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Citations for Electron Microscopy Sciences :
151 - 200 of 219 citations for Nipah Virus Glycoprotein G Recombinant Protein Human Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... high parasitemia culture was pelleted by centrifugation (1.5 g, 3min, RT) and resuspended in freshly prepared PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) and 0.0075% gluteraldehyde (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Neuroscience 2021Quote: ... PCW17 and PCW21 human cortical tissue was fixed overnight at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Samples were then washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes at RT and washed twice with ice-cold PBS before mounting onto glass slides with 5 µl Fluoromount-G mounting medium (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2020Quote: ... Microscopy slides (76 × 26 mm, Glaswarenfabrik Karl Hecht GmbH & Co. KG) were coverslipped using Fluoromount-G aqueous mounting medium (Electron Microscopy Sciences). Confocal imaging was performed using an Olympus FV1200MPE confocal microscope (Olympus Europa SE & Co ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were finally washed in PBS-Tx and rinsed in water before mounting onto slides with Fluoromount G with DAPI (Electron Microscopy Sciences) for microscopic analysis.
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent secondary antibodies were incubated in 1% BSA-PBS solution for 1 h at room temperature before being mounted in Fluoromount G (Electron Microscopy Sciences). Acquisitions were performed on an LSM 800 confocal laser-scanning microscope (Zeiss) ...
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bioRxiv - Microbiology 2020Quote: ... Each protein was deposited on a CF-400-CU grid (Electron Microscopy Sciences) that had been plasma cleaned for 30 seconds in a Solarus 950 plasma cleaner (Gatan ...
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bioRxiv - Synthetic Biology 2020Quote: Purified protein samples applied to CF300-Cu carbon coated grids (Electron Microscopy Sciences) were prepared for negative stain transmission electron microscopy ...
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bioRxiv - Cell Biology 2019Quote: Single β-cells from dispersed human islets were cultured on 25mm round coverslips from Electron Microscopy Sciences (cat# 72225-01) overnight in the human islet media describe above ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... centrifuged at 3,000 x g for 5 min and clarified samples incubated for 2 h on carbon-coated Formvar nickel grids (300 mesh, Electron Microscopy Science, Pennsylvania) coated with anti-GFLV polyclonal antibodies (Bioreba AG ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times with PBS (pH 7.4) and mounted in slides cover with gelatin using Fluoromount-G (Electron Microscopy Sciences, cat n° 17984-25).
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bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 hour the leaf discs were washed three times with sterile water and then mounted in Fluoromount-G ™ (Electron microscopy Sciences, Hatfield, PA, USA). Leaf discs were imaged with a Zeiss LSM800 Airyscan laser scanning confocal microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Molecular Biology 2020Quote: Purified rAAV8 samples (human-produced empty and full, baculovirus-Sf9 produced empty and full) were applied to Lacey carbon Quantifoil grids (EMS Cat# Q225CR-06) and flash frozen ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein was adsorbed on freshly glow-discharged carbon-film grid (CF200-CU, Electron Microscopy Sciences), washed with dilution buffer and stained with 0.75% w/v uranyl formate ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cover slips were mounted on a glass slide with 20 μL of Fluoromount-G containing DAPI (Electron Microscopy Sciences, Hatfield, PA, USA; Cat:17984-24). Confocal microscopy images were obtained using an LSM 700 Zeiss confocal microscope (Carl Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Neuroscience 2021Quote: ... the beads with enriched proteins were washed three times with 80% ethanol (Electron Microscopy Sciences Cat. #15055) followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were deposited on formvar/carbon-coated 200-mesh copper grids (FCF200-Cu, Electron Microscopy Sciences) for 1 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... A 3.5 μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences) that had been glow discharged for 10 seconds using an easiGlow Glow Discharge Cleaning System (PELCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2.2µL drop of protein was deposited on a Quantifoil R1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...