Labshake search
Citations for Electron Microscopy Sciences :
1601 - 1650 of 2488 citations for 4 1 Hydroxymethyl cyclopropyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion with phosphate-buffered saline (PBS, Quality Biological, 119-069-491) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) was performed immediately after ...
-
bioRxiv - Cancer Biology 2023Quote: ... MFPs were fixed in cold 2% v/v glutaraldehyde with 4% paraformaldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) for 4 hr followed by one wash in the same buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
-
bioRxiv - Bioengineering 2023Quote: Fixation was done by replacing half the volume of media in the culture dish with 4 % paraformaldehyde (Electron Microscopy Sciences; 15714) in phosphate buffered saline (PBS ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
-
bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tissue for analysis of cytosolic expression of GFP or tdTomato was directly fixed in ice-cold 4% PFA (Electron Microscopy Science) for 2 h at 4° ...
-
bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
-
bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... cerebellums were harvested first and then the same mice were the perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in PBS for 15 min ...
-
bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
-
bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
-
bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
-
bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were washed with cold PBS supplemented with 0.1 mM CaCl2 and 2 mM MgCl2 (PBSCM) and fixed in 4% paraformaldehyde (Electron Microscopy Sciences)/4% sucrose for 8 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... Whole kidney organoids and mouse kidney explants were fixed for 20 min on ice in 4% PFA (Electron Microscopy Sciences, 15710) in 1xPBS ...
-
bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with ice-cold phosphate buffer saline (PBS) followed by PBS containing 4% paraformaldehyde (PFA) (Electron Microscopy Sciences). Brains were extracted ...
-
bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... then fixed for 20 minutes at room temperature (RT) in a fixative comprised of 4% (v/v) paraformaldehyde (Electron Microscopy Sciences), 25% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... either immediately snap-freezing in tubes on dry ice or being immediately immersed in 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) diluted in PBS for fixation overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then transferred into an ice filled bath and washed 4× for 30 min each with acetone and infiltrated with Spurr’s resin (Electron Microscopy Sciences). During final steps of resin infiltration ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Tissues were pooled and fixed in a fixation buffer (100mM Tris pH7.5, 300mL NaCl, 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA)) for 20min on a rotation platform set to a gentle speed at a frequency of 0.33Hz ...
-
bioRxiv - Genomics 2024Quote: We selected the 5861 promoters accessible in the somatic cells of 4-cell stage embryos (earlyEMS, EMS and ABx cell types), and measured and visualised the ATAC-seq signal from adult germline cells (Serizay et al ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then moved to a 1:1 solution of liquid nitrogen and 1% uranium acetate (Electron Microscopy Sciences, 22400) before undergoing freeze substitution and low temperature embedding (Leica ...
-
bioRxiv - Neuroscience 2021Quote: ... and then post-fixed for 1 hr in 1% osmium tetroxide (EMS) in 0.1 M sodium cacodylate ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
-
bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... infected or treated as noted for each experiment and fixed for 10 minutes at room temperature in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences) in PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
-
bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... cells were fixed for 10 or 30 (if infected with SARS-CoV-2) min in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences).
-
bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: Cells were grown on 35 mm Glass Based Dishes or 24-well EZVIEW Glass Bottom Culture Plates LB (IWAKI) and fixed with 4% PFA (Electron Microscopy Sciences) for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were fixed using 4% paraformaldehyde (Serva, Heidelberg, Germany) and 2% glutaraldehyde (Serva) in PBS followed by staining with 0.5% osmium tetroxide (EMS, Hatfield, PA, USA). After thorough rinsing in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
-
bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen (invadopodia formation assay) or gelatin-coated coverslips (degradation assay) and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
-
bioRxiv - Bioengineering 2021Quote: ... The tissue-HDMI-array sandwich was moved to room temperature, and the tissues were fixed in 4% formaldehyde (100 μl, diluted from the EM-grade 16% paraformaldehyde (#15170, Electron Microscopy Sciences)) for 10 min.
-
bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... pH 7.4) and placed on a 6-mm diameter chamber made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5-mm clean coverglass (EMS # 72200-31 ...