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Citations for Electron Microscopy Sciences :
951 - 1000 of 1010 citations for 6H Purin 6 imine 1 ethoxy 7 ethyl 1 7 dihydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was washed with PBS and fixed in 1 mL ice cold 4% PFA (Electron Microscopy Science, Hatfield, PA, USA) diluted in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed in cytoskeletal buffer and fixed for 1 hour at room temperature in 4% formaldehyde (Cat# 1570, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were washed five times for 10 min each with sterile deionized water and then transferred to fresh solution containing 1% (w/v) thiocarbohydrazide (EMS, Cat# 21900) in deionized water and incubated for 1 hour under rotation at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... were collected on nickel grids using a Leica EM UC7 ultra microtome and stained with uranyl acetate (1%, w/v) (uranyl acetate dihydrate, Electron Microscopy Sciences) and Reynolds lead citrate71 (Lead nitrate (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... made in double distilled H2O (ddH2O) and crosslinked by 1% glutaraldehyde (diluted from 50% glutaraldehyde, Electron Microscopy Sciences, catalog #16320, Hatfield, PA). 40 µm sections were cut on a vibratome (Leica VT1000S ...
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bioRxiv - Plant Biology 2019Quote: ... using fine forceps and a 27-gauge needle as a scalpel, developing seeds were dissected from siliques and immediately immerged in fix solution (1×PBS, 4% formaldehyde (Electron Microscopy Sciences, 15713), and 0.4% dimethyl sulfoxide ...
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bioRxiv - Microbiology 2019Quote: Equal numbers of bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29 ...
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bioRxiv - Neuroscience 2021Quote: Coronal sections (n=2 mice, P30) and whole mounts (n=2 mice, P28) were incubated with Immunosaver (1:200; EMS, Fort Washington, PA) for 20 min at 60°C ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were put in propylene oxide for 1 h and infiltrated overnight in a 11 mixture of propylene oxide and Spurr’s low viscosity resin (Electron Microscopy sciences, Hatfield, PA). The cells were embedded in Spurr’s resin and polymerized at 60°C for 48 h ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in 1 ml fixative (40 mM KHPO4 pH 7.0 containing 1% EM grade glutaraldehyde and 0.2% EM grade formaldehyde from Electron Microscopy Sciences (EMS, Munich, Germany) and incubated for 3 min.
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspended in 100 mM sucrose and the cell suspension distributed onto slides (Matsunami, S9901) covered with 1% PFA in H2O (Electron Microscopy Science, 15713) with 0.2% Triton X-100 (pH 9.2-9.4) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal media was carefully aspirated from all wells and coverslips were briefly rinsed with ice cold 1× PBS with Ca2+/Mg2+ followed by fixation in freshly made 4% PFA (Electron Microscopy Sciences: 15713) in 1x PBS for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... 3 mL of cells was harvested by centrifugation for 3 min at 1500 × g at room temperature and fixed for 30 min in 1 mL of 2.5% (v/v) glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) in 0.1 M cacodylate buffer (Electron Microscopy Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of SKD3 with 1 mM ATP was applied to glow-discharged 400 mesh formvar carbon film grids with copper supports (Electron Microscopy Sciences, EMS) and stained with a 5 mg/ml uranyl formate solution ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were resuspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (46) ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
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bioRxiv - Biochemistry 2023Quote: 5 μl of PFF solution (∼1 mg/mL) was spotted on a freshly glow-discharged 300-mesh carbon coated formvar grid (Electron Microscopy Sciences, USA) and incubated for 3 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were suspended in sodium phosphate buffer (pH 6.8) and treated for 15 min at room temperature with 1% monomeric formaldehyde (Electron Microscopy Sciences Cat #15710) as previously described (29) ...
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bioRxiv - Microbiology 2023Quote: ... Ileum pieces were rinsed 3 times for 5 minutes in PB buffer and then postfixed with a fresh mixture of osmium tetroxide 1% (EMS, Hatfield, PA, US) with 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then mounted on 12 mm #1 thickness poly-d-lysine coated round coverslips (Neuvitro Corporation, GG-12-pdl) and fixed in fresh 4% paraformaldehyde (Electron Microscopy Sciences, 15710) in 1× PBS with 0.3% Triton detergent (0.3% PBST) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... anesthetized larvae were moved to a rectangular plate (4.5 cm x 3 cm x 1 cm) coated with Sylgard (Electron Microscopy Sciences, Hatfield, PA). After removing excess water ...
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bioRxiv - Biophysics 2023Quote: ... samples were taken directly from the microplates (section 2.3) and deposited for 1 min on a previously negative glow-discharged carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, Pennsylvania). The grids were then washed with water and stained with phosphotungstic acid ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with ice-cold 1% phosphatase inhibitor cocktail/PBS once and then fixed with freshly prepared 4% PFA (Electron Microscopy Science, 15710) for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 3 µL complex was mixed with 1 µL 20 µM lauryl maltose neopentyl glycol (LMNG) and immediately applied to glow discharged C-Flat™-2/1-3Cu-T50 (Electron Microscopy Sciences) holey grids at 4 °C and 100 % humidity inside a Vitrobot Mark IV ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed for 1 hour at room temperature (RT) in Paraformaldehyde (PFA) 4% in PBS 1X (32% stock, Electron Microscopy Sciences, 15714). They were washed 4 times for 10min in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Bioengineering 2022Quote: ... 4μL of ~1 nM purified DNA nanostructure was deposited onto a copper TEM grid coated with carbon and formvar (Electron Microscopy Sciences, Hartfield, PA) and incubated for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: Overnight cultures were diluted 1:10 in fresh LB medium and were added to 48-well microtiter plates containing autoclaved Aclar fluoropolymer film (Electron Microscopy Sciences, Hatfield, PA). Biofilms were grown for 48 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were negatively stained with 1% uranyl acetate in Tris NaCl refolding buffer and fixed onto 400 mesh copper grids (Electron Microscopy Sciences, Hatfield, PA). TEM imaging was done at 100x magnification with a JEOL 1400 electron microscope ...
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bioRxiv - Cell Biology 2021Quote: ... a film of germanium (Structure Probe, Inc., West Chester, PA) was applied to glass coverslips of 25 mm diameter and thickness #1 (Electron Microscopy Sciences, Hatfield, PA) as previously described (Heckman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... were adsorbed for 30 s (for high salt conditions) to 1 h in a humid chamber (for low salt conditions) on a glow-discharged carbon-coated grid (Electron Microscopy Sciences, CF300-CU). For low salt conditions ...
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bioRxiv - Biochemistry 2022Quote: ... for four hours at room temperature (ref: “C. elegans: a practical approach”, chapter “Conventional Genetics” by Jonathan Hodgkin, “Protocol 1-EMS Mutagenesis”, pages 252-253) and selected for increased survival NGM-OP50-1 plates with on 5 mM −7.5 mM DTT ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Cell Biology 2021Quote: ... were applied to carbon-coated and glow discharged (2 minutes at 7x10-1 mbar) 300-mesh copper EM grids (Electron Microscopy Sciences; CF300-CU) and incubated for four minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Surface colonization was performed by washing and diluting an LB overnight culture 1:100 into fresh SCFM and dispensing 100 µl into coverslip attached open incubation chambers (Electron Microscopy Sciences, 70333-42). The coverslips were incubated in parafilm sealed sterile petri dishes at 37°C and the media was gently exchanged every 4 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was added to the plates mentioned above and incubated for 1 h at 37°C with 5% CO2 after which cells were fixed using 4% PFA (Electron Microscopy Sciences, Hatfield, PA) and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Five µL of OMVs that had been diluted 1:10 in saline were added to a glow discharged 200 mesh formvar/carbon grid (Electron Microscopy Sciences, FCF200-Cu) and incubated for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... thick resin sections (250 nm) were cut and placed on a slot (1 × 2 mm) EM copper grids covered with a formvar film (Electron Microscopy Sciences, FF2010-Cu). Grids were decorated with fiducial marker and stained with Hoechst to visualize nuclear regions ...
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bioRxiv - Biochemistry 2024Quote: Purified receptor was diluted to ∼5 ug mL−1 and applied to freshly glow-discharged EM carbon coated copper grid (Electron Microscopy Sciences, CF400-Cu,) for 30 sec ...
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bioRxiv - Developmental Biology 2023Quote: ... After rinsing in 0.1 M PBS at room temperature (10 min x 3) and post-fixed in a sequence of aqueous 1% OSO4 (#19150, Electron Microscopy Science, Hatfield, PA) in 0.1 M PBS ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...