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Citations for Electron Microscopy Sciences :
1401 - 1450 of 1471 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: 100.106 haploid HAP1 cells at 60 % confluency were treated for 72 h with 0.3 mg/mL ethyl methanesulfonate (EMS, Sigma-Aldrich) directly added to the cell medium ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2020Quote: ... The mitotic cells were collected by mitotic shake-off and then seeded onto round 25 mm cover glasses (Electron Microscopy Sciences) and placed onto the cell-holding chamber of an LSM510-Meta confocal microscope equipped with a CO2 incubator and a heating chamber ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with 1X PBS and fixed in 1X PBS containing a mixture of 4% paraformaldehyde (Electron Microscopy Sciences, 15710) and 0.2% Glutaraldehyde (Euromedex ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Microbiology 2024Quote: ... Parasites 8 h after induction of the knock sideways (with 250 nM rapalog) and the control cells grown without rapalog were Percoll-enriched and fixed with 2.5 % glutaraldehyde (Electron Microscopy Sciences) in 50 mM cacodylate buffer pH 7.4 for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with cold PBS for 1 minute and then fixed with 4% PFA (Electron Microscopy Sciences: 157-4) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were dehydrated in graded series of ethanol on ice and embedded in LR White (Electron Microscopy Sciences, Hatfield, PA). The samples were polymerized under UV light (360nm ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots (10 µL) of fixed cells were then loaded onto PTFE printed glass slides (cat#63429-04 from Electron Microscopy Sciences) with 4-mm diameter wells and allowed to air-dry ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on 12mm round coverslips (corning) and were fixed with 4% EM-grade paraformaldehyde (Electron Microscopy Science, Hatfield, PA) in PBS pH7.4 for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed thrice with PBS and smear dried on a Poly-L-lysine coated slide (Electron Microscopy Science: 63410), mounted with Prolong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature with 4% PFA (Electron Microscopy Sciences) diluted in PBS and permeabilized with 70% ethanol overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed once with 1X DPBS(-) and fixed with 4% PFA diluted from a freshly opened 16% PFA (Electron Microscopy Sciences) ampoule ...
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bioRxiv - Developmental Biology 2021Quote: SMG organoid and cell cultures grown on Nuclepore filters (Cytiva #110405) or glass were fixed with 4% paraformaldehyde (Electron Microscopy Sciences #15710) in 1xPBS for 20 minutes or in -20°C methanol for 18 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Migrated cells attached to the lower surface of the inserts were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Incubation solution was then removed and cells were fixed with a 3.2% Paraformaldehyde solution made in PBS (Electron Microscopy Sciences, Hatfield, PA) for 15 min at RT ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... bigemina indirect immunofluorescence assays were performed on >10% parasitemia bovine red blood cells by air drying drops in 10-well slides (Electron Microscopy Sciences), followed by 100% methanol fixation for 5 min at -20°C ...
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bioRxiv - Microbiology 2019Quote: ... Gelatine blocks were prepared by spinning down this cell suspension at room temperature for 10 min at ∼ 5000 × g and an aliquot of the cell pellet was taken and made it to adhere at the bottom of the gelatine capsule (Electron Microscopy Sciences) and then capsule was filled with 100% LR White resin ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Sorted germ cells and SPZEJ were processed as described previously (51, 71) and attached to UltraStick/UltraFrost Adhesion slides (Electron Microscopy Sciences). Samples were fixed in 4% PFA in PBS for 15 min before antigen retrieval in three consecutive 5-min incubations with boiling citrate (10 mM at pH 6) ...
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Immunology 2021Quote: ... An aliquot of mononuclear and granulocyte cells was processed in a cytospin and stained using Diff Quick (26096-25, Electron Microscopy Sciences). Cell differentials were counted ...
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bioRxiv - Neuroscience 2019Quote: ... Retinas were washed and mounted on microscope slides ganglion cell layer-up in Flourogel in TRIS buffer (Electron Microscopy Sciences, 17985-11).
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Resuspended cells were loaded on Concanavalin A-treated coverslip (#1.5 Micro Coverglass −25 mm Diameter, Electron Microscopy Sciences, Cat. No. 72225-01) assembled on imaging cell chamber (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: Cultures were centrifuged and the cells fixed for at least 4 h at room temperature in 10 volumes of 4% paraformaldehyde (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were dehydrated in increasing concentrations of ethanol (50%-100%) at room temperature and embedded in EPON resin (Electron Microscopy Sciences). Ultrathin sections (70-80 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
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bioRxiv - Cell Biology 2023Quote: ... and PFF treatment was conducted at the indicated concentrations and durations prior to fixing cells with 4% Paraformaldehyde (EMS catalog 15710-S) in DPBS (Corning catalog ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the cell suspension was pipetted onto precoated Laminin coverslips at 150-170 μl on each coverslip (Cat#: 72298-01, Electron Microscopy Science), placed in 35 mm petri dishes ...
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bioRxiv - Cell Biology 2023Quote: ... The sapphire disks with affixed cells were flat-embedded onto a Teflon-coated glass microscope slide and covered with a Thermanox coverslip (Electron Microscopy Sciences). Resin was polymerized at 60°C for 48 h and the sapphire disks were excised ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with 1X PBS and then mounted with Fluoro-Gel mounting media (Electron Microscopy Sciences #50-247-04). Immunostained neurons were imaged on a Nikon Eclipse Ti-E inverted epifluorescence microscope equipped with CFI Plan Apochromat VC 100X oil (NA 1.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...