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Citations for Electron Microscopy Sciences :
1051 - 1100 of 1175 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... mice were perfused transcardially with heparinized saline (10 units mL−1 of heparin) and 4% paraformaldehyde (PFA) that was freshly prepared from 32% PFA aqueous solution (Cat# 15 714, EMS), using a peristaltic pump at a rate of 7 mL min−1 ...
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bioRxiv - Cell Biology 2024Quote: ... Sapphire discs with attached frozen hTERT-RPE-1 cells and type-A planchettes containing worms were transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µL of resuspended cells were pipetted onto agarose pads in 13 x 1 mm silicone isolator wells (Electron Microscopy Sciences) and covered with a No ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resin infiltrated in ethyl alcohol and Spurr’s resin epoxy mixtures using an automated EMS Lynx 1 EM tissue processor (Electron Microscopy Sciences). Processed samples were infiltrated with two changes of fresh Spurr’s resin for 24 hours and the samples were then oriented in the resin ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Systems Biology 2020Quote: ... rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Systems Biology 2020Quote: ... the La2-DAB solution was removed and cells were rinsed with 0.1M sodium cacodylate buffer pH 7.4 (5X1min) on ice and then were posted fixed with 1% osmium tetroxide (19150, Electron Microscopy Sciences) containing 0.8% potassium ferrocyanide ...
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bioRxiv - Neuroscience 2021Quote: ... or to fit a stack of custom laser-cut quartz glass coverslips (three coverslips with #1 thickness each (Electron Microscopy Sciences), cut to a ‘D’-shape with maximum dimensions of 5.5 mm medial-lateral and 7.7 mm anterior-posterior ...
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bioRxiv - Cell Biology 2021Quote: ... cells were gently washed with 30 μL of PBS and fixed with 1% (v/v) paraformaldehyde (Electron Microscopy Sciences 15710-S) diluted in PBS at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified TTP at 1.5 mg mL-1 was applied to a 400-mesh copper lacey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Frozen samples were then opened under liquid nitrogen and transferred to cryo-vials filled with anhydrous acetone containing 1% (w/v) osmium tetroxide (EMS, USA) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with cold PBS for 1 minute and then fixed with 4% PFA (Electron Microscopy Sciences: 157-4) for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Developmental Biology 2023Quote: ... infiltrated with Embed-812 resin series by 20% increments in acetone (each step was 1 h) and embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60°C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-rabbit IgG (H+L) Ultrasmall EM grade 0.8nm colloidal gold (1:100 IG; Aurion, Electron Microscopy Sciences Cat# 25101).
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bioRxiv - Biochemistry 2023Quote: ... 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min ...
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bioRxiv - Bioengineering 2023Quote: ... A 3-μL droplet of the L/D-GQD solution (0.1 mg·mL-1) was placed on the carbon-coated copper TEM grid (CF200-Cu-25; Purchased from Electron Microscopy Sciences, PA) and allowed to dry in the air ...
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bioRxiv - Developmental Biology 2023Quote: Wing imaginal discs dissected from late third instar larvae in 1×PBS (pH 7.4) were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 40[minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were placed (section face-down ...
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bioRxiv - Bioengineering 2024Quote: A 20 µL droplet of 10 nM origami solution diluted in 1×TAE buffer sup- plemented with 12.5 mM MgCl2 was deposited onto a freshly cleaved mica substrate (Electron Microscopy Sciences). The sample was incubated for 1 minute ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... 1 mL of culture was fixed at ambient temperature in the dark (1-2 h) with paraformaldehyde 3.8% (PFA, Electron Microscopy Sciences). Cells were then centrifuged 3 min at 8000 g and washed 3 times with 500 µL PBS ...
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bioRxiv - Microbiology 2024Quote: ... RAW264.7 and THP-1 cells were thawed and seeded as mentioned earlier on 6 well plates containing #1.5H glass cover slips (Electron Microscopy Sciences). Cells were grown and maintained as described above ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was added to immobilize rosettes (300 µL of a 1:2,000 dilution of a 16% PFA stock solution; Electron Microscopy Sciences 15714 ...
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bioRxiv - Neuroscience 2024Quote: ... slices were collected and fixed for 1 hour at room temperature in 4% PBS-paraformaldehyde (Electron Microscopy Sciences, 50-980-495) and incubated for 20 minutes at 4°C in Clark’s solution (95% ethanol/5% acetic acid) ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS twice followed by fixation and permeablization in 1 ml PBS containing 2% PFA (Electron Microscopy Sciences) and 0.1% saponin (Sigma–Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were mixed with 0.5 µl of 0.04 mM Lauryl maltose neopentyl glycol (LMNG) solution immediately before sample deposition onto QUANTIFOIL® 2/1 400 mesh grids (EMS), which were plasma cleaned for 10 s using Gatan Solarus 950 Plasma system (Ar/O2 gas mixture) ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Microbiology 2022Quote: ... grids were washed by contact with 2 drops of milliQ water and were negatively stained with 20 µl of 1% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were diluted to ~20 μg mL−1 and applied for 60 s to glow discharged Cu grids with continuous carbon film (300 mesh) (Electron Microscopy Sciences). Excess sample was blotted using a Whatman filter paper and stained for an additional 60 s using Nano-W (Nanoprobes) ...
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bioRxiv - Biophysics 2021Quote: ... 2uL of dialyzed sample was then applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 200- or 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were subsequently blotted for 2 or 3 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were washed with two 20 μL drops of ultrapure water and then stained with two 20 μL drops of 1% (w/v) uranyl acetate (Electron Microscopy Sciences); the first for 10 sec and the second for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... the flow chamber was washed with a second reaction mix containing 25 µM tubulin before fixing with 1% glutaraldehyde (Electron Microscopy Sciences) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a slot (1 × 2 mm) EM copper grid covered with a formvar film (FF2010-Cu, Electron Microscopy Sciences, USA). Sections were covered by 0.1 μm TetraSpeck microsphere fiducials (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mm diameter PDMS wells were passively adhered to #1 9 mm x 9 mm glass coverslips (Electron Microscopy Sciences #72190-09). Wells were passivated overnight using vapor deposition of Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931 ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue was lysed until no visible pieces remained just prior to use in 15 μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) with a pestle (Electron Microscopy Sciences, 64788) and a LabGen 7B homogenizer (ColePalmer ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated with the DNA intercalator Iridium (Fluidigm, 201192A) at a dilution of 1:4,000 in 2% paraformaldehyde (Electron Microscopy Sciences, 15714) in DPBS (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... Samples of the complex (2 μL) were applied to glow-discharged (1 min, easiGlow, Pelco) R2/2 300-mesh Quantifoil holey carbon grids (Electron Microscopy Sciences). The grids were blotted for 2 s with Whatman 41 filter paper and flash-frozen by plunging into liquid ethane using a Leica EM CPC manual plunger ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
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bioRxiv - Biochemistry 2021Quote: ... 1.8 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu, Electron Microscopy Sciences) and plunge frozen into liquid ethane using a FEI Vitrobot Mark IV using a blot time of 25-30 seconds and a blot force of 22 ...