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Citations for Electron Microscopy Sciences :
151 - 200 of 287 citations for 6 nitrobenzo d isoxazol 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-gold was modeled at the same size as secondary gold (6-nm anti-mouse and 10-nm anti-rabbit, Electron Microscopy Sciences) and colored similarly to the closest membrane as indicated in the Figure Legends ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... pure resin at 30°C for 2 × 12 h and pure resin with 3% accelerator (BDMA, EMS) at 30°C for 12 h ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
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bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
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bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... plated in glass bottom dishes or hESC-derived striatal neurons on coverslips were washed in D-PBS and fixed for 10 min in 4% PFA (Electron Microscopy Sciences). The cells were washed 3 times with D-PBS and incubated with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: Cells seeded in 24-well plates on poly-D-Lysine-coated glass coverslips were washed once in PBS and fixed with 4 % methanol-free PFA (Electron Microscopy Science) for 15 minutes at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
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bioRxiv - Cell Biology 2023Quote: Wandering third-instar larvae were dissected in ice cold Ca2+-free saline and fixed for 6 minutes in Bouin’s Fixative (Electron Microscopy Sciences, Cat# 15990). Dissections were washed several times with 1X PBS and then permeabilized with 1X PBS containing 0.1% Triton-X (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times with PBS and then incubated with a 4% PFA solution (Electron microscopy sciences) for 30 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Biophysics 2022Quote: 3 µL of sample was pipetted onto Quantifoil R1.2/R1.3 holey carbon grids (Cu 400, Electron Microscopy Sciences) which had been glow discharged for 1 min using a PELCO easiGlow glow discharge cleaning system (Ted Pella) ...
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bioRxiv - Biophysics 2023Quote: ... 3 μL of the sample was applied to glow-discharged (45 s) Quantifoil (2/2; Electron Microscopy Sciences) grids ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3 times in 1X PHEM buffer and post-fixed in 2% OsO4 (Electron Microscopy Sciences) +1,5% potassium ferrocyanide in water 1h in the dark ...
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bioRxiv - Genomics 2024Quote: ... samples were washed twice with PBS-T prior to post-fixation in 3% formaldehyde (Electron Microscopy Sciences 15714) and 0.1% glutaraldehyde (Electron Microscopy Sciences 16120 ...
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bioRxiv - Cell Biology 2024Quote: ... the sections were then transferred to a 3:1 mixture of propylene oxide: Araldite resin (EMS, Cat#13940) for overnight incubation under gentle rolling at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times with 1X PBS and mounted on glass slides using Hydromount (Electron Microscopy Sciences).
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bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Cell Biology 2019Quote: ... Cells were allowed to grow for 6 h and then compressed for 18 h before being fixed with 4% paraformaldehyde (# 30525-89-4, Electron Microscopy Sciences, Hatfield, PA). The non-invasive cells on the upper chamber were removed with cotton swabs ...
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bioRxiv - Neuroscience 2022Quote: ... The mammary gland was excised and fixed overnight from either lactating wild-type (C57BL/6) or oxytocin knockout mice in 4% PFA (Electron Microscopy Sciences, Hatfield, PA). Tissue was incubated at 37 °C in CUBIC Reagent 1 A (10 wt% Triton ...
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bioRxiv - Genomics 2023Quote: ... cells in the 6 well glass bottom plates were fixed by aspirating the medium and replacing it with 4% paraformaldehyde (Electron Microscopy Sciences, 15710-S) in a 1x RNase free PBS solution made from 10x RNase Free PBS (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... The labella of flies (6-8 days old) were dissected and fixed in a solution containing 4% paraformaldehyde (Electron Microscopy Sciences, Cat No 15710) and 0.2% Triton X-100 for 15 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: ... The brain was removed from the skull and a ∼6 mm thick section collected from the optic chiasm to the mamillary bodies using a rat coronal brain matrix (Electron Microscopy Sciences, 69083-C). The hypothalamus was isolated by removing brain matter above the top of the third ventricle and lateral to the optic tract ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Cell Biology 2019Quote: ... a 3 μL drop of the mixture was placed on a glow discharge continuous carbon grid (Electron Microscopy Science) and stained with 2% uranyl acetate ...
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bioRxiv - Developmental Biology 2020Quote: Organoid cultures were washed 3 times with PBS and fixed for 30 min in 4% paraformaldehyde (Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2021Quote: Washed platelets in a concentration of 3 x 105 platelets/μl were fixed with 2.5% glutaraldehyde (Electron Microscopy Science) in cacodylate buffer (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of LcTwinkle-DNA sample was deposited onto a freshly glow-discharged CF400-CU carbon grid (EMS Inc.). After absorbing the extra protein solution with filter paper ...
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bioRxiv - Cell Biology 2022Quote: ... as model cargos and placed in the incubator for 15 min or 3 h incubation followed by washing with PBS and fixation with 2.5% glutaraldehyde (EMS) in 0.1 M phosphate or cacodylate buffer ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... 3 uL of sample was blotted on glow-discharged UltraAuFoil EM-grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences), with 3 s of wait time and 7 sec of blot time ...
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bioRxiv - Microbiology 2022Quote: ... 3 μL of this suspension was deposited on glow-discharged 300 mesh formvar/carbon copper grids (Electron Microscopy Sciences), washed once with water and stained with 0.5% w/v phosphotungstic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The cultures were grown for 3 hours with vigorous shaking every 30 minutes (to keep the EMS in solution). The cultures were passed out of the chamber and centrifuged at 3,000 x g for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Tissues were fixed between 15 minutes to 3 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Post-fixation samples were dehydrated with ethanol ...