Labshake search
Citations for Electron Microscopy Sciences :
1751 - 1783 of 1783 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
-
bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
-
bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
-
bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... This concentrate was diluted 1:2 with molecular grade water and 50 µL was fixed with 25% glutaraldehyde (stored at 4°C, Electron Microscopy Sciences, Hatfield, PA, USA) to a final concentration of 0.25% ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were stained en bloc with 2% aqueous uranyl acetate overnight at 4° C in the dark (22400-2, Electron Microscopy Sciences, Hatfield, PA, USA). After staining ...
-
bioRxiv - Genetics 2024Quote: ... i3LMNs were cultured in clear-bottom imaging 96-well plates and fixed with an equivalent volume of 4% paraformaldehyde (PFA, Electron Microscopy Sciences 50-980-487) in PBS added directly to the cell culture media ...
-
bioRxiv - Biochemistry 2024Quote: ... 4 µl of sample were added to freshly glow-discharged 300 mesh 2/1 µm C-flat copper grids (Electron Microscopy Sciences, Hatfield, Pennsylvania, USA), blotted for 10 s at blot force 4 at 100% relative humidity and 4°C before being plunge frozen in a liquid mix of 37% (v/v ...
-
bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
-
bioRxiv - Neuroscience 2022Quote: ... The slice was then left to rest in the recording chamber for 4-5 minutes to ensure transport of biocytin to distal dendrites and axon processes (Swietek et al., 2016)39 before the slice was moved into 4% PFA (Electron Microscopy Sciences, CAS #50-00-0) diluted in 0.1M Dulbecco’s Phosphate Buffered Saline (PBS ...
-
bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
-
bioRxiv - Bioengineering 2024Quote: Subcutaneous tumors were excised from flank skin including adjacent skin layers and then fixed in 4% PFA (16% PFA, Electron Microscopy Sciences, diluted in 1X PBS) at 4°C overnight on a rotator ...
-
bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
-
Cortical synaptic vulnerabilities revealed in a α-synuclein aggregation model of Parkinson’s diseasebioRxiv - Neuroscience 2024Quote: ... The slides were dried at RT and embedded in mounting media freshly prepared by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA, USA) with AF300 (Electron Microscopy Sciences #17977-25 ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the pellet was fixed in 2.5% glutaraldehyde and 4% PFA (16% stock solution, EM grade, Electron Microscopy Sciences in 0.1 M PIPES (pH 7.2)) for minimum 2h ...
-
bioRxiv - Bioengineering 2024Quote: ... Uterine horn cross sections (two per horn per mouse) were excised and fixed in a 4% (v/v) formaldehyde solution (Electron Microscopy Sciences, 32% aqueous solution, Cat# 100496-496) in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used were goat-anti-Rabbit IgG conjugated to 10nm gold particles (Electron Microscopy Sciences, Cat. # 25109). We also labeled the samples with secondary gold-conjugated antibody alone to test for the non-specific labeling and found virtually no labeling in each case (data not shown) ...
-
bioRxiv - Molecular Biology 2023Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
-
bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
-
bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: Larvae were dissected to optimally visualize the body wall muscles in ice-cold HL3.1 buffer and fixed with 4% paraformaldehyde in HL3.1 for 20 minutes for all antibodies except GluRIIA for which samples were fixed in Bouin’s fixative (Electron Microscopy Sciences, 15990) for 5 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... sections were incubated with a fluorescent-tagged secondary antibody for 45 minutes at room temperature and embedded in DAPI/Fluoromount (Electron Microscopy Sciences). All details about fixatives ...
-
bioRxiv - Neuroscience 2024Quote: ... fluorescently tagged goat anti-mouse and anti-chicken secondary antibodies were diluted in PBS and incubated for 30 min and coverslips were mounted using Fluoromount-G mounting medium (Electron Microscopy Sciences).
-
bioRxiv - Pathology 2022Quote: ... Slides were incubated at RT for an hour in secondary antibody and mounted with Fluoro-Gel II containing DAPI (Electron Microscopy Sciences, 17985). Slides were viewed using a Zeiss Axio Imager.A2 Microscope and images were taken using Axiocam 105 color (chromogenic detection ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
-
bioRxiv - Neuroscience 2024Quote: ... the grids were incubated with secondary antibodies conjugated to 10 nm or 6 nm gold particles (Cytodiagnostics, 1:20, and Electron Microscopy Sciences, 1:40) in PBS containing 0.1% gelatin at 21 °C for 1 h ...