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Citations for Electron Microscopy Sciences :
1601 - 1650 of 1743 citations for 6 Methylchroman 4 ylamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Cat# H-12-1.5-PLL) and fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The animals were then transcardially perfused with PBS and then with 4% paraformaldehyde (PFA) (diluted into 1xPBS from 16% PFA, Electron Microscopy Sciences), followed by post-fixation with 4% PFA for immunohistochemistry or Karlsson and Schultz (KS ...
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bioRxiv - Neuroscience 2024Quote: Mice were placed under isoflurane anesthetics until loss of pain reflex and transcardially perfused with filtered ice-cold PBS then 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were cryoprotected in 30% sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Neuroscience 2024Quote: P14 mice were euthanized and perfused transcardially with ice-cold Phosphate Buffered Saline (PBS) followed by ice-cold 4% paraformaldehyde (Electron Microscopy Sciences) diluted in water ...
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: Mice were deeply anesthetized with a mixture of Ketamine/Xylazine and perfused transcardially with saline followed by 4% paraformaldehyde (PFA; Electron Microscopy Science). Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on 35-mm Glass Based Dishes which have a cover glass with a grid pattern (IWAKI) and fixed after laser-microirradiation with 4% PFA (Electron Microscopy Sciences) containing 0.1% Triton X-100 (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibody incubation was followed by 4 x 15-minute washes in 1X PBS and slide mounting using Fluor-Gel mounting medium (Electron Microscopy Sciences). Tissues were mounted epithelium-side up.
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bioRxiv - Biochemistry 2024Quote: To generate negative-stain grids 4 µl of the GraFix fraction was applied to a Copper 400 Mesh grids with continuous carbon (Electron Microscopy Sciences) after glow discharge ...
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bioRxiv - Cell Biology 2024Quote: ... were immersed in and fixed by 4 % glutaraldehyde (48 h/4 °C), post-fixed by 1% osmium tetroxide (2 h, RT) and were embedded into Epon resin (Electron Microscopy Sciences) as described previously32 ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Developmental Biology 2023Quote: ... Adherent cells (at ESC stage for the CRISPRa validations and PP stage for the CRISPRi validations) were rinsed once with PBS with Ca2+ and Mg2+ (PBS++) and fixed with 4% Paraformaldehyde (Electron Microscopy Sciences 32% Paraformaldehyde ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice in warm 1X HBSS and fixed for 15min in 4% paraformaldehyde (PFA) made from fresh 16% PFA (#15710, Electron Microscopy Sciences) diluted with 1X HBSS ...
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bioRxiv - Bioengineering 2023Quote: Rats implanted with MEV probes were anaesthetized with 3% isoflurane or 300 mg kg−1 of ketamine (Patterson Veterinary Supply) and transcardially perfused with ice-cold 30 ml saline and 30 ml 4% paraformaldehyde (PFA, Electron Microscopy Sciences) at specified times post-injection ...
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bioRxiv - Neuroscience 2023Quote: ... the monkeys were deeply anesthetized (ketamine, 10 mg/kg and sodium pentobarbital, 100 mg/kg) and transcardially perfused with 4% formaldehyde (EMS, Hatfield, PA) in Phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with PBS (containing CHX for cells treated with it) and fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS for 10 min at RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and xylasine (10 mg/kg) and then intracardially perfused with ice-cold PBS and ice-cold 4% paraformaldehyde dissolved in PBS (PFA) (Electron Microscopy Sciences). Brains ...
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bioRxiv - Genetics 2023Quote: Testis and epididymides samples from 8-16-week-old mice were fixed for 24 h in PBS/4% PFA (Electron Microscopy Sciences), dehydrated in a graded ethanol series ...
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bioRxiv - Developmental Biology 2023Quote: For wholemount immunofluorescent labeling heads were bisected and brains removed prior to immersion fixation of the intact inner ear and skull in 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences) prepared in 67mM Sorensons phosphate buffer (pH 7.4) ...
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bioRxiv - Cell Biology 2023Quote: Cells seeded in 24-well plates on poly-D-Lysine-coated glass coverslips were washed once in PBS and fixed with 4 % methanol-free PFA (Electron Microscopy Science) for 15 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... the sample holder was removed from the microscope in order to perform sample fixation using 4 % PFA (Catalog no. 15714, Electron Microscopy Sciences) and 0.08 % glutaraldehyde (Catalog no ...
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bioRxiv - Bioengineering 2023Quote: Mice with implanted mesh probes were anaesthetized and transcardially perfused with ice-cold 40 ml PBS and 40 ml 4% formaldehyde (Electron Microscopy Sciences) at specified times post-implantation ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... and xylazine (60 mg/kg) then transcardially perfused with 10mL of PBS and 40mL of freshly hydrolyzed 4% paraformaldehyde (19210, Electron Microscopy Sciences). Tissues were gently dissected and placed in 4% paraformaldehyde for post fixation ...
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bioRxiv - Cell Biology 2024Quote: Mice of desired age and genotype were sacrificed by cervical dislocation and their eyes were immediately collected and immersed in 4% PFA in PBS (n°15714S, Electron Microscopy Sciences). They were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and transcardial perfusion was performed with 10 mL of 1x PBS followed by 10 mL of ice cold 4% PFA (EMS, #15710-S) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with pentobarbital (Fatal-Plus, Vortech Pharmaceuticals) and intracardially perfused with ∼50 ml of 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature (25°C) with 4% PFA (Electron Microscopy Sciences) diluted in PBS and stored at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: mESCs derived MNs were plated on pre-coated 12 mm diameter coverslips and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 200-071, Virbac) and transcardially perfused with 30mL of PBS (BP399, Fisher bioreagents) followed by 50mL of 4% PFA (18210, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Biochemistry 2024Quote: Cryo-grids were made by evaporating a 20 nm gold layer with ∼4 µm holes on the rhodium side of 400-mesh copper-rhodium grids (Electron Microscopy Sciences) in a Leica EM ACE200 Vacuum Coater ...