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Citations for Electron Microscopy Sciences :
1901 - 1950 of 2201 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue for analysis of cytosolic expression of GFP or tdTomato was directly fixed in ice-cold 4% PFA (Electron Microscopy Science) for 2 h at 4° ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... cerebellums were harvested first and then the same mice were the perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in PBS for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Bioengineering 2023Quote: Fixation was done by replacing half the volume of media in the culture dish with 4 % paraformaldehyde (Electron Microscopy Sciences; 15714) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... or 30 min and fixed by adding an equal volume of 4 % pre-warmed paraformaldehyde (Electron Microscopy Sciences, final concentration 2 %), followed by 20 min incubation at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were rinsed in 1x PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl2) pH 6.8 and fixed in 4% formaldehyde (FA, #15710, EMS, Hatfield, USA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2023Quote: ... The block edges were covered with additional strips of carbon tape and 2-4 strips of copper tape (EMS; cat#77802) and coated with a layer of colloidal silver liquid (EMS ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were washed with cold PBS supplemented with 0.1 mM CaCl2 and 2 mM MgCl2 (PBSCM) and fixed in 4% paraformaldehyde (Electron Microscopy Sciences)/4% sucrose for 8 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion with phosphate-buffered saline (PBS, Quality Biological, 119-069-491) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) was performed immediately after ...
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bioRxiv - Developmental Biology 2023Quote: Wing imaginal discs dissected from late third instar larvae in 1×PBS (pH 7.4) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 40[minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... MFPs were fixed in cold 2% v/v glutaraldehyde with 4% paraformaldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) for 4 hr followed by one wash in the same buffer ...
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bioRxiv - Microbiology 2023Quote: Mice lungs were perfused with 8 mL PBS and inflated with 0.7 mL of OCT compound (Tissue-Tek) mixed 1:1 v/v with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole kidney organoids and mouse kidney explants were fixed for 20 min on ice in 4% PFA (Electron Microscopy Sciences, 15710) in 1xPBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... then fixed for 20 minutes at room temperature (RT) in a fixative comprised of 4% (v/v) paraformaldehyde (Electron Microscopy Sciences), 25% (v/v ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Biophysics 2019Quote: ... and then fixed for 15 minutes at room temperature (RT) with pre-warmed (at 37 °C) 3% paraformaldehyde (PFA, 15710, Electron Microscopy Sciences, Hatfield, PA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Genetics 2023Quote: ... 5-10 μl of 1% PFA solution pH 9.2 containing 0.15% Triton X-100 and 3 mM DTT was placed in each well of a 12-well glass slide (Electron Microscopy Sciences, 63425-05) and one ZP-free oocyte was transferred to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown on 35 mm Glass Based Dishes or 24-well EZVIEW Glass Bottom Culture Plates LB (IWAKI) and fixed with 4% PFA (Electron Microscopy Sciences) for 15 min ...
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bioRxiv - Biophysics 2019Quote: MCN were fixed from 15th to 19th day in vitro (DIV) with 4 % EM grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in PEM buffer (80 mM PIPES ...
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Tape containing sections was attached to a 4-inch silicon wafer in rows using double sided carbon tape (EMS, Cat# 77817-50) and sections were post-stained with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were fixed using 4% paraformaldehyde (Serva, Heidelberg, Germany) and 2% glutaraldehyde (Serva) in PBS followed by staining with 0.5% osmium tetroxide (EMS, Hatfield, PA, USA). After thorough rinsing in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen (invadopodia formation assay) or gelatin-coated coverslips (degradation assay) and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue-HDMI-array sandwich was moved to room temperature, and the tissues were fixed in 4% formaldehyde (100 μl, diluted from the EM-grade 16% paraformaldehyde (#15170, Electron Microscopy Sciences)) for 10 min.
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... then fixed for 45 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at room temperature for 45 min ...
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bioRxiv - Physiology 2022Quote: ... Adult fish were cardiacally perfused with a solution of 4% paraformaldehyde (PFA) diluted in phosphate buffered saline (PBS) solution (Electron Microscopy Sciences) to remove autofluorescence from blood cells40 ...
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bioRxiv - Cell Biology 2022Quote: ... the unroofed cells were placed in fresh 4% PFA (for immunofluorescence) or in 2% glutaraldehyde (for TEM, EM grade, Electron Microscopy Science) for fixation at 4C.
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bioRxiv - Neuroscience 2021Quote: At the end of experiments mice were perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science, USA) in PBS ...