrose ...
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bioRxiv - Neuroscience 2024Quote: P14 mice were euthanized and perfused transcardially with ice-cold Phosphate Buffered Saline (PBS) followed by ice-cold 4% paraformaldehyde (Electron Microscopy Sciences) diluted in water ...
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: Mice were deeply anesthetized with a mixture of Ketamine/Xylazine and perfused transcardially with saline followed by 4% paraformaldehyde (PFA; Electron Microscopy Science). Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on 35-mm Glass Based Dishes which have a cover glass with a grid pattern (IWAKI) and fixed after laser-microirradiation with 4% PFA (Electron Microscopy Sciences) containing 0.1% Triton X-100 (Nacalai Tesque ...
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bioRxiv - Biochemistry 2024Quote: To generate negative-stain grids 4 µl of the GraFix fraction was applied to a Copper 400 Mesh grids with continuous carbon (Electron Microscopy Sciences) after glow discharge ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Developmental Biology 2023Quote: ... Adherent cells (at ESC stage for the CRISPRa validations and PP stage for the CRISPRi validations) were rinsed once with PBS with Ca2+ and Mg2+ (PBS++) and fixed with 4% Paraformaldehyde (Electron Microscopy Sciences 32% Paraformaldehyde ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice in warm 1X HBSS and fixed for 15min in 4% paraformaldehyde (PFA) made from fresh 16% PFA (#15710, Electron Microscopy Sciences) diluted with 1X HBSS ...
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bioRxiv - Bioengineering 2023Quote: Rats implanted with MEV probes were anaesthetized with 3% isoflurane or 300 mg kg−1 of ketamine (Patterson Veterinary Supply) and transcardially perfused with ice-cold 30 ml saline and 30 ml 4% paraformaldehyde (PFA, Electron Microscopy Sciences) at specified times post-injection ...
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bioRxiv - Neuroscience 2023Quote: ... the monkeys were deeply anesthetized (ketamine, 10 mg/kg and sodium pentobarbital, 100 mg/kg) and transcardially perfused with 4% formaldehyde (EMS, Hatfield, PA) in Phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...