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Citations for Electron Microscopy Sciences :
951 - 1000 of 1241 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Grids with the collected monolayers facing up were stained at once with 2% uranyl formate (Electron Microscopy Sciences) in water by depositing 4 μL on the grid and simultaneously blotting the excess solution ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fixation with 2% PFA (w/v; prepared from a 20% stock solution, Electron Microscopy Sciences: 15713) in cell culture medium for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... following which they were placed in a mixture (500 μL) of 2% OsO4 (Electron Microscopy Sciences, cat#19150) and 0.1 M sodium cacodylate buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were fixed in 2% PFA/2.5% glutaraldehyde/0.1 M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Lateral ventricle walls were fixed for 18 h at 4 °C othen subdissected into anterior ...
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bioRxiv - Neuroscience 2020Quote: ... unbuffered OsO4 aqueous solution for 2 hours and lead aspartate solution (0.03 M, pH 5.0 adjusted by KOH, EMS) at 50 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were intracardially perfused with NaCl 0.9% followed by PFA 2% (Electron Microscopy Sciences 15713, diluted in PBS). Brains were post-fixed in PFA 2% and cryoprotected in 20% sucrose overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were stained by a 2 minute bath in 0.1% cresyl violet acetate (Electron Microscopy Sciences #26089-01), followed by a 2 minute rinse in distilled water ...
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bioRxiv - Microbiology 2020Quote: ... and stained with 2% uranyl formate (pH 5.5– 6.0) on thin carbon-layered 400 mesh copper grids (EMS)46 ...
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bioRxiv - Biophysics 2024Quote: 3 μL of purified MscS between 0.1 and 0.7 mg/mL was applied to a glow discharged Quantifoil R 1.2/1.3 + 2 nm C 400 Cu mesh grid (EMS), a holey grid with a thin 2 nm continuous carbon film ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos prepared for immunostaining were fixed overnight at 4°C in 2% PFA (Electron Microscopy Sciences, Catalog #15710). Embryos were either embedded in 4% low-melt agarose/PBS + 0.1% Tween and cut into 200um sections using a Leica VT1200 or stained as intact whole-mounts ...
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bioRxiv - Physiology 2023Quote: ... Ovaries used for MIMS were cut in half and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in LR white (EMS) ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with pentobarbital and perfused with 2% paraformaldehyde (PFA, Paraformaldehyde Granular, Electron Microscopy Sciences, Hatfield, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... (2) fixed with 1.5% paraformaldehyde and 1.5% glutaraldehyde in 0.1M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA, USA), pH 7.3 for 1 h at room temperature followed by 3 washes with 0.1M sodium cacodylate ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed in freshly diluted 2% paraformaldehyde (PFA) in PBS (16% PFA purchased from EMS Cat# 15710) for 20 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 10 μL of parasite-containing media from each flask was added to 90 μL of 2% PFA (EMS) to fix and dilute the parasites at a 1:10 ratio in a 96-well flat bottom plate ...
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bioRxiv - Immunology 2024Quote: ... coated grids (R2/2, Au 200-mesh London Finder grid coated with extra thick carbon, Electron Microscopy Sciences) for another 24 hours to allow sufficient adherence ...
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bioRxiv - Immunology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Biochemistry 2024Quote: Two μl of each FAN1-PCNA-DNA complex sample was applied to a 300-mesh copper Quantifoil R 1.2/1.3 + 2 nm holey carbon grid (EMS), which was previously glow-discharged for 90 seconds at 15 mA current using an easiGlow (PELCO) ...
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bioRxiv - Cell Biology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... DE) was diluted to 5 μg/mL in assay buffer: 1X PBS with 0.1% acetylated BSA (Electron Microscopy Sciences, Hatfield, PA) in a 96-well plate and loaded onto streptavidin biosensors (FortéBio ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at the Pole Facultaire de Microscopie Electronique at the University of Geneva as described 5 except uranyl acetate staining was replaced with Reynold’s (lead citrate, Electron Microscopy Sciences), and 20 nm sections were used ...
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bioRxiv - Biophysics 2021Quote: ... We fixed the cells by incubating 5 mL of culture with electron microscopy grade glutaraldehyde (16300, Electron Microscopy Sciences, Inc., PA) at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Immunology 2020Quote: ... ~ 5 μL of the sonicated samples were drop cast onto 300-mesh formvar carbon-coated copper grids (Electron Microscopy Sciences, UK) and negatively stained with UranyLess (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS with 0.2% Triton X-100 and mounted onto slides with DAPI Fluoromount (#17984-24, Electron Microscopy Sciences) before visualization with an EVOS imaging system (Thermo Fisher Sci).
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were mounted on studs using silver paint and coated with 5 nm of Palladium (sputter coater EMS150TS; Electron Microscopy Sciences). Samples were imaged at 5 kV on a FEI Magellan 400 XHR Field Emission Scanning Electron Microscope at the Stanford Nano Shared Facilities.
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... was infused into the well of the chamber and covered with a 5-mm diameter glass coverslip (7229605, Electron Microscopy Sciences). The skin was sealed with VetbondTM Adhesive ...
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bioRxiv - Biophysics 2023Quote: ... 5 µls were applied to a glow discharged 1.2/1.3 CF-1.2/1.3-3Au-50 (Electron Microscopy Sciences, part # AUFT313-50) cryo-EM grid and allowed to absorb for 30 sec ...
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bioRxiv - Neuroscience 2023Quote: ... 70-nm thick sections from these areas were generated (4-6 levels,[∼[5–10 µm apart) and collected on silicon wafers (EMS, Pennsylvania) for imaging on a Zeiss Crossbeam 350 focused ion beam-scanning electron microscope (FIB-SEM) ...
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bioRxiv - Microbiology 2023Quote: ... Slides were subsequently washed three more times for 5 minutes by immersion in 0.1% Tween-20 in PBS and mounted in EMS Shield Mount with DABCO (Electron Microscopy Sciences). Imaging was performed using a Carl Zeiss LSM710 confocal microscope at the microscopy imaging center (MIC ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of EVs was placed onto glow discharged 200-mesh formvar/carbon copper grids (Electron Microscopy Sciences, Hatfield Township, PA). The EVs were washed two times with water and then fixed in 2.5% glutaraldehyde ...
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bioRxiv - Plant Biology 2024Quote: Aliquots (5 µL) of resuspended P40 pellets were spotted onto Formvar and carbon-coated copper electron microscopy grids (Electron Microscopy Sciences) that were glow-discharged at 15mA for 60 seconds prior to application of samples ...
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bioRxiv - Neuroscience 2024Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... main inflorescences (not side branches) were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...