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Citations for Electron Microscopy Sciences :
1851 - 1900 of 1945 citations for 4 Nitrophenyl beta D glucuronic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are washed with culture medium and fixed for 2 minutes with room temperature fixative (4% EM grade paraformaldehyde (Electron Microscopy Sciences #19202) + 0.1% EM grade glutaraldehyde (Ted Pella ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized by intraperitoneal injection of 100 mg/kg ketamine /20 mg/kg xylazine (Anased) mix and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences 50980487). Brains were dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: About ∼50 million H1-GATA6+/+ cells at day 2 or day 4 of cardiac differentiation were fixed with 1% formaldehyde (Electron Microscopy Sciences 15710) for 8 min at RT with gentle agitation ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were initially washed with 1X Assay buffer (GOLGI-ID kit) and then fixed with 4% of 16% paraformaldehyde solution (Electron Microscopy Sciences, 15710) at room temperature for 10 minutes before staining the cells for detection of Golgi bodies using the manufacture’s manual for “Staining Adherent Fixed Cells” ...
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bioRxiv - Neuroscience 2024Quote: ... the 33 mice were deeply anesthetized with isoflurane and transcardially perfused with 10mL of ice-cold PBS followed by 10mL of 4% paraformaldehyde (16% PFA diluted in PBS; Electron Microscopy Sciences 15710). The olfactory bulbs (OBs ...
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bioRxiv - Neuroscience 2024Quote: At 10-15 days in vitro (DIV) coverslips with cells were washed with phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde (PARA) (Electron Microscopy Sciences, ON) in PBS for 10-15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the tops of the wells were cleaned and cells on the bottom of the membrane were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, 15713) and stained with 10 μg/mL DAPI in 1% Triton X100 (Sigma T9284) ...
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bioRxiv - Developmental Biology 2024Quote: Bovine embryos and embryonic cells from different batches/days of experiments were fixed in freshly made 4% paraformaldehyde (Electron Microscopy Science, Hatfield, PA) at room temperature for 15 minutes followed by permeabilization in 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2020Quote: ... Mounting media (~150 μL/slide) was prepared the day of use by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were subjected to intracardiac perfusion with 5mL of PBS containing heparin followed by 20mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, RT 15714) in PBS ...
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bioRxiv - Genetics 2022Quote: ... samples were washed 3x in 0.3% v/v PBT and subjected to a second sample fixation in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Genetics 2022Quote: ... adult female intestines were dissected out in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 45 min while rocking ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells grown on coverslips were washed in phosphate-buffered saline (PBS) and fixed in freshly prepared PBS supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary tumors were cut in half and either snap-frozen in dry ice or fixed with 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA). At the endpoint ...