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Citations for Electron Microscopy Sciences :
1301 - 1350 of 1392 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Bioengineering 2024Quote: A 20 µL droplet of 10 nM origami solution diluted in 1×TAE buffer sup- plemented with 12.5 mM MgCl2 was deposited onto a freshly cleaved mica substrate (Electron Microscopy Sciences). The sample was incubated for 1 minute ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Neuroscience 2024Quote: ... slices were collected and fixed for 1 hour at room temperature in 4% PBS-paraformaldehyde (Electron Microscopy Sciences, 50-980-495) and incubated for 20 minutes at 4°C in Clark’s solution (95% ethanol/5% acetic acid) ...
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bioRxiv - Microbiology 2024Quote: 3 µl of sheath preparation sample was incubated for 1 min on glow-discharged carbon-coated formvar grids (Electron Microscopy Sciences). To stain the sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was added to immobilize rosettes (300 µL of a 1:2,000 dilution of a 16% PFA stock solution; Electron Microscopy Sciences 15714 ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Neuroscience 2020Quote: ... Shi/Shi:Rag2-/- mice grafted with control and RRMS hiOLs (n=4 per group) were perfused with 1% PBS followed by a mixture of 4% paraformaldehyde/5% glutaraldehyde (Electron Microscopy Science) in 1% PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mm diameter PDMS wells were passively adhered to #1 9 mm x 9 mm glass coverslips (Electron Microscopy Sciences #72190-09). Wells were passivated overnight using vapor deposition of Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931 ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2021Quote: 8 μl of 1 x 1010 pfu/mL of phages was applied to a copper mesh grid coated with formvar and carbon (Electron Microscopy Sciences) for 2 minutes and then gently blotted off with a piece of Whatman filter paper ...
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bioRxiv - Neuroscience 2022Quote: ... Grids were washed with two drops of double-distilled H2O and stained with two drops of freshly prepared 1% (w/v) uranyl formate (Electron Microscopy Sciences). The samples were analysed at room temperature using a Tecnai Spirit (FEI ...
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bioRxiv - Microbiology 2022Quote: ... Washed tardigrades were dried onto one field of a 76 × 26 × 1 mm 10-well (6mm) polytetrafluoroethylene-printed slide (Electron Microscopy Sciences). Fixation of specimens was performed by adding 30 μL of ice-cold 3:1 4% paraformaldehyde:1x phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... Sample aliquots (40 µl) were taken at regular time points over 40 h and fixed with 10 µl of 1% glutaraldehyde each (Electron Microscopy Sciences). The number of food vacuoles in 10 cells was counted under light microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were dissociated and 75,000 cells were replated overnight in 2D on 12 mm round #1 coverglass (Electron Microscopy Sciences 72231-01) coated with 75 μg/ml Poly-D-lysine followed by 3% Matrigel ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Physiology 2022Quote: ... freshly isolated pituitary glands were fixed with 2.5% formaldehyde/glutaraldehyde in 0.1 M Sodium Cacodylate Buffer (pH 7.4, Emsdiasum) and 1% OsO4 (Electron Microscopy Science, Cat No. 15949), followed by dehydration and staining with uranyl acetate/lead citrate ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... In-house graphene grids were prepared from Trivial Transfer Graphene sheets (ACS Material) and QUANTIFOIL R2/1 mesh 300 gold (Electron Microscopy Sciences) by following a protocol introduced by Ahn et. ...
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bioRxiv - Biochemistry 2022Quote: Washed and deglycosylated RBCs were diluted 1:100 and added to carbon coated 400 mesh copper grids (CF400-CU, Electron Microscopy Sciences) that had been glow discharged and pre- coated in poly-d-lysine solution ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... made in double distilled H2O (ddH2O) and crosslinked by 1% glutaraldehyde (diluted from 50% glutaraldehyde, Electron Microscopy Sciences, catalog #16320, Hatfield, PA). 40 µm sections were cut on a vibratome (Leica VT1000S ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were washed five times for 10 min each with sterile deionized water and then transferred to fresh solution containing 1% (w/v) thiocarbohydrazide (EMS, Cat# 21900) in deionized water and incubated for 1 hour under rotation at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... were collected on nickel grids using a Leica EM UC7 ultra microtome and stained with uranyl acetate (1%, w/v) (uranyl acetate dihydrate, Electron Microscopy Sciences) and Reynolds lead citrate71 (Lead nitrate (Thermofisher ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were then counterstained with Hoechst 33342 (1:10,000) and mounted using Fluoro-gel in TES Buffer (Electron Microscopy Sciences, 17985-30). The slides were imaged using a Zeiss Observer D1 microscope with a 40x objective.
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bioRxiv - Biophysics 2024Quote: ... 200 mM NaCl and 0 – 0.01% LMNG or 1 mM CHAPSO) was applied to a 45 s glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) or R1.2/1.3 300 copper mesh quantifoil grid (Quantifoil Micro Tools ...
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE-1 and worm samples were washed three times with pure anhydrous acetone and infiltrated with Epon/Araldite (EMS, Hatfield, USA) at increasing concentrations of resin (resin:acetone ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...