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Citations for Electron Microscopy Sciences :
1851 - 1900 of 2685 citations for 8 BROMO 2 3 4 5 TETRAHYDRO 1H PYRIDO 4 3 B INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence analysis of PGCLC bodies or rOvaries was performed on cryosections prepared as follows: aggregates were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science, 15713) in PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to a scintillation vial containing a 1:1 (v/v) mixture of heptane and 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) in PBS on a shaker for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with 20 mL ice cold 1X DPBS followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA). Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... a section from the thoracic part of the spines were embedded in an 8×8 mm tissue embedding mold (Electron microscopy sciences, 70180) by freezing in Tissue-Tek OCT (Sakura ...
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bioRxiv - Cancer Biology 2021Quote: ... 002% NaN3, and 2 mM EDTA in PBS 1X) and centrifuged (500 × g, 5 min) before adding paraformaldehyde (PFA, EMS) at a final concentration of 16% for 10 min at room temperature in FCB ...
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bioRxiv - Immunology 2020Quote: ... slides were washed twice with MIBI wash buffer and fixed for 5 min in 2% glutaraldehyde solution (Electron Microscopy Sciences) in low-barium PBS ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mounting media (~150 μL/slide) was prepared the day of use by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Developmental Biology 2019Quote: Mice were sacrificed according to ethical guidelines and inner ears were dissected out in cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were subjected to intracardiac perfusion with 5mL of PBS containing heparin followed by 20mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, RT 15714) in PBS ...
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bioRxiv - Genetics 2022Quote: ... samples were washed 3x in 0.3% v/v PBT and subjected to a second sample fixation in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Genetics 2022Quote: ... adult female intestines were dissected out in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells grown on coverslips were washed in phosphate-buffered saline (PBS) and fixed in freshly prepared PBS supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: KA and saline mice were sedated by vaporizing isoflurane and then intra-cardially perfused with 1X PBS followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA). four days post SE (n = 3 KA and n = 3 saline mice per condition) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary tumors were cut in half and either snap-frozen in dry ice or fixed with 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA). At the endpoint ...