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Citations for Electron Microscopy Sciences :
2201 - 2250 of 3000 citations for 2E 4E 2 4 Octadien 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by intraperitoneal injection of 100 mg/kg ketamine /20 mg/kg xylazine (Anased) mix and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences 50980487). Brains were dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were initially washed with 1X Assay buffer (GOLGI-ID kit) and then fixed with 4% of 16% paraformaldehyde solution (Electron Microscopy Sciences, 15710) at room temperature for 10 minutes before staining the cells for detection of Golgi bodies using the manufacture’s manual for “Staining Adherent Fixed Cells” ...
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bioRxiv - Neuroscience 2024Quote: ... the 33 mice were deeply anesthetized with isoflurane and transcardially perfused with 10mL of ice-cold PBS followed by 10mL of 4% paraformaldehyde (16% PFA diluted in PBS; Electron Microscopy Sciences 15710). The olfactory bulbs (OBs ...
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bioRxiv - Neuroscience 2024Quote: At 10-15 days in vitro (DIV) coverslips with cells were washed with phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde (PARA) (Electron Microscopy Sciences, ON) in PBS for 10-15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the tops of the wells were cleaned and cells on the bottom of the membrane were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, 15713) and stained with 10 μg/mL DAPI in 1% Triton X100 (Sigma T9284) ...
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bioRxiv - Developmental Biology 2024Quote: Bovine embryos and embryonic cells from different batches/days of experiments were fixed in freshly made 4% paraformaldehyde (Electron Microscopy Science, Hatfield, PA) at room temperature for 15 minutes followed by permeabilization in 1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... whole-mount preparations of the lateral wall of lateral ventricles of four animals per group were dissected and fixed with 2 % PFA + 2.5 % glutaraldehyde (EMS) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2022Quote: ... perfused in toto (PBS; 10mL) and fixed (2% formaldehyde EMS #15710, 2.5% glutaraldehyde EMS #16220 in 0.1M Phosphate Buffer). Kidneys were harvested and fixed in the same solution ...
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bioRxiv - Biophysics 2021Quote: ... and permeabilized for 2 min with 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and Thermo Fisher, 28314) in pre-warmed (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Brain slices were washed extensively in cacodylate buffer at room temperature and stained sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... the grids were washed with PBS three times before being fixed with 2% EM grade paraformaldehyde (EMS, EM Grade) for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Molecular Biology 2021Quote: Eyes were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Etched coverslips were cleaned of oil and fixed in a 2% solution of glutaraldehyde in PBS (Electron Microscopy Sciences).
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bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were then stained with a 2% (w/v) solution of uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). High-resolution TEM images were captured using a JEOL JEM-2010 TEM and JEOL JEM-2100 Field Emission Gun TEM ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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bioRxiv - Cell Biology 2022Quote: ... placed on 200 mesh copper TEM grids and were stained with 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) and Reynold’s lead citrate (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... followed by embedding in 2% agarose and post-fixed in 0.1 M osmium tetroxide (SKU 19152, Electron Microscopy Science). Dehydration was achieved through a series of acetone solutions with increasing concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... before being fixed by perfusion using 0.15M cacodylate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences) as well as 2mM CaCl2 at 35°C for 5 minutes ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl) and dried overnight on Formvar/Carbon-coated hexagonal copper mesh grids (FCF200H-CU-TB, Electron Microscopy Sciences). The grids were observed on a transmission electron microscope JEM 1010 (Jeol ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were stained for 25 seconds with a 2% aqueous solution of uranyl acetate from Electron Microscopy Sciences. Following staining ...
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bioRxiv - Microbiology 2024Quote: ... Clarified cell supernatant was then fixed with 2% PFA (10mL of 8% EM grade PFA, Electron Microscopy Science, USA). The fixed viral particles were then purified by ultra-centrifugation at 124000g for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... one set of control coverslips was washed with PBS before being fixed in 2% PFA (Electron Microscopy Science, 15710) for 15 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... with 0.5% uranyl acetate (Uranyl acetate solution 1%, Electron Microscopy Sciences, cat.#22400-1) and 3% lead citrate (Ultrostain 2 ...
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bioRxiv - Neuroscience 2023Quote: ... 3% water and 1% anhydrous methanol containing 1% uranyl acetate (Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Neuroscience 2023Quote: ... 3% water and 1% anhydrous methanol containing 1% uranyl acetate (Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Genomics 2023Quote: ... a minimum of 1 million ESCs were fixed with 1% Formaldehyde (Electron Microscopy Sciences) for 10 min on a shaker at room temperature and quenched with Glycine 0.125M for 5 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Preinfiltration was performed in a 1:1 mixture of ethanol:Technovit 7100 (Electron Microscopy Sciences) for 4 h under light agitation ...