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Citations for Electron Microscopy Sciences :
2151 - 2200 of 3081 citations for 7 Methyl 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the cells were washed with 100 µl of warm PBS and fixed with 4% methanol-free paraformaldehyde (PFA) (Electron Microscopy Sciences) for 15 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... image positions were recorded and slides were washed in 1xPBS/0.2% Tween 20 to remove the mounting medium and re-fixed in 4% paraformaldehyde (Electron Microscopy Sciences) prior to DNA FISH experiment ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isofluorane (Forane), and then perfused with 4% paraformaldehyde (PFA, Electron Microscopy Services) and 0.005% glutaraldehyde (GA, Electron Microscopy Sciences) in 175 mM sodium phosphate buffer (PB ...
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bioRxiv - Microbiology 2020Quote: ... virus- or mock-infected cells were detached using 0.06% trypsin-EDTA in phosphate-buffered saline (PBS) and fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS for 20 minutes at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... neurons were rinsed with sterile Hank’s Balanced Salt Solution and fixed for 10min with pre-warmed 4% PFA (Electron Microscopy Sciences) in Phosphate Buffered Saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: ... Media was aspirated and cells were washed with PBS at RT once before being incubated with 4% PFA (Electron Microscopy Sciences) made up in PBS with the addition of 4% sucrose at RT for 15 mins ...
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bioRxiv - Cell Biology 2022Quote: ... high parasitemia culture was pelleted by centrifugation (1.5 g, 3min, RT) and resuspended in freshly prepared PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) and 0.0075% gluteraldehyde (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized and transcardially perfused with 0.1M phosphate buffer (PBS) followed by 4% EM-grade paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were dissected and post-fixed overnight in 4% PFA ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were fixed for 15 min at RT with 4% electron microscopy grade paraformaldehyde (PFA; Electron Microscopy Sciences, cat. no. 15710) diluted in PIPES–EGTA– magnesium (PEM ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was removed and the neurons were fixed with 4% electron-microscopy-grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in a cytoskeleton-protective buffer (PEM ...
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bioRxiv - Physiology 2019Quote: Pieces of 0.5 cm2 of whole SC-AT and BM-AT were fixed with a 4% paraformaldehyde solution (PFA, Electron Microscopy Sciences (EMS)) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... a sucrose gradient was created using 2.3 ml of a 20 % sucrose solution containing 15 mM HEPES [pH 7.4] and 4 % paraformaldehyde aqueous solution (EM Grade) (Electron Microscopy Sciences) and 2 ml of a 10% sucrose solution containing 15 mM HEPES [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Monolayers were fixed and plaques visualized with a solution of 0.4% crystal violet by weight in 80% methanol (MilliporeSigma) and 4% PFA (Electron Microscopy Sciences) for 20 min at room-temperature (RT).
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bioRxiv - Biochemistry 2021Quote: Samples (4 μL) were applied to glow-discharged continuous carbon film supported copper grids (CF300-CU-50 grids, Electron Microscopy Sciences). After 1 min incubation ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue for analysis of cytosolic expression of GFP or tdTomato was directly fixed in ice-cold 4% PFA (Electron Microscopy Science) for 2 h at 4° ...
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bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... cerebellums were harvested first and then the same mice were the perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in PBS for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.
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bioRxiv - Bioengineering 2023Quote: Fixation was done by replacing half the volume of media in the culture dish with 4 % paraformaldehyde (Electron Microscopy Sciences; 15714) in phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were rinsed in 1x PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl2) pH 6.8 and fixed in 4% formaldehyde (FA, #15710, EMS, Hatfield, USA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion with phosphate-buffered saline (PBS, Quality Biological, 119-069-491) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) was performed immediately after ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole kidney organoids and mouse kidney explants were fixed for 20 min on ice in 4% PFA (Electron Microscopy Sciences, 15710) in 1xPBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... then fixed for 20 minutes at room temperature (RT) in a fixative comprised of 4% (v/v) paraformaldehyde (Electron Microscopy Sciences), 25% (v/v ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Biophysics 2019Quote: ... and then fixed for 15 minutes at room temperature (RT) with pre-warmed (at 37 °C) 3% paraformaldehyde (PFA, 15710, Electron Microscopy Sciences, Hatfield, PA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Genetics 2023Quote: ... 5-10 μl of 1% PFA solution pH 9.2 containing 0.15% Triton X-100 and 3 mM DTT was placed in each well of a 12-well glass slide (Electron Microscopy Sciences, 63425-05) and one ZP-free oocyte was transferred to each well ...
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bioRxiv - Immunology 2021Quote: ... The grid was washed briefly with 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) and stained for 1 min with the same uranyl formate buffer ...
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bioRxiv - Immunology 2022Quote: ... grids were glow-discharged with 25 mA for 2 min using an EMS 100X (Electron Microscopy Sciences) glow-discharge unit ...
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bioRxiv - Neuroscience 2020Quote: ... transduced neurons on coverslips were fixed in 2% (w/v) paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in 1xPBS with 4% (w/v ...
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bioRxiv - Physiology 2019Quote: SC-AT and BM-AT were fixed with 2,5% glutaraldehyde and 2% PFA (EMS, Hatfield, PA, USA) in Cacodylate buffer (0.1M ...
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bioRxiv - Immunology 2021Quote: ... grids were glow-discharged with 25 mA for 2 minutes using an EMS 100X (Electron Microscopy Sciences) glow-discharge unit ...
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bioRxiv - Genomics 2020Quote: ... Two-step fixation using EM grade 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (EMS) was performed ...
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bioRxiv - Microbiology 2022Quote: ... then washed in 0.1 M cacodylate buffer and post-fixed with 2% osmium tetroxide (Electron Microscopy Sciences) for a 1 h on ice ...
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bioRxiv - Plant Biology 2022Quote: ... The lines ins4/lhw-8 and hyp2/myb12-2 were generated in the dGR background by EMS mutagenesis (see below) ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 and IMR-90 cells were fixed with 2% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat# 15710) for 8-10 min at room temperature ...