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Citations for Electron Microscopy Sciences :
2151 - 2200 of 2364 citations for N Nitrosodiphenylamine 2 2 4 4 6 6 D6 96% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... placed on 200 mesh copper TEM grids and were stained with 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) and Reynold’s lead citrate (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... followed by embedding in 2% agarose and post-fixed in 0.1 M osmium tetroxide (SKU 19152, Electron Microscopy Science). Dehydration was achieved through a series of acetone solutions with increasing concentrations ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were then stained with a 2% (w/v) solution of uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). High-resolution TEM images were captured using a JEOL JEM-2010 TEM and JEOL JEM-2100 Field Emission Gun TEM ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were stained for 25 seconds with a 2% aqueous solution of uranyl acetate from Electron Microscopy Sciences. Following staining ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl) and dried overnight on Formvar/Carbon-coated hexagonal copper mesh grids (FCF200H-CU-TB, Electron Microscopy Sciences). The grids were observed on a transmission electron microscope JEM 1010 (Jeol ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Mounting media (~150 μL/slide) was prepared the day of use by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Developmental Biology 2019Quote: Mice were sacrificed according to ethical guidelines and inner ears were dissected out in cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were subjected to intracardiac perfusion with 5mL of PBS containing heparin followed by 20mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, RT 15714) in PBS ...
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bioRxiv - Genetics 2022Quote: ... samples were washed 3x in 0.3% v/v PBT and subjected to a second sample fixation in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Genetics 2022Quote: ... adult female intestines were dissected out in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells grown on coverslips were washed in phosphate-buffered saline (PBS) and fixed in freshly prepared PBS supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: KA and saline mice were sedated by vaporizing isoflurane and then intra-cardially perfused with 1X PBS followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA). four days post SE (n = 3 KA and n = 3 saline mice per condition) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary tumors were cut in half and either snap-frozen in dry ice or fixed with 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA). At the endpoint ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Cell Biology 2021Quote: The kidneys containing the grafts were preserved by in vivo intracardiac perfusion in anesthetized mice with heparinized saline buffer (pH 7.4) followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA, USA), and then immersed for 24 hours at 4 °C in the same fixative agent ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion was done with a prewash with 9.25% sucrose in distilled water and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion TwoTM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... at the time of brain microdissection the spinal cord was rapidly dissected and immersed in 4% paraformaldehyde (PFA) in 1× phosphate-buffered saline (PBS) (15710-Electron Microscopy Sciences, Hatfield, PA). For assessment of phospho-cJUN and ATF3 expression animals were perfused with 4% PFA ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Developmental Biology 2022Quote: Wild type (WT) embryos and WT embryos injected with lama1 morpholino were fixed in 2% formaldehyde (Electron Microscopy Science #15710), 2.5% glutaraldehyde (EMS #16220 ...
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bioRxiv - Neuroscience 2019Quote: ... 18 larva of the two different ages were fixed whole in Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde solution diluted in 0.1 M cacodylate buffer at pH 7.4; EMS RT15720; Karnovsky, 1965) for at least 24 hours at 15-20 °C and at a pH of 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Three μL of cell culture were applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Immunology 2022Quote: ... a 2 µl aliquot of EV preparation was placed onto a carbon film grid (Electron Microscopy Sciences, Hatfield, PA, USA) for 1 minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... was adsorbed onto formvar-carbon coated copper grids for 2 min and negatively stained with uranyl acetate replacement stain (UAR-EMS) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Bioengineering 2019Quote: ... agarose RoCs with ROs and RPE were fixed in the chambers with Karnovsky buffer (2.5% glutaraldehyde, 2% paraformaldehyde, 0.1 M sodium cacodylate buffer, pH 7.4) (Electron Microscopy Sciences, Germany) for 12 hours at 4°C ...