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Citations for Electron Microscopy Sciences :
2051 - 2100 of 3233 citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Part of the sample collected as described above was fixed with 2% paraformaldehyde (EMS grade) and 0.5% glutaraldehyde (EMS grade ...
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bioRxiv - Molecular Biology 2023Quote: ... mounted using DAPI (4ʹ,6- diamidino-2-phenylindol) Fluoromount G mounting medium (Electron Microscopy Science) and imaged with an Axio Observer microscope (Zeiss).
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bioRxiv - Biophysics 2023Quote: ... and stained with 2% uranyl acetate and Reynolds lead citrate (Electron Microscopy Sciences, Hartfield, PA). Measurements were performed on the perpendicular cross-sections of 100 hyphae per culture condition using a JEOL JEM-1400 electron microscope (Peabody ...
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bioRxiv - Cell Biology 2023Quote: ... Post-fixation was performed using 2% (w/v) osmium tetroxide (Electron Microscopy Sciences, Hatfield, UAS) for 30 min in chilled cacodylate buffer ...
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bioRxiv - Biophysics 2024Quote: ... samples were thawed and inactivated for 2 hours at room temperature by adding PFA (EMS) to a final concentration of 4 % (v/v) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mg/mL sample was applied to the glow discharged gird (Electron Microscopy Sciences, 25486), followed by removing the extra sample amount using filter paper and quickly plunging into liquid ethane ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Cell Biology 2021Quote: ... fixed for 5 min with 1% glutaraldehyde (Electron Microscopy Sciences).
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bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were dissected in IMADS (Singleton and Woodruff 1994) or 1X PBS and fixed with 4% PFA (Electron Microscopy Sciences) in 1X PBS plus 0.3% Triton-X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... samples were washed once in wash buffer and fixed with a 4% Paraformaldehyde + 0.1% Glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) solution made in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected larvae were fixed for 20 (VNC) or 45 minutes (NMJs) in 4% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... When animals reached the indicated age they were anaesthetized with isoflurane and intracardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences) in PBS was performed ...
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bioRxiv - Immunology 2019Quote: ... The right-side half brains were harvested and fixed in 4% paraformaldehyde (made in PBS; #15710, Electron Microscopy Sciences, USA) at 4◻ for 6 days ...
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bioRxiv - Biochemistry 2020Quote: ... Cell were then fixed for 10 minutes at room temperature in PBSM + 4% paraformaldehyde (Electron Microscopy Sciences 50-980-492). Following fixation ...
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bioRxiv - Neuroscience 2020Quote: ... perfused intracardially with ice-cold phosphate-buffered saline (PBS) and fixed with 4 % paraformaldehyde (PFA, 19210, Electron Microscopy Sciences, USA) in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: Adult mice were anesthetized with isoflurane and transcardial perfusion was performed with 7.5 ml of 1x PBS following by 7.5 ml of ice cold 4% PFA in 1x PBS (EMS – 15710-S). Brains were then dissected out and post-fixed in 4% PFA in 1x PBS for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Post infection coverslips were rinsed in PBS then fixed for 10 minutes in 4% Paraformaldehyde in PBS (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... and lens were gently removed and the resulting posterior eyecup was fixed in freshly prepared ice-cold 4% (w/v) PFA (171010, EMS) in PBS for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... followed by application of 4 μl of the mix onto glow discharged continuous carbon electron microscopy grid (400 mesh, EMS) and was incubated on grid for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... and then 4 μl of the mixture was added to a Quantifoil R1.2/1.3 Au 300 mesh grid (Electron Microscopy Sciences) that had been glow discharged for 60 seconds at 20 mA using a Pelco easiGlow ...
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bioRxiv - Bioengineering 2022Quote: ... the media is removed from the wells and the cells are fixated via a 4% paraformaldehyde (PFA, Electron Microscopy Sciences) solution incubation for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Fixation buffer consisting of 4% paraformaldehyde (Alfa Aeser, USA, CAT#:43368) and 0.1% glutaraldehyde (Electron Microscopy Sciences, USA, CAT#: 16100) was used to fix cells on the coated surfaces.
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bioRxiv - Neuroscience 2022Quote: ... the recipient mice eyes and cultured retinal organoids were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) in PBS and dehydrated in a sucrose gradient (10% ...
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bioRxiv - Neuroscience 2022Quote: ... animals were transcardially perfused with ice cold 1x PBS followed by ice cold 4% PFA (Electron Microscopy Sciences, 100504-858). Spinal cords were dissected and post-fixed in 4% PFA for 2 hours at 4°C ...
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bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with 1X PBS and fixed in 4% paraformaldehyde (PFA) solution (diluted from 16% PFA, Electron Microscopy Sciences) in PBS for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with ketamine/xylazine before transventricular cardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA; Cat #19210) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2019Quote: ... 10cm long) were gently swiss rolled from the distal end and fixed in 4% paraformaldehyde (Electron Microscopy Science, Hatfield USA). Formalin-fixed tissues were then processed for paraffin embedding ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μL solution in each reaction condition was immediately transferred to a glow-discharged continuous carbon grid (Electron Microscopy Sciences) for 15 seconds before blotting and staining ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with 0.9% NaCl followed by a mixture of 4% PFA and 0.1% glutaraldehyde (Electron Microscopy Sciences) in 0.1M phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Neuroscience 2023Quote: E16.5 and E18.5 embryos were sacrificed by head sectioning and brains were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in Phosphate buffered saline (PBS ...