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Citations for Electron Microscopy Sciences :
2201 - 2250 of 2460 citations for 6 Fluoro 2 methylamino 4 1H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence analysis of PGCLC bodies or rOvaries was performed on cryosections prepared as follows: aggregates were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science, 15713) in PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to a scintillation vial containing a 1:1 (v/v) mixture of heptane and 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) in PBS on a shaker for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with 20 mL ice cold 1X DPBS followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA). Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... whole-mount preparations of the lateral wall of lateral ventricles of four animals per group were dissected and fixed with 2 % PFA + 2.5 % glutaraldehyde (EMS) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2022Quote: ... perfused in toto (PBS; 10mL) and fixed (2% formaldehyde EMS #15710, 2.5% glutaraldehyde EMS #16220 in 0.1M Phosphate Buffer). Kidneys were harvested and fixed in the same solution ...
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bioRxiv - Cancer Biology 2019Quote: Fresh pancreas samples were cut into 1 mm fragments and immersion fixed in a modified Karnovsky’s fixative (51) (2% paraformaldehyde (EMS), 2.5% glutaraldehyde (EMS) ...
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bioRxiv - Biophysics 2021Quote: ... and permeabilized for 2 min with 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and Thermo Fisher, 28314) in pre-warmed (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Brain slices were washed extensively in cacodylate buffer at room temperature and stained sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Cell Biology 2019Quote: ... samples of 1-2 mm thickness were fixed through immersion in 5% (w/v) glutaraldehyde (Biological Grade, EMS, #16500) in 0.05M phosphate buffer pH 7.3 for 24 hours ...
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bioRxiv - Bioengineering 2019Quote: Samples of the PG-Gly-PL NBs washed with PBS after isolation at concentrations around 1010 particles were applied to Quantifoil holey carbon EM grids (R2/2, 400 mesh:EMS). The EM grids were glow discharged for 30 sec at 15 mA before the sample was applied ...
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bioRxiv - Microbiology 2021Quote: ... the grids were washed with PBS three times before being fixed with 2% EM grade paraformaldehyde (EMS, EM Grade) for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Molecular Biology 2021Quote: Eyes were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Etched coverslips were cleaned of oil and fixed in a 2% solution of glutaraldehyde in PBS (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2022Quote: ... before being fixed by perfusion using 0.15M cacodylate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences) as well as 2mM CaCl2 at 35°C for 5 minutes ...