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Citations for Electron Microscopy Sciences :
1501 - 1550 of 2293 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Akorn) and perfused transcardially with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Embryos were decapitated and heads were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... After 24 hours, fibroblasts were fixed with 4% (w/v, in PBS) paraformaldehyde (Electron Microscopy Sciences 15710), permeabilized with 0.1% (w/v ...
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bioRxiv - Immunology 2022Quote: Brains were collected after transcardial perfusion with ice-cold PBS followed by 4% PFA (Electron Microscopy Sciences). Collected brains were post-fixed with 4% PFA (4 □ ...
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bioRxiv - Neuroscience 2022Quote: ... Samples fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Neuroscience 2023Quote: ... immediately followed by 4% paraformaldehyde (PFA; diluted in PBS from a 16% stock solution; Electron Microscopy Sciences). The brain was then removed and transferred into PFA solution overnight before being thoroughly rinsed and stored in PBS at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of sample was applied to a freshly plasma cleaned Ultrafoil grid (Electron Microscopy Sciences, EMS). Samples were blotted for 4s using a blot force of 20 followed by vitrification using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of sample was applied to a freshly plasma cleaned Ultrafoil grid (Electron Microscopy Sciences, EMS). Samples were blotted for 4s using a blot force of 20 followed by vitrification using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: 4 µL sample was applied to a glow-discharged grid (Electron Microscopy Sciences Formvar/Carbon 200 Mesh) for 30 sec ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a 4% PFA solution made in PBS (Electron Microscopy Sciences, 15710, Hatfield, PA) for 15 min at room temperature and then washed three times (5 min each ...
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bioRxiv - Cell Biology 2023Quote: Ovaries were (1) dissected in 1X PBS and fixed in 4% EM-grade formaldehyde (Electron Microscopy Sciences 16% Paraformaldehyde Aqueous Solution ...
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bioRxiv - Molecular Biology 2023Quote: MDCKII cells were fixed with 4 % paraformaldehyde (cat. No. 15713-S, Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: Bidimensional (2D) cultures: All cells were fixed in 4% (w/v) paraformaldehyde (Electron Microscopy Sciences, Catalog # 15712) for 10 minutes in the imaging plates ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were fixed with 4% PFA (diluted in PBS from 16% stock, Electron Microscopy Sciences) at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were transcardially perfused initially with 0.1 M PBS followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in 0.1 M PBS solution ...
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bioRxiv - Microbiology 2024Quote: ... cells were fixed using 4% paraformaldehyde (PFA) diluted from 20% PFA (Electron Microscopy Science, 50-980-493) in PBS 1X ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated overnight with syringe-filtered 4% aqueous uranyl acetate (Electron Microscopy Sciences, en-bloc step). Dehydration was achieved through an increasing ethanol series (10%– 30%–50%–70%–90%–100% ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed with 2% paraformaldehyde (PFA) (EMS cat#: 15710; 1:1 4% PFA to spent media) 10 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2024Quote: ... Coverslips were washed with PBS and fixed with 300 µl of 4% Paraformaldehyde (PFA, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... perfusion continued with a 4% paraformaldehyde (PFA; 15714-S, Paraformaldehyde 32% Solution EM grade, Electron Microscopy Sciences) fixative for 7 min ...
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bioRxiv - Neuroscience 2024Quote: ... growth media was aspirated and replaced with warm 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: Animals were sacrificed via transcardial perfusion with 1X Phosphate Buffer (PB) and 4% paraformaldehyde (Electron Microscopy Sciences). Brains were extracted and kept in paraformaldehyde for up to 24 hrs before transfer into PBS ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed in FACS buffer and fixed in PBS containing 4% paraformaldehyde (Electron Microscopy Sciences). Cells were stored at 4°C in the dark until acquisition ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed twice with cold PBS and fixed in 4% PFA (Electron Microscopy Sciences; Cat #15714) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: iPSC-derived neurons plated on coverslips were washed and fixed with 4% paraformaldehyde (Electron Microscopy Sciences, 15710) for 20 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS before being fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences 15710) in PBS for 15-20 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: Cells were fixed with 4% paraformaldehyde (in 1x PBS, diluted from 16% paraformaldehyde, Electron Microscopy Sciences 15710) for 20 minutes at room temperature and quenched with 100 mM ammonium chloride (Sigma A9434-500G ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... the flame sealed end of the tubing was immediately dipped into a parlodion solution (∼3 cm2 parlodion, Electron Microscopy Sciences [Hatfield ...
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 7 and 8) or DNA stained with Hoechst 33258 and mounted on glass slides using Fluoromount G (Electron Microscopy Sciences; Figs. 5, 6E, 6G, S1). APEX enzymes and YFPSMO were detected by GFP and YFP fluorescence ...
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bioRxiv - Plant Biology 2022Quote: ... from −30 °C to 0° in 6 h) This was followed by washing in acetone and then infiltration in Spurr resin (EMS, Hatfield, PA, US) at graded concentration and temperature (30% for 10 h from 0 °C to 20°C ...
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bioRxiv - Biophysics 2021Quote: ... then incubated in Alexa647 conjugated fAb prior to fixation in 4% PFA and 0.1% gluteraldehide (Electron Microscopy Sciences), as described previously 18 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were rinsed in PBS and fixed in a 4% paraformaldehyde solution (Electron Microscopy Sciences cat. no. 15710) for 20 minutes at room temperature ...