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Citations for Electron Microscopy Sciences :
1501 - 1550 of 1782 citations for 6 Chloro 3H imidazo 4 5 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue for analysis of cytosolic expression of GFP or tdTomato was directly fixed in ice-cold 4% PFA (Electron Microscopy Science) for 2 h at 4° ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Neuroscience 2023Quote: ... cerebellums were harvested first and then the same mice were the perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, PA) in PBS for 15 min ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were rinsed in 1x PHEM buffer (60mM PIPES, 25mM HEPES, 10mM EGTA, 2mM MgCl2) pH 6.8 and fixed in 4% formaldehyde (FA, #15710, EMS, Hatfield, USA) and 0.1% glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2023Quote: ... The block edges were covered with additional strips of carbon tape and 2-4 strips of copper tape (EMS; cat#77802) and coated with a layer of colloidal silver liquid (EMS ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were euthanised with an overdose of MS-222 (1g/L in seawater) and fixed in 4% paraformaldehyde (Electron microscopy sciences) as per Gillis et al ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... 20 uL of BEV sample was fixed in 2% EM-grade paraformaldehyde (157-4-100; Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were washed with cold PBS supplemented with 0.1 mM CaCl2 and 2 mM MgCl2 (PBSCM) and fixed in 4% paraformaldehyde (Electron Microscopy Sciences)/4% sucrose for 8 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion with phosphate-buffered saline (PBS, Quality Biological, 119-069-491) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) was performed immediately after ...
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bioRxiv - Developmental Biology 2023Quote: Wing imaginal discs dissected from late third instar larvae in 1×PBS (pH 7.4) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 40[minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... mouse tissues were perfused with 10 mL of cold PBS and 5mL of 4%paraformaldehyde (PFA) (Electron Microscopy Sciences, CAT# 15710). Brains were post-fixed overnight in 4% PFA at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... MFPs were fixed in cold 2% v/v glutaraldehyde with 4% paraformaldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) for 4 hr followed by one wash in the same buffer ...
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bioRxiv - Microbiology 2023Quote: Mice lungs were perfused with 8 mL PBS and inflated with 0.7 mL of OCT compound (Tissue-Tek) mixed 1:1 v/v with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were diluted to 0.01 mg/mL and 4 μL was applied to a glow discharged Formvar/Carbon grid (Electron Microscopy Sciences) for 30 s ...
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bioRxiv - Neuroscience 2023Quote: Animals were deeply anesthetized with ketamine/xylazine and transcardially perfused with 5-10 mls of 37°C 0.9% sterile saline followed by 10 mls of room temperature 4% EM Grade paraformaldehyde (PFA, Electron Microscopy Sciences) in 0.9% saline.
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bioRxiv - Bioengineering 2023Quote: Fixation was done by replacing half the volume of media in the culture dish with 4 % paraformaldehyde (Electron Microscopy Sciences; 15714) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... or 30 min and fixed by adding an equal volume of 4 % pre-warmed paraformaldehyde (Electron Microscopy Sciences, final concentration 2 %), followed by 20 min incubation at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and washed again twice for 5 minutes each in PBS before coverslipping with Fluoro-Gel (#17985, Electron Microscopy Sciences). Primary antibodies used include ...
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bioRxiv - Cell Biology 2019Quote: ... homozygous pans1-1 seeds were incubated for 17 h at room temperature in 5 mL of 0.3% (v/v) ethyl methanesulfonate (EMS) (SIGMA ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 µl of this diluted sample was immediately applied onto glow-discharged grids (Electron Microscopy Sciences, CF400-Cu). The samples were stained with 2% uranyl acetate ...
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bioRxiv - Biochemistry 2019Quote: 5 µl of TorsinA filaments or TorsinA-liposome mixtures were loaded on glow-discharged (EMS 100, Electron Microscopy Sciences) continuous carbon film grids (CF200-Cu ...
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bioRxiv - Biochemistry 2019Quote: 5 µl of TorsinA filaments or TorsinA-liposome mixtures were loaded on glow-discharged (EMS 100, Electron Microscopy Sciences) continuous carbon film grids (CF200-Cu ...
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bioRxiv - Cell Biology 2019Quote: ... samples of 1-2 mm thickness were fixed through immersion in 5% (w/v) glutaraldehyde (Biological Grade, EMS, #16500) in 0.05M phosphate buffer pH 7.3 for 24 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples (5 μL) were loaded onto a glow discharged Formvar/carbon-coated copper grid (400 mesh, Electron Microscopy Sciences) and stained with 2% uranyl formate ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was immersed for 5 min in reduced osmium solution containing 1% osmium tetroxide (OsO4; Electron Microscopy Sciences) and 1.5% potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently mounted on glass slides in Citifluor Mountant Solution (Electron Microscopy Sciences, cat no. AF100-5 & 17977-150). Slides were imaged on a W1-Tokogawa spinning disk confocal microscope using a Nikon 100x ...
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bioRxiv - Biophysics 2023Quote: ... Grids were picked up from the glass bottom MatTek dish using 5/15 Style Dumont Tweezers (Electron Microscopy Sciences), transferred to their respective tweezers and placed into the EM GP chamber set at 25°C and 99% humidity or the CP3 chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed at 10-15 min post-damage for 45 min with 4% paraformaldehyde (PFA; Cat No – RT-15710, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Microbiology 2021Quote: ... infected or treated as noted for each experiment and fixed for 10 minutes at room temperature in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences) in PBS ...
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bioRxiv - Microbiology 2019Quote: ... plasmids encoding the DENV protein variants were transfected individually into human HEK-293T cells and allowed to express for 22 h before fixing cells in 4% paraformaldehyde (Electron Microscopy Sciences), and permeabilizing with 0.1% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed for 10 or 30 (if infected with SARS-CoV-2) min in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences).
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...