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Citations for Electron Microscopy Sciences :
1651 - 1700 of 1764 citations for 8 CHLORO 2 METHYL IMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and cover-slipped with ThermoFisher Gold Seal Cover Glass 24 x 60mm No.1 thickness (Electron Microscopy Sciences, 63770-01). Sections were imaged using a Leica CTR6000/B microscope to quantify sulfatide accumulation in different brain areas.
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... pelleted and washed with PBS before being resuspended in 1 mL of 4% PFA (Electron Microscopy Sciences 30525-89-4) + 0.0075% glutaraldehyde (Sigma G6257 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated in 0.1 M sodium cacodylate buffer supplied with 1% OsO4 and 0.8% potassium ferricyanide (Electron Microscopy Sciences), for 2 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Neuroscience 2023Quote: ... iAssembloids were first fixed with 4% paraformaldehyde (16% stock, diluted 1:4 in DPBS, Electron Microscopy Sciences, Cat. No. 15710) and then incubated in a 25% formamide (MilliporeSigma ...
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bioRxiv - Bioengineering 2023Quote: Purified DNA origami structures (1–10 nM) were adsorbed onto freshly glow discharged copper grids (Electron Microscopy Sciences, Hatfield, PA) and incubated for 4 minutes ...
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bioRxiv - Genetics 2023Quote: The two epididymides of mature males were recovered in 1 mL of 0.1 M sodium cacodylate buffer (pH 7.4, Electron Microscopy Sciences) and the sperm were allowed to swim for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... transcardial perfusion was performed using 1× phosphate-buffered saline (PBS) and 4% paraformaldehyde (PFA; Electron Microscopy Sciences, the United States). The mouse liver and kidney were dissected and postfixed in 4% PFA for 6 hours at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... an overlay of rat tail collagen type 1 solution (2.5 mg/mL) in NF medium (or EMS for disease conditions) was deposited on top of the hepatocytes inside the borders of the oval-well ...
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bioRxiv - Neuroscience 2024Quote: ... 500ul 1% neutral red dye in PBS was injected intraperitoneally 2 hours prior to cardiac perfusion with ice cold fixation buffer (2.5% glutaraldehyde, 1% paraformaldehyde, in 0.2 M sodium cacodylate buffer (Electron Microscopy Sciences). Lesion tissues were then dissected out and cut into 2mmx2mm sections ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of culture was sampled (Fig. S1) and chemically fixed by adding paraformaldehyde (PFA; Electron Microscopy Science; EM grade) to a final concentration of 2% ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Systems Biology 2020Quote: ... rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Systems Biology 2020Quote: ... the La2-DAB solution was removed and cells were rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Cell Biology 2019Quote: ... sulfated proteoglycans were fixed in 10% formalin and stained for 30 minutes with 1% Alcian Blue 8GS solution (Electron Microscopy Sciences) on day 12 after plating ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were allowed to fix overnight at 4 °C and then post-stained with 1% aqueous osmium tetroxide (EMS, 19100) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen samples were then opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS, USA) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with cold PBS for 1 minute and then fixed with 4% PFA (Electron Microscopy Sciences: 157-4) for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... infiltrated with Embed-812 resin series by 20% increments in acetone (each step was 1 h) and embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60°C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Bioengineering 2023Quote: ... A 3-μL droplet of the L/D-GQD solution (0.1 mg·mL-1) was placed on the carbon-coated copper TEM grid (CF200-Cu-25; Purchased from Electron Microscopy Sciences, PA) and allowed to dry in the air ...
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bioRxiv - Developmental Biology 2023Quote: Wing imaginal discs dissected from late third instar larvae in 1×PBS (pH 7.4) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 40[minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Bioengineering 2024Quote: A 20 µL droplet of 10 nM origami solution diluted in 1×TAE buffer sup- plemented with 12.5 mM MgCl2 was deposited onto a freshly cleaved mica substrate (Electron Microscopy Sciences). The sample was incubated for 1 minute ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mm diameter PDMS wells were passively adhered to #1 9 mm x 9 mm glass coverslips (Electron Microscopy Sciences #72190-09). Wells were passivated overnight using vapor deposition of Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931 ...
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bioRxiv - Cell Biology 2019Quote: For negative staining 3 µl of purified 40S+60S ribosome fractions or alternatively 3 µl of 40S+60S ribosome fractions in the presence of (PR)20 (1:10 molar ratio) were deposited onto freshly glow-discharged carbon-coated 400 mesh copper electron microscopy (EM) grids (Electron Microscopy Sciences) and retained on the grid for 1 min ...
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bioRxiv - Neuroscience 2019Quote: ... and then immersed in 50% ethanol before being dehydrated in ascending concentrations of ethanol (50%, 70%, 90%, 100%) containing 1% uranyl acetate (UA; Electron Microscopy Sciences) with application of microwave irradiation (250 W ...