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Citations for Electron Microscopy Sciences :
1551 - 1600 of 3000 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Media was aspirated and cells were washed with PBS at RT once before being incubated with 4% PFA (Electron Microscopy Sciences) made up in PBS with the addition of 4% sucrose at RT for 15 mins ...
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bioRxiv - Cell Biology 2022Quote: ... high parasitemia culture was pelleted by centrifugation (1.5 g, 3min, RT) and resuspended in freshly prepared PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) and 0.0075% gluteraldehyde (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized and transcardially perfused with 0.1M phosphate buffer (PBS) followed by 4% EM-grade paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were dissected and post-fixed overnight in 4% PFA ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were fixed for 15 min at RT with 4% electron microscopy grade paraformaldehyde (PFA; Electron Microscopy Sciences, cat. no. 15710) diluted in PIPES–EGTA– magnesium (PEM ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was removed and the neurons were fixed with 4% electron-microscopy-grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in a cytoskeleton-protective buffer (PEM ...
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bioRxiv - Cell Biology 2020Quote: ... a sucrose gradient was created using 2.3 ml of a 20 % sucrose solution containing 15 mM HEPES [pH 7.4] and 4 % paraformaldehyde aqueous solution (EM Grade) (Electron Microscopy Sciences) and 2 ml of a 10% sucrose solution containing 15 mM HEPES [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Monolayers were fixed and plaques visualized with a solution of 0.4% crystal violet by weight in 80% methanol (MilliporeSigma) and 4% PFA (Electron Microscopy Sciences) for 20 min at room-temperature (RT).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: Samples (4 μL) were applied to glow-discharged continuous carbon film supported copper grids (CF300-CU-50 grids, Electron Microscopy Sciences). After 1 min incubation ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were rinsed in 1x PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl2) pH 6.8 and fixed in 4% formaldehyde (FA, #15710, EMS, Hatfield, USA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion with phosphate-buffered saline (PBS, Quality Biological, 119-069-491) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) was performed immediately after ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: Fixation was done by replacing half the volume of media in the culture dish with 4 % paraformaldehyde (Electron Microscopy Sciences; 15714) in phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue for analysis of cytosolic expression of GFP or tdTomato was directly fixed in ice-cold 4% PFA (Electron Microscopy Science) for 2 h at 4° ...
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bioRxiv - Microbiology 2022Quote: Monolayers of HFF infected with Toxoplasma from 6 or 24 h were fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, PA) in 0.25 M HEPES (ph7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... cerebellums were harvested first and then the same mice were the perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in PBS for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole kidney organoids and mouse kidney explants were fixed for 20 min on ice in 4% PFA (Electron Microscopy Sciences, 15710) in 1xPBS ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with ice-cold phosphate buffer saline (PBS) followed by PBS containing 4% paraformaldehyde (PFA) (Electron Microscopy Sciences). Brains were extracted ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... then fixed for 20 minutes at room temperature (RT) in a fixative comprised of 4% (v/v) paraformaldehyde (Electron Microscopy Sciences), 25% (v/v ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... either immediately snap-freezing in tubes on dry ice or being immediately immersed in 4% paraformaldehyde (Electron Microscopy Sciences, 15714-S) diluted in PBS for fixation overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then transferred into an ice filled bath and washed 4× for 30 min each with acetone and infiltrated with Spurr’s resin (Electron Microscopy Sciences). During final steps of resin infiltration ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tissues were pooled and fixed in a fixation buffer (100mM Tris pH7.5, 300mL NaCl, 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA)) for 20min on a rotation platform set to a gentle speed at a frequency of 0.33Hz ...
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bioRxiv - Genomics 2024Quote: We selected the 5861 promoters accessible in the somatic cells of 4-cell stage embryos (earlyEMS, EMS and ABx cell types), and measured and visualised the ATAC-seq signal from adult germline cells (Serizay et al ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Microbiology 2021Quote: ... infected or treated as noted for each experiment and fixed for 10 minutes at room temperature in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown on 35 mm Glass Based Dishes or 24-well EZVIEW Glass Bottom Culture Plates LB (IWAKI) and fixed with 4% PFA (Electron Microscopy Sciences) for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...