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Citations for Electron Microscopy Sciences :
151 - 200 of 224 citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: Purified rAAV8 samples (human-produced empty and full, baculovirus-Sf9 produced empty and full) were applied to Lacey carbon Quantifoil grids (EMS Cat# Q225CR-06) and flash frozen ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of concentrated pseudovirus sample was applied to a carbon-coated grid (2/2-4C C-flats; Electron Microscopy Sciences), blotted with filter paper ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Hippocampi from 6-month-old PS19 mouse brains with and without 3-month glutamine administration were cut into small specimens (one dimension < 1 mm) and fixed in Trump’s fixative (Electron Microscopy Sciences) for 2 hr at RT ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 5min in 200mM KCl to stop heart beating and fixed for 2-3 hours in 4% PFA (Electron Microscopy Science, #15710S) at 4°C.
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 culture was pelleted by centrifugation (1500g, 3 min, RT) and resuspended in freshly prepared PBS containing 4% EM-grade para-formaldehyde (PFA, Electron Microscopy Sciences) and 0.1% EM-grade GA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Biochemistry 2023Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Microbiology 2023Quote: ... 3 μl of this mixture was blotted on a glow-discharged Quantifoil R 2/1 Copper 300 mesh grid (Electron Microscopy Sciences) and plunge-frozen in liquid ethane using a Vitrobot plunge freezer (ThermoFisher Scientific ...