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Citations for Electron Microscopy Sciences :
101 - 150 of 156 citations for InP ZnS PEG NH2 Quantum Dots 640 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on studs using silver paint and coated with 5 nm of Palladium (sputter coater EMS150TS; Electron Microscopy Sciences). Samples were imaged at 5 kV on a FEI Magellan 400 XHR Field Emission Scanning Electron Microscope at the Stanford Nano Shared Facilities.
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bioRxiv - Physiology 2023Quote: ... the sections were incubated in donkey anti-sheep IgG conjugated with 0.8 nm colloidal gold (Electron Microscopy Sciences, Fort Washington, PA) diluted at 1:100 and biotinylated anti-rabbit IgG diluted at 1:500 in PBS containing 0.1% cold water fish gelatin and 1% BSA overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Negative stain TEM was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
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bioRxiv - Microbiology 2024Quote: ... Parasites 8 h after induction of the knock sideways (with 250 nM rapalog) and the control cells grown without rapalog were Percoll-enriched and fixed with 2.5 % glutaraldehyde (Electron Microscopy Sciences) in 50 mM cacodylate buffer pH 7.4 for 1 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... was carried out on the various samples with 200-mesh gold grids coated with extra thick (25-50 nm) formvar-carbon film (EMS, USA) made hydrophilic by glow discharging at 5 mA for 60 s ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Microbiology 2024Quote: ... The phage lysates were absorbed onto glow discharged forever and carbon-coated copper grids (300 nm mesh size, Electron Microscopy Sciences) at room temperature for 5 min ...
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bioRxiv - Plant Biology 2024Quote: A series of semi-thick sections (250 nm) were collected on copper slot grid (Cat. No. GS2010-Cu, Electron Microscopy Sciences). After post-staining and gold particle coating ...
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bioRxiv - Biophysics 2022Quote: ... The void peaks from each purification were frozen similarly at the eluted concentration after dilution 1:1 with 6 nm BSA-tracer gold (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2020Quote: ... Each coverslip was mounted on a stub with carbon tape and samples were sputter coated with iridium at 10μA to a thickness of approximately 5 nm (EMS model 150T S). The coverslips were then examined on a Hitachi S4300 SEM (Hitachi High Technologies America Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... washed and incubated for 1 h at room temperature with gold conjugated (10-nm) goat anti-rabbit-IgG (1:15, Electron Microscopy Sciences).
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... Retina ultrathin (60 nm) sections were cut and transferred onto slot grids with carbon-coated Formvar film (Electron Microscopy Sciences, Hatfield, PA) and poststained with uranyl acetate followed by lead citrate ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Very small small sections of 80 nm thickness were collected on a cupper grid of 200 mesh (pore size, Electron Microscopy Sciences) and stained with lead citrate for 3 min and evaluated for utrastructural analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were cut at 180 nm and mounted on 15 mm glass coverslips coated with a Tissue Capture Pen (EMS, 71314-10). Sections were then embedded in VectaShield (Vector Laboratories) ...
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bioRxiv - Immunology 2020Quote: ... Ultrathin 70-nm sections were produced with a Leica EM UC6 ultramicrotome and mounted on copper–Formvar–carbon grids (Electron Microscopy Sciences). Observations were made on a Tecnai 10 electron microscope (FEI ...
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bioRxiv - Neuroscience 2021Quote: ... was applied to glow-discharged Quantifoil holey carbon grids (Gold R2/1; 200 mesh) overlaid with an additional 2-nm carbon layer (Electron Microscopy Sciences). The grids were blotted for 3-5 s and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... the grids were incubated in a drop of Goat-anti-Mouse IgG coupled with 10 nm gold nanoparticles (Electron Microscopy Sciences, 25512) diluted at 1:100 in PBS containing 0.1% BSA for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Cell Biology 2022Quote: ... The free face was then coated with carbon (20 nm thickness) generated from a high purity carbon cord source (Electron Microscopy Sciences) using a Quorum Q150R ES sputter coater (Quorum Technologies ...
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bioRxiv - Microbiology 2022Quote: Purified eCIS samples were briefly centrifuged to remove impurities and 5 μL of supernatant was applied onto freshly glow discharged carbon-coated copper grids (CF400-CU, 6 nm, 400 mesh, Electron Microscopy Sciences). Samples were allowed to adsorb for 2 minutes before being blotted off by filter paper and grids were washed twice with filtered distilled water ...
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bioRxiv - Biochemistry 2023Quote: ... protein was diluted to a concentration of 25 nM and applied to CF400-copper ultra-thin support carbon grids (Electron Microscopy Sciences) that were glow discharged with a PELCO easiGlow (Ted Pella ...
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bioRxiv - Physiology 2023Quote: ... The resulting blocks were trimmed and sectioned into 60 nm thin slices with a Reichert-Jung Ultracut E ultramicrotome using a diamond knife (DiATOME Electron Microscopy Sciences). Silver-appearing sections were placed on a 150 µm mesh copper/rhodium grid (Plano GmbH) ...
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bioRxiv - Cell Biology 2024Quote: ... Ultra-thin serial sections (60 nm) were cut with Ultramicrotome EM UC6 and stained with Leica Ultrostainer with 0.5% Uranyl acetate (Electron Microscopy Sciences#22400) and Ultrostain-2 3% Lead citrate (Leica Microsystems#16707235) ...
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bioRxiv - Zoology 2024Quote: ... 50-nm-thick sections were cut with an Ultra diamond knife and transferred on carbon-coated copper grids with a hole size of 35/10 nm (S35/10, Quantifoil, Electron Microscopy Sciences). Images were recorded with an analytical electron microscope (JEM-2200FS ...
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bioRxiv - Biochemistry 2024Quote: Negative stain grids were made by sputtering a 20 nm amorphous carbon film on the rhodium side of 400-mesh copper-rhodium grids (Electron Microscopy Sciences) pre-coated with a formvar surface in a Leica EM ACE200 Vacuum Coater ...
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bioRxiv - Biochemistry 2024Quote: Cryo-grids were made by evaporating a 20 nm gold layer with ∼4 µm holes on the rhodium side of 400-mesh copper-rhodium grids (Electron Microscopy Sciences) in a Leica EM ACE200 Vacuum Coater ...
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bioRxiv - Biophysics 2024Quote: ... The grids were then incubated (face down) on 150 μL drops of 6 nm colloidal gold conjugated goat-anti-rabbit IgG (Electron Microscopy Sciences), diluted 1:20 in PBS (with 0.1% BSA) ...
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bioRxiv - Cell Biology 2024Quote: Ultrathin sections (70 nm) were cut on an RMC Ultramicrotome (PowerTome) using a diamond knife and recovered to 200 mesh nickel grids (Electron Microscopy Sciences), followed by post-staining with UranylLess (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... Ultrathin sections at 70 nm were generated using a Leica ultramicrotome and collected onto 300 mesh hexagonal copper grids (Electron Microscopy Sciences). Thin sections were imaged using a Zeiss/Leo922 electron microscope equipped with an energy filter operated at 200kV.
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bioRxiv - Developmental Biology 2024Quote: ... Ultra-thin sections (50-70 nm) from the embedded embryos were mounted on copper grids (Formvar 400 mesh; Electron Microscopy Science©) and stained with 2% uranyl acetate for 10 min (Woods and Stirling ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... 6-8 μl of 1 nM DNA origami sample was wicked onto a glow-discharge-cleaned copper grid (Electron Microscopy Sciences, Hatfield, PA) and incubated for 5-10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Neuroscience 2024Quote: ... Melatonin stock solutions of 0.1 mM or 0.01 mM (for a final concentration of 1 nM) were prepared by adding melatonin (Selleckchem, Catalog No. S1204, 10mM) to DMSO (Electron Microscopy Sciences 13390). To each dish containing 50 ml of fish water ...
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bioRxiv - Biophysics 2020Quote: Solutions of extruded vesicles were mixed with 6 nm colloidal gold fiducial markers (Aurion, Wageningen, Netherlands) and applied to glow-discharged C-flat holey carbon grids (Electron Microscopy Science, Hatfield, PA) or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and sections were cut in 80 nm thin sections and 250 nm thick sections with a Leica UC6 ultramicrotome and placed on Rhodium-plated copper slot grids (Electron Microscopy Sciences, Hatfield, PA) for thin section transmission electron microscopy (TEM ...
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bioRxiv - Microbiology 2020Quote: ... The sample was then mounted on a SEM sample holder with double-sided carbon tape and sputter-coated with 5 nm of Pt/Pd (EMS 150 T S Metal Sputter Coater, Electron Microscopy Science, Hatfield, PA). Using the Zeiss SmartSEM software ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 50 nm ultra-thin sections were obtained with a Leica UC7 microtome and collected on Formvar-coated copper grids (Electron Microscopy Science, Hatfield, PA). Specimens were stained with 1 % uranyl acetate and 2.5 % lead citrate and imaged using a JEOL JEM 1010 transmission electron microscope with an AMT XR-16 mid-mount 16 mega-pixel CCD camera ...
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bioRxiv - Genetics 2020Quote: ... Austria) and sputter coated with palladium/gold with a thickness of 50 nm in an EMS 575X sputter coater (Electron Microscopy Sciences, Hatfield, PA). The samples were imaged using a ZEISS Crossbeam 540 FIB-SEM microscope (Carl Zeiss Microscopy GmbH ...