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Citations for Electron Microscopy Sciences :
1651 - 1700 of 1755 citations for Dengue Virus Serotype 4 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Day 11 IVDi tissues were treated while still attached to the transwell member as follow: culture medium was carefully removed from the transwell and the whole membrane was fixed in 4% PFA (Electron Microscopy Science, 15713) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...
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bioRxiv - Genetics 2022Quote: ... adult female flies were dissected in ice cold 1X PBS and fixed in 4% w/v paraformaldehyde (Electron Microscopy Sciences, Cat. No. 15714) in PBS for 2 hours while rocking ...
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bioRxiv - Developmental Biology 2022Quote: ... The inner ears were dissected from P0 pups and E15.5 embryos directly into cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... mice were transcardially perfused with 20 mL ice cold 1X DPBS followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA). Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... they were perfused with 40 mL of ice-cold PBS(-) and then with 30 mL of ice-cold 4% PFA (Electron Microscopy Science 15714) in PBS ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: Quiescent media was replaced every other day for 7 days and then adherent qNSCs were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and five old (20-22 month-old) C57BL/6 male mice were subjected to transcardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, 15714) in PBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging or fixed with 4% PFA (Electron Microscopy Sciences, cat # 50980487) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of freshly purified peak fraction sample was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA), washed with deionized water and stained with freshly prepared 1.5% uranyl formate ...
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bioRxiv - Microbiology 2023Quote: Salivary glands were dissected out from wandering third instar larvae and fixed in PBS with 4% paraformaldehyde (EM Grade; Electron Microscopy Sciences, 15710) at room temperature for 20 min with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips (Menzel-Glaser) were washed twice in PBS and then fixed with 4% paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in HBSS(-) for 30 min at room temperature (RT) and then immediately fixed in 4% PFA (Electron Microscopy Sciences, #15710) in PBS for 1 hr at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... BFA was washed out by three brief rinses in PBS and cells were then incubated at 37 °C for indicated times before fixation with 4% PFA (EMS, catalog no: 15710). For the quantification of Golgi complex recovery following BFA treatment the Ilastik 1.4.OS machine learning software was used ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are washed with culture medium and fixed for 2 minutes with room temperature fixative (4% EM grade paraformaldehyde (Electron Microscopy Sciences #19202) + 0.1% EM grade glutaraldehyde (Ted Pella ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Images of fixed cells were taken on either the LSM or spinning disk confocal microscopes at 40x after incubation in 4% paraformaldehyde (Electron Microscopy Sciences, #15710) for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Bioengineering 2024Quote: ... A small drop (2.5 mL) of the protein solution was applied to the carbon side of a glow-discharged grid (EMS, CF300-CU) and left on the grid for 1 min ...
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bioRxiv - Cell Biology 2020Quote: ... proliferating MuSC and C2C12 cells were cultured on pre-coated glass coverslips and then fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences, Hatfield, PA). RNA hybridization and signal development were carried out using Basescope assay (Advanced Cell Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Cell Biology 2022Quote: Roughly 1 × 106 trypsinized cells in flow buffer (HBS with 1% BSA) were fixed using 4% paraformaldehyde (cat no. 15710; Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2019Quote: ... NaNO2 (# S25560) and NaCl (# 7647-14-5) which is followed by perfusion with 40 mL of cold 4% paraformaldehyde (Electron Microscopy Sciences, #RT-15714) in 1X-phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Mounting media (~150 μL/slide) was prepared the day of use by mixing Tris-MWL 4-88 (Electron Microscopy Sciences #17977-150, Hatfield, PA) with AF300 (Electron Microscopy Sciences #17977-25 ...
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bioRxiv - Developmental Biology 2019Quote: Mice were sacrificed according to ethical guidelines and inner ears were dissected out in cold PBS and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, cat: 15710) for 2 hrs at room temperature ...