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Citations for Electron Microscopy Sciences :
1951 - 2000 of 2708 citations for 6 Fluoro 4 hydroxy 1 7 naphthyridine 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibody incubation was followed by 4 x 15-minute washes in 1X PBS and slide mounting using Fluor-Gel mounting medium (Electron Microscopy Sciences). Tissues were mounted epithelium-side up.
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on 35-mm Glass Based Dishes which have a cover glass with a grid pattern (IWAKI) and fixed after laser-microirradiation with 4% PFA (Electron Microscopy Sciences) containing 0.1% Triton X-100 (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2023Quote: For wholemount immunofluorescent labeling heads were bisected and brains removed prior to immersion fixation of the intact inner ear and skull in 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences) prepared in 67mM Sorensons phosphate buffer (pH 7.4) ...
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bioRxiv - Developmental Biology 2023Quote: ... the sample holder was removed from the microscope in order to perform sample fixation using 4 % PFA (Catalog no. 15714, Electron Microscopy Sciences) and 0.08 % glutaraldehyde (Catalog no ...
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Developmental Biology 2023Quote: ... Adherent cells (at ESC stage for the CRISPRa validations and PP stage for the CRISPRi validations) were rinsed once with PBS with Ca2+ and Mg2+ (PBS++) and fixed with 4% Paraformaldehyde (Electron Microscopy Sciences 32% Paraformaldehyde ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Microbiology 2023Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice in warm 1X HBSS and fixed for 15min in 4% paraformaldehyde (PFA) made from fresh 16% PFA (#15710, Electron Microscopy Sciences) diluted with 1X HBSS ...
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bioRxiv - Bioengineering 2023Quote: Rats implanted with MEV probes were anaesthetized with 3% isoflurane or 300 mg kg−1 of ketamine (Patterson Veterinary Supply) and transcardially perfused with ice-cold 30 ml saline and 30 ml 4% paraformaldehyde (PFA, Electron Microscopy Sciences) at specified times post-injection ...
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bioRxiv - Neuroscience 2023Quote: ... the monkeys were deeply anesthetized (ketamine, 10 mg/kg and sodium pentobarbital, 100 mg/kg) and transcardially perfused with 4% formaldehyde (EMS, Hatfield, PA) in Phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with PBS (containing CHX for cells treated with it) and fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS for 10 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and xylazine (60 mg/kg) then transcardially perfused with 10mL of PBS and 40mL of freshly hydrolyzed 4% paraformaldehyde (19210, Electron Microscopy Sciences). Tissues were gently dissected and placed in 4% paraformaldehyde for post fixation ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Genetics 2023Quote: Testis and epididymides samples from 8-16-week-old mice were fixed for 24 h in PBS/4% PFA (Electron Microscopy Sciences), dehydrated in a graded ethanol series ...
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bioRxiv - Cell Biology 2023Quote: Cells seeded in 24-well plates on poly-D-Lysine-coated glass coverslips were washed once in PBS and fixed with 4 % methanol-free PFA (Electron Microscopy Science) for 15 minutes at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: Mice with implanted mesh probes were anaesthetized and transcardially perfused with ice-cold 40 ml PBS and 40 ml 4% formaldehyde (Electron Microscopy Sciences) at specified times post-implantation ...
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bioRxiv - Neuroscience 2023Quote: ... and xylasine (10 mg/kg) and then intracardially perfused with ice-cold PBS and ice-cold 4% paraformaldehyde dissolved in PBS (PFA) (Electron Microscopy Sciences). Brains ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Mice of desired age and genotype were sacrificed by cervical dislocation and their eyes were immediately collected and immersed in 4% PFA in PBS (n°15714S, Electron Microscopy Sciences). They were incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: mESCs derived MNs were plated on pre-coated 12 mm diameter coverslips and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and transcardial perfusion was performed with 10 mL of 1x PBS followed by 10 mL of ice cold 4% PFA (EMS, #15710-S) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with pentobarbital (Fatal-Plus, Vortech Pharmaceuticals) and intracardially perfused with ∼50 ml of 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... 200-071, Virbac) and transcardially perfused with 30mL of PBS (BP399, Fisher bioreagents) followed by 50mL of 4% PFA (18210, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Biochemistry 2024Quote: Cryo-grids were made by evaporating a 20 nm gold layer with ∼4 µm holes on the rhodium side of 400-mesh copper-rhodium grids (Electron Microscopy Sciences) in a Leica EM ACE200 Vacuum Coater ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature (25°C) with 4% PFA (Electron Microscopy Sciences) diluted in PBS and stored at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Shoot apices at different developmental stages were dissected under a stereomicroscope and fixed with 4% (v/v) paraformaldehyde (PFA; Electron Microscopy Sciences). The fixed samples were washed twice for 1 min in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the cornea and the lens were removed from the eyes and the eyecups were further fixed by immersion in 4 % paraformaldehyde (PFA, EMS Cat#15710) for 30 minutes (min ...
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bioRxiv - Biophysics 2020Quote: ... 1% Tannic acid (EMS) and 1 % Uranyl acetate (EMS) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% osmium tetroxide (EMS) in 0.1M cacodylate buffer was used as a secondary fixative with incubation for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% GA (EMS) in 0.1M Phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 1% uranyl acetate (EMS), and 0.66% aspartic acid buffered lead (II ...
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Cell Biology 2022Quote: ... followed by staining in Sato’s lead (a solution of 1% lead acetate, 1% lead nitrate,and 1% lead citrate; all from Electron Microscopy Sciences) for 2 minutes ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... followed by staining in Sato’s lead (a solution of 1% lead acetate, 1% lead nitrate, and 1% lead citrate; all from Electron Microscopy Sciences) for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... infiltrated in 1:1 volumes of 100% ethanol and 100% LR White (EMS) during 4 h ...
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bioRxiv - Cell Biology 2020Quote: ... and multiple open ended embedding capsules with a 1 × 1 cm face (EMS) were placed on the coverslips covering the areas of interest ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 1% uranyl acetate in water (Electron Microscopy Sciences 22400-1) was added to the grid ...
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bioRxiv - Cell Biology 2020Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were washed with DPBS and then fixed with 4% paraformaldehyde (diluted from a 16% solution; Electron Microscopy Sciences cat. no. 15710) for 15 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence analysis of PGCLC bodies or rOvaries was performed on cryosections prepared as follows: aggregates were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science, 15713) in PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...