Labshake search
Citations for Electron Microscopy Sciences :
951 - 989 of 989 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... Approximately 20,000 seeds homozygous for the SCRpro::GFP-SCR transgene were treated overnight at room temperature with 10 mL 0.1% ethyl methanesulfonate (EMS, Sigma M0880, MO, USA). After thorough washing ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then stained with wheat germ agglutinin for 10 min prior to fixing with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min and permeabilization with 0.5% Triton-X 100 for 10 min ...
-
bioRxiv - Microbiology 2019Quote: ... Negative staining of the fractions from OptiPrep density gradients was performed by adsorbing 10 μL of each fraction to glow-discharged 400 mesh ultra-thin carbon coated grids (EMS CF400-CU-UL) for two minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.4 μm Pore Polyester Membrane Insert fixed to a custom 3D printed insert (Supplemental Material) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10).
-
bioRxiv - Microbiology 2024Quote: ... the wells were fixed by adding on top of the supernatant 10% formaldehyde (Formaldehyde Aqueous Solution EM grade, 32%, Liquid Electron Microscopy Sciences, # 15700) at a volume-to-volume ratio for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
-
bioRxiv - Neuroscience 2024Quote: Coverslips that were 22m x 22m and size #1 were used as the transparent walls of the chamber (Electron Microscopy Sciences: #72200-10). Rain-X was applied to the inner surface of the coverslips to limit flies from walking on the glass ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... 13985-584-10) and xylazine (20mg/kg, VetOne. 13985-612-50) cocktail and were transcardially perfused with 4% paraformaldehyde (PFA, Electron Microscopy Sciences,15713) in phosphate buffer solution (PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Each explant was mounted onto an O-ring (McMaster Carr 1283N264) fixed into a 60mm dish (Cellstar 628160) with Sylgard 184 silicone elastomer (EMS Catalog #24236-10). Each region was imaged with a 25X objective and cells were counted on a maximal intensity projection ...
-
bioRxiv - Cell Biology 2021Quote: Cells were cultured on cell-tight C-2 cell disks (Sumitomo Bakelite, Tokyo, Japan; #MS-0113K) and fixed for 2 h on ice in 2.5% glutaraldehyde (Electron Microscopy Science, Hatfield, PA; #111-30-8) in 0.1 M phosphate buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
-
bioRxiv - Immunology 2022Quote: Samples (10 µl) were adsorbed to glow-discharged 400 mesh carbon coated ultra-thin grids (Electron Microscopy Sciences 215-412-8400 CF400-CU µL) for 5 min ...
-
bioRxiv - Microbiology 2019Quote: Overnight cultures were diluted 1:10 in fresh LB medium and were added to 48-well microtiter plates containing autoclaved Aclar fluoropolymer film (Electron Microscopy Sciences, Hatfield, PA). Biofilms were grown for 48 h at 37°C ...
-
bioRxiv - Genomics 2021Quote: ... Slides were washed three times in 1x TBS/0.1% Tween-20 for 10 minutes before being incubated with 200 µL 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-487) in a dark humidity chamber at room temperature for 30 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... 10 ml of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Genomics 2022Quote: ... OCT tissue blocks were sectioned into 10 µm thickness sections and placed in the center of poly-L-lysine slides (Electron Microscopy Sciences, 63478-AS), and shipped with dry ice ...
-
bioRxiv - Physiology 2021Quote: ... at 370C and washed in Dulbesco Phospate Buffered Saline - DPBSx1 (Biological Industries, Cat# 02-020-1A).Neurons were than briefly fixed (10 minutes) with 4% paraformaldehyde (PFA, Electron microscopy sciences, Cat #BN15710) in DPBSx1 ...
-
bioRxiv - Neuroscience 2020Quote: ... and placed overnight in a 1:50 dilution of donkey anti–rabbit IgG with bound 10-nm colloidal gold (Electron Microscopy Sciences, catalog 25704) diluted in blocking buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 μl of retromer at concentrations between 5 and 10 μg/mL were applied to continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences, Hatfield, PA) and washed twice with water ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were fixed by immersion in methanol at -200C for 10 minutes followed by fixation in 4% EM grade paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 10 minutes followed by permeabilization and blocking in PBTA Blocking Solution (PBS 1X ...
-
bioRxiv - Microbiology 2022Quote: ... Five µL of OMVs that had been diluted 1:10 in saline were added to a glow discharged 200 mesh formvar/carbon grid (Electron Microscopy Sciences, FCF200-Cu) and incubated for 5 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... After rinsing in 0.1 M PBS at room temperature (10 min x 3) and post-fixed in a sequence of aqueous 1% OSO4 (#19150, Electron Microscopy Science, Hatfield, PA) in 0.1 M PBS ...
-
bioRxiv - Microbiology 2024Quote: ... A 50 µL bacterial suspension was then dropped and immobilized on smooth surface of an AFM-grade mica sheet (Electron Microscopy Sciences, # 71855-10) and air dried ...
-
bioRxiv - Neuroscience 2024Quote: ... the grids were incubated with secondary antibodies conjugated to 10 nm or 6 nm gold particles (Cytodiagnostics, 1:20, and Electron Microscopy Sciences, 1:40) in PBS containing 0.1% gelatin at 21 °C for 1 h ...
-
bioRxiv - Pathology 2019Quote: ... The samples were then sectioned into 5 μm-thick slices and stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols.
-
bioRxiv - Pathology 2021Quote: ... Sections were stained with hematoxylin and eosin (H&E; Fisherbrand™ 517-28-2, Waltham, MA) or safranin-O and fast green (Saf-O; Electron Microscopy Sciences® 20800, Hatfield, PA), following manufacturer protocols ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
-
bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... A 10 μl droplet of the EV-PFA mixture was then allowed to adsorb to a carbon-coated copper grid (Electron Microscopy Sciences, CF200-Cu-25) for 20 min ...
-
bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... the RV particles were adsorbed for 10 min on glow-discharged carbon-coated Parlodion films mounted on 300 mesh per inch copper grids (Electron Microscopy Science, Hatfield, PA, USA). Samples were washed once with distilled water and stained with saturated uranyl acetate (Fluka ...
-
bioRxiv - Neuroscience 2024Quote: ... Poly-l-lysine coated chambers and coverslips were prepared by washing glass microscope slides and glass coverslips with acid alcohol (1% HCl in 70% ethanol) and placing 1:10 0.1% poly-l-lysine (Electron Microscopy Sciences, catalog #19320, Hatfield, PA) in ddH20 on slide for 1 hour before aspirating off and leaving covered in fume hood overnight (Suvarna SK 2013 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The sample attached to the grid was stained by immediately tapping the grid on 2% w/w uranyl acetate (Electron Microscopy Sciences, Hatfield, PA, USA, Cat. No. 22400) solution droplets twice ...
-
bioRxiv - Cell Biology 2019Quote: ... Aliquots of 3.5 μl of the overnight actin/dynamin reaction mixture with 10 nm gold fiducials was loaded onto glow-discharged gold C-flat grids (Electron Microscopy Sciences, CF-1.2/1.-4Au-50) for 20 sec ...