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Citations for Electron Microscopy Sciences :
2151 - 2200 of 2878 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Cell coverslips were fixed with 4% paraformaldehyde (prepared from 32% PFA Aqueous So-lution (Cat# 15714, EMS)) with cell morphology and nucleus visualized using Acti-stain 555 phalloidin (1:2000 ...
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bioRxiv - Developmental Biology 2023Quote: Embryos were decapitated and heads were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and the cochlea was perfused with 4% PFA (diluted from 16% PFA, 5710-S, Electron Microscopy Sciences) through the round window ...
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bioRxiv - Neuroscience 2024Quote: ... and an insulin syringe was used to gently flush cold paraformaldehyde (PFA 4%; Electron Microscopy Sciences; 15710) through the cleared oval and round windows after poking a small hole at the cochlear apex ...
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bioRxiv - Neuroscience 2024Quote: Mice were perfused with 20 mL 1XPBS followed by 15mL of 4% PFA (Electron Microscopy Sciences # 15172) diluted in PHEM buffer (0.1 M ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with a mixture of 4% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences, EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with a mixture of 4% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences, EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2024Quote: Neurons were stimulated with 10-Hz burst stimulation and fixed with 4% PFA (Electron Microscopy Sciences #15714) on ice 15 min after the onset of stimulation ...
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bioRxiv - Bioengineering 2024Quote: co-SWIFT vessels and cardiac co-SWIFT tissues were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) for 30 min or 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... cells used for genomic stability analysis were fixed using 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Cat. # 15714) for 15 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Media removed from the well and 300 µl of 4% paraformaldehyde (# 19210, Electron Microscopy Sciences, Hatfield, PA) in PBS was added to the well and cells were fixed for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: For negative staining 3 µl of purified 40S+60S ribosome fractions or alternatively 3 µl of 40S+60S ribosome fractions in the presence of (PR)20 (1:10 molar ratio) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and retained on the grid for 1 min ...
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bioRxiv - Neuroscience 2019Quote: ... and then immersed in 50% ethanol before being dehydrated in ascending concentrations of ethanol (50%, 70%, 90%, 100%) containing 1% uranyl acetate (UA; Electron Microscopy Sciences) with application of microwave irradiation (250 W ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2021Quote: 8 μl of 1 x 1010 pfu/mL of phages was applied to a copper mesh grid coated with formvar and carbon (Electron Microscopy Sciences) for 2 minutes and then gently blotted off with a piece of Whatman filter paper ...
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bioRxiv - Biochemistry 2022Quote: Washed and deglycosylated RBCs were diluted 1:100 and added to carbon coated 400 mesh copper grids (CF400-CU, Electron Microscopy Sciences) that had been glow discharged and pre- coated in poly-d-lysine solution ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were then counterstained with Hoechst 33342 (1:10,000) and mounted using Fluoro-gel in TES Buffer (Electron Microscopy Sciences, 17985-30). The slides were imaged using a Zeiss Observer D1 microscope with a 40x objective.
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bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
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bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
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bioRxiv - Biochemistry 2023Quote: ... In-house graphene grids were prepared from Trivial Transfer Graphene sheets (ACS Material) and QUANTIFOIL R2/1 mesh 300 gold (Electron Microscopy Sciences) by following a protocol introduced by Ahn et. ...
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bioRxiv - Biochemistry 2023Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Developmental Biology 2021Quote: ... gonads or ovaries were fixed in freshly prepared 5% paraformaldehyde (PFA; Electron Microscopy Sciences; Cat# 15714) for 30 min at room temperature ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... and two additional 5 min washes with PBS before mounting with Fluoro-Gel (Electron Microscopy Sciences). A Zeiss Axiovert 200 M wide-field fluorescence microscope was used to collect images ...
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bioRxiv - Cell Biology 2019Quote: ... Fat bodies (FBs) were dissected from larvae in PBS using Dumont #5 forceps (Electron Microscopy Sciences). All FBs were fixed in 4% paraformaldehyde for 30 minutes at room temperature and rinsed briefly in PBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 µL samples were incubated on a copper mesh grid (Formvar/Carbon 300, Electron Microscopy Sciences) for 60 seconds ...
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bioRxiv - Biophysics 2021Quote: ... then incubated in Alexa647 conjugated fAb prior to fixation in 4% PFA and 0.1% gluteraldehide (Electron Microscopy Sciences), as described previously 18 ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were rinsed in PBS and fixed in a 4% paraformaldehyde solution (Electron Microscopy Sciences cat. no. 15710) for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were transferred to an embryological watch-glass and fixed with 4% PFA EM grade (Electron Microscopy Sciences) in PBS at 4°C over-night ...
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bioRxiv - Biophysics 2022Quote: ... 4 µl samples were applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Cultures were fixed at room temperature for 10 min in PBS containing 4 % paraformaldehyde (Electron Microscopy Science 15714S) and 4 % sucrose (Sigma S9378) ...
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bioRxiv - Microbiology 2021Quote: Differentiated epithelial cells grown on a transwell membrane were fixed with 4% paraformaldehyde (Cat#15710, Electron Microscopy Sciences) in PBS for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... slides were immersed three times (4 min) in xylene and mounted on coverslips with Entellam (Electron Microscopy Sciences). Images were taken with a 63x objective in a white field ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells growing on a cover glass (-/+ ARS) were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) in 1 × PBS (phosphate buffered saline ...
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bioRxiv - Biophysics 2022Quote: ... 4 µl of 10 nm BSA-treated gold fiducial beads (Aurion Gold Nanoparticles, Electron Microscopy Sciences, PA, USA) were applied before vitrification ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4% paraformaldehyde (diluted with DPBS from 16% stock, Electron Microscopy Sciences, cat# 15710) and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... pH7.4 (PBS) and fixed for 20 minutes at room temperature in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, RT15710) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... ∼107 tachyzoites growing intracellularly in HFF cells in a T25 flask were incubated for 4 h at 37°C in 0.1% FBS DMEM growth medium containing either 2.5 mM ethyl methanesulphonate (EMS) at final concentration or the appropriate vehicle controls ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...