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Citations for Electron Microscopy Sciences :
151 - 200 of 209 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... TolAQR (abs280=5) or TonB-ExbBD (abs280=1.4) were applied to a Quantifoil R1.2/1.3 300 mesh grid (Electron Microscopy Sciences, Protochips, Inc.) that had been glow discharged for 45 s at 15 mA (Pelco easiGlow ...
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bioRxiv - Neuroscience 2024Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... main inflorescences (not side branches) were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed three times (650xg, 5 min, RT) with Perm Buffer before fixation in 1.6% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS overnight with 125nM Iridium (Fluidigm ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µl of protein complex was deposited on a holey copper grid (QUANTIFOIL R1.2/1.3, 200 mesh, EMS cat# Q250-CR1.3) or a carbon film copper grid (QUANTIFOIL R 1.2/1.3 ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were diluted 10-fold in liposome buffer and 5 μL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). After 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Bioengineering 2021Quote: ... ∼5 μL of each sEV-particle complex was left to adhere onto formvar carbon coated copper Grid 200 mesh (Electron Microscopy Sciences) for 5 mins followed by 5 mins of negative staining with 2% aqueous uranyl acetate ...
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bioRxiv - Neuroscience 2020Quote: ... Each coverslip was mounted on a stub with carbon tape and samples were sputter coated with iridium at 10μA to a thickness of approximately 5 nm (EMS model 150T S). The coverslips were then examined on a Hitachi S4300 SEM (Hitachi High Technologies America Inc. ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... We applied 5 μL RBC-EV sample to a 200 mesh copper grid coated with formvar carbon film (FCF200-Cu, Electron Microscopy Sciences) for 30 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Microbiology 2023Quote: ... and 150 mM NaCl buffer and 5 µL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). Grids were blotted after 1 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then washed three times in PBS for 5 minutes each prior to cover slipping using Fluorogel mounting medium (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Immunology 2024Quote: ... and 5% glycerol and applied to glow-discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 2% uranyl formate (UF) ...
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bioRxiv - Cell Biology 2024Quote: ... cells grown on 13-mm round poly-L-lysine-coated coverslips were fixed by adding to the culture medium an equal volume of 4% PFA (Electron Microcopy Sciences) and 5% glutaraldehyde (GTA) (Electron Microscopy Sciences) in 0.2 M cacodylate pH 7.4 for 15 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
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bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed three times for 5 min in PBS 1X and mounted using Fluoromont G fluorescence mounting medium (EMS, No. 17984-25). For all immunofluorescence analysis ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Cancer Biology 2024Quote: ... the samples were rinsed 3 times for 5 min each in PB buffer and postfixed with a fresh mixture of 1% osmium tetroxide (EMS, Hatfield, PA, US) and 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Immunology 2021Quote: 5 µl of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater, Electron Microscopy Science, Hatfield, PA). Using the Zeiss SmartSEM software ...
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bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were stained en bloc with 0.25% uranyl acetate then dehydrated in graded series of ethanol on ice followed by one wash with 100% ethanol and two washes with propylene oxide (5 min each) and embedded with EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Sections were cut at 60 nm on a Leica EM UC6 ultramicrotome ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The vaccine powder was reconstituted and diluted 5 times with water and then transferred to a glow discharge-treated carbon coated copper grid from Electron Microscopy Sciences (Hatfield, PA). The sample was stained with a 2% uranyl acetate solution for 2 min and then air dried ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Biophysics 2024Quote: ... cells were imaged under physiological conditions (5% CO2 and 37°C) via a stage top incubator (Tokai Hit) with type 37 immersion oil (Electron Microscopy Sciences, #16914-01). Fixed cells were imaged at room temperature (22°C ...
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bioRxiv - Biochemistry 2024Quote: Protein samples were diluted to 0.1 to 0.15 mg/mL and applied to freshly glow discharged (60 s, 5 mA, PELCO easiGlow) 200 mesh gold formvar/carbon square mesh grids (EMS, Cat # FCF200-AU-EC). After 60 s ...