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Citations for Electron Microscopy Sciences :
2051 - 2100 of 2340 citations for 6 Bromo 2 methylthiazolo 5 4 b pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with pentobarbital and perfused with 2% paraformaldehyde (PFA, Paraformaldehyde Granular, Electron Microscopy Sciences, Hatfield, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... (2) fixed with 1.5% paraformaldehyde and 1.5% glutaraldehyde in 0.1M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA, USA), pH 7.3 for 1 h at room temperature followed by 3 washes with 0.1M sodium cacodylate ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Ovaries used for MIMS were cut in half and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in LR white (EMS) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Plant Biology 2019Quote: ... using fine forceps and a 27-gauge needle as a scalpel, developing seeds were dissected from siliques and immediately immerged in fix solution (1×PBS, 4% formaldehyde (Electron Microscopy Sciences, 15713), and 0.4% dimethyl sulfoxide ...
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bioRxiv - Cell Biology 2020Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were washed with DPBS and then fixed with 4% paraformaldehyde (diluted from a 16% solution; Electron Microscopy Sciences cat. no. 15710) for 15 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence analysis of PGCLC bodies or rOvaries was performed on cryosections prepared as follows: aggregates were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science, 15713) in PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to a scintillation vial containing a 1:1 (v/v) mixture of heptane and 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) in PBS on a shaker for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with 20 mL ice cold 1X DPBS followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA). Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 3844-45-9) was purchased from Wako Pure Chemical Industry (Japan). Paraformaldehyde (CAS No. 30525-89-4) was purchased from Electron Microscopy Sciences (USA). NaCl (CAS No ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were taken on either a Leica DMi8 (Hatch Lab microscope) or Leica spinning disk microscope with a 40x confocal objective after being fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 5 min at room temperature with coverslips mounted on glass slides with VectaShield anti-fade medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of particles in suspension were mounted on a copper grid of Formvar 300 mesh/carbon (Electron Microscopy Science, PA). Samples were dried at room temperature for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at the Pole Facultaire de Microscopie Electronique at the University of Geneva as described 5 except uranyl acetate staining was replaced with Reynold’s (lead citrate, Electron Microscopy Sciences), and 20 nm sections were used ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min and the coverslips were mounted onto glass slides with CitiFluor AF1 antifading (Electron Microscopy Sciences, Emsdiasum, Hatfield Penn). Images were acquired with an inverted 3I spinning disk confocal microscope (Zeiss Observer Z.1 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Immunology 2020Quote: ... ~ 5 μL of the sonicated samples were drop cast onto 300-mesh formvar carbon-coated copper grids (Electron Microscopy Sciences, UK) and negatively stained with UranyLess (Electron Microscopy Sciences) ...