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Citations for Electron Microscopy Sciences :
151 - 200 of 240 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... was subsequently applied on a glow-discharged C-flat CF-1.2/1.3 400 mesh holey carbon grids (Electron Microscopy Sciences), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated adult eyes were fixed for 1 hour at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1X PBS ...
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bioRxiv - Microbiology 2024Quote: ... Carbon-coated copper grids (200 mesh) and C-flat cryo-EM grids were purchased from Electron Microscopy Sciences (Hatfield, PA). Lacey carbon cryo grids and 2% (w/w ...
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bioRxiv - Physiology 2024Quote: ... slides were heated for 30 minutes at 65°C followed by submersion in Histoclear II (Electron Microscopy Sciences 64111-04) twice for five minutes ...
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bioRxiv - Neuroscience 2024Quote: ... animals were transcardially perfused with 20 mL of ∼37°C PBS 0.1M followed by 20 mL of ice-cold 4% paraformaldehyde (EMS, 15714S) in PBS 0.1M ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were fixed for 2 hours at room temperature or overnight at 4° C in 4% paraformaldehyde (EMS catalog # 15710) and 4% sucrose (MP Biomedicals catalog # 802536 ...
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bioRxiv - Cell Biology 2020Quote: Cells were cultured on cell tight C-2 cell disks (Sumitomo Bakelite) and fixed for 2 h in 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples were immersion-fixed for at least one overnight at 4°C in 2.5% glutaraldehyde + 4% paraformaldehyde (Electron Microscopy Science) in a 1mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Cell Biology 2021Quote: ... Post-fixation was performed for 1 hour at 4 °C using 1 % osmium tetroxide and 0.8 % potassium ferracyanide (Electron Microscopy Sciences) in 0.1 mol/l cacodylate buffer ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of sample was loaded on freshly glow discharged 1.2-1.3 300 mesh C-flat™ grids without further support (Electron Microscopy Sciences). The Vitrobot chamber was set to 4 °C and 95% humidity and grids were prepared with a range of blotting time and force ...
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bioRxiv - Cancer Biology 2022Quote: ... Target retrieval was performed in citrate buffer at pH 6.0 for 15 minutes at 110°C under pressure and sections were transferred to Sequenza slide staining racks (Electron Microscopy Sciences). Sections were treated with Bloxall (Vector labs ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of concentrated pseudovirus sample was applied to a carbon-coated grid (2/2-4C C-flats; Electron Microscopy Sciences), blotted with filter paper ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Developmental Biology 2023Quote: ... as well as P14 Hmgb1flx/flx and Hmgb1ΔAstro animals was extracted and fixed overnight at 4°C in 4% RNase-free PFA (Electron Microscopy Sciences) in sterile PBS ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4 buffer) was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... From this stock a 3.5 µl drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Microbiology 2023Quote: ... and the transwell insert was washed with Dulbecco’s phosphate-buffered saline (DPBS) and fixed overnight at 4°C with cold 4% paraformaldehyde (PFA; Electron Microscopy Sciences) diluted in DPBS ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Microbiology 2022Quote: Bacteria were grown in Luria Broth liquid culture overnight at 37°C and immobilized on a Formvar grid (Electron Microscopy Sciences) for 1 min 30 sec and the samples were negatively stained with 0.5% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... The specimens were finally polymerised at 70°C for 72 h in flat embedding silicon molds (#70905-01, EMS, Hatfield, USA).
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Molecular Biology 2024Quote: ... a 3.5 μL drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) with a 4 nm carbon coat ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The testes were extracted and fixed for two days at 4 °C in 4 % PFA (Electron Microscopy Sciences, Hatfield, PA ,USA). Samples were then washed with PBS for at least two days ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were submerged in 1.5% uranyl acetate in absolute methanol at -90 °C for 2 days and infiltrated with HM20 Lowicryl resin (Electron Microscopy Sciences) over 2 days at -45 °C ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The 40 μm cryosections were cut at -18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were placed on a Durcupan block with a flat surface and covered with ACLAR® 33 C Film (Electron Microscopy Science). Samples were let polymerize for 72 h at 60 °C.
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µl of each sample was applied to the plasma-cleaned C-flat grids (Electron Microscopy Sciences, CF-1.2/1.3-4Au-50), pre-blotted and blotted with filter paper (Whatman 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...