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Citations for Electron Microscopy Sciences :
1601 - 1650 of 2511 citations for 6 Fluoro 4 hydroxy 1 7 naphthyridine 3 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Cells on 96-well glass bottom plates were washed in PBS0 and fixed in 4% formaldehyde (Electron Microscopy Sciences) for 5 min at RT ...
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bioRxiv - Neuroscience 2020Quote: Mice were sacrificed and transcardially perfused with cold PBS with heparin followed by cold 4% PFA (Electron Microscopy Sciences). The intact brain was separated carefully from the skull and placed in 4% PFA ...
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bioRxiv - Cell Biology 2019Quote: ... prepared in BRB80 buffer (80 mM PIPES pH 6.9, 1mM MgCl2, 1mM EGTA) or 4% prewarmed paraformaldehyde (EMS, 15170) in PBS for 10 min or 20 min respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... all WMISH fixations were done in 4% paraformaldehyde (PFA, stock 32% PFA ampules from Electron Microscopy Sciences, cat. 15714) in ASW for 6 h–overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... whole globes dissected from embryonic 13-LGS were immersed in freshly prepared cold 4% paraformaldehyde (Electron Microscopy Sciences, USA) in 0.1M sodium phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were excised and fixed for 10 min in 4% (w/v) paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: N2 worms were mutagenized for 4 h by incubation in the presence of 0.05 M ethyl methane sulfonate (EMS) according to the standard protocol (24) ...
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bioRxiv - Synthetic Biology 2022Quote: ... or Ang1 at 100 nM of F-domain concentration for 15 min before fixing with 4% PFA (EMS, 15710) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were then removed with curved surgical scissors and placed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Catalog#: IC993M31) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat. no. 19100 Electron Microscopy Sciences) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed once with PBS+ and then fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences, 15713) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Genetics 2023Quote: ... coverslips were washed with PBS and fixed at RT for 10 minutes with 4% PFA (Electron Microscopy Sciences, 15713S). After fixation ...
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bioRxiv - Cell Biology 2023Quote: Embryonic tissues for cryosections were fixed for 2h in 4% paraformaldehyde (PFA) in PBS (Electron Microscopy Sciences, Cat #:15710) with 0.5% Triton X-100 at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested by centrifugation and fixed at room temperature while nutating in 4% paraformaldehyde (Electron Microscopy Sciences #15710). Cells were washed with PBS with 2mM EDTA and permeabilized at 45°C with 2% w/v sodium lauroyl sarcosine (Bioworld #41930024-3 ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells on transwell filters in well plates were fixed with 4% paraformaldehyde (#15710, Electron Microscopy Sciences, Hatfield, PA, USA) in PBS for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: ... all WMISH fixations were done in 4% paraformaldehyde (PFA, stock 32% PFA ampules from Electron Microscopy Sciences, cat. 15714) in ASW for 6 h–overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... a 4% (v/v) paraformaldehyde (PFA) solution was diluted from 16% stock (Cat# 50-980-487, Electron Microscopy Sciences) by 1X Gibco™ DPBS (Cat# 14190-136 ...
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bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
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bioRxiv - Immunology 2024Quote: ... Neurons were washed once with 500ul of cold PBS and fixated with PBS + PFA (4:100, Electron Microscopy Sciences) for 10 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: Seeds of the pen3-4 (Stein et al., 2006) mutant were mutagenized by treatment with 0.24% ethyl-methansulfonate (EMS) for 16h (Normanly et al. ...
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bioRxiv - Neuroscience 2024Quote: ... the dams were then myocardially perfused with ice-cold 1X PBS and ice-cold 4% PFA (Electron Microscopy Sciences, 20% solution ...
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bioRxiv - Developmental Biology 2024Quote: ... and fixed by immersion for at least one overnight at 4°C in 2.5% glutaraldehyde (Electron Microscopy Sciences; 16200) and 4% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Bioengineering 2024Quote: ... before being fixed in pre-warmed (5 min in 37° C water bath) 4% formaldehyde (Electron Microscopy Sciences, 15710) in PBS solution for 5 min and permeabilized in pre-warmed (to 37° C ...
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bioRxiv - Neuroscience 2024Quote: ... Then the tissue was homogenized at ∼500 rpm for 4 strokes using an overhead stirrer (Electron Microscopy Sciences 6480610) and a tissue grinder (Electron Microscopy Sciences 6479310 ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Neuroscience 2019Quote: ... and then placed in 1:1 propylene oxide:EMBed resin (EMS) rotating at room temperature overnight ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were dissected in IMADS (Singleton and Woodruff 1994) or 1X PBS and fixed with 4% PFA (Electron Microscopy Sciences) in 1X PBS plus 0.3% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were first washed with PBS for 5 minutes and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, PA, USA) in PBS containing 0.1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... samples were washed once in wash buffer and fixed with a 4% Paraformaldehyde + 0.1% Glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) solution made in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected larvae were fixed for 20 (VNC) or 45 minutes (NMJs) in 4% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA) in phosphate-buffered saline (PBS) ...